Use of green fluorescent protein as molecular marker for taggingBacillus brevis in soil under the control of a novel constitutive promoter F1

2005 ◽  
Vol 50 (5) ◽  
pp. 437-442 ◽  
Author(s):  
Yunpeng Chen ◽  
Daleng Shen ◽  
Mingjie Yang
Keyword(s):  
Green Fluorescent Protein ◽  
Molecular Marker ◽  
Fluorescent Protein ◽  
Constitutive Promoter ◽  
Green Fluorescent

scholarly journals Exploring Infection of Wheat and Carbohydrate Metabolism in Mycosphaerella graminicola Transformants with Differentially Regulated Green Fluorescent Protein Expression

2001 ◽  
Vol 14 (2) ◽  
pp. 156-163 ◽  
Author(s):  
Eric A. Rohel ◽  
Andrew C. Payne ◽  
Bart A. Fraaije ◽  
Derek W. Hollomon
Keyword(s):  
Green Fluorescent Protein ◽  
Carbon Source ◽  
Promoter Region ◽  
Fluorescent Protein ◽  
Isocitrate Lyase ◽  
Mycosphaerella Graminicola ◽  
Cell Junctions ◽  
Constitutive Promoter ◽  
Wheat Leaves ◽  
Green Fluorescent

A Mycosphaerella graminicola strain transformed with the green fluorescent protein (GFP) downstream of either a carbon source-repressed promoter or a constitutive promoter was used to investigate in situ carbohydrate uptake during penetration of the fungus in wheat leaves. The promoter region of the acu-3 gene from Neurospora crassa encoding isocitrate lyase was used as a carbon source-repressed promoter. The promoter region of the Aspergillus nidulans gpdA gene encoding glyceraldehyde-3-phosphate dehydrogenase was used as a constitutive promoter. Fluorometric measurement of GFP gene expression in liquid cultures of acu-3-regulated transformants indicated that the N. crassa acu-3 promoter functions in M. graminicola as it does in N. crassa, i.e., acetate induced and carbon source repressed. Glucose, fructose, and saccharose triggered the repression, whereas mannitol, xylose, and cell wall polysaccharides did not. Monitoring the GFP level during fungal infection of wheat leaves revealed that acu-3 promoter repression occurred after penetration until sporulation, when newly differentiated pycnidiospores fluoresced. The use of GFP transformants also allowed clear visualization of M. graminicola pathogenesis. No appressoria were formed, but penetration at cell junctions was observed. These results give new insight into the biotrophic status of M. graminicola.

Applications of the green fluorescent protein as a molecular marker in environmental microorganisms

1999 ◽  
Vol 35 (3) ◽  
pp. 187-199 ◽  
Author(s):  
D. Errampalli ◽  
K. Leung ◽  
M.B. Cassidy ◽  
M. Kostrzynska ◽  
M. Blears ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Molecular Marker ◽  
Fluorescent Protein ◽  
Green Fluorescent
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