Statistical analysis of the envelope gene and the prM region of Japanese encephalitis virus: Evidence suggestive of positive selection

1997 ◽  
Vol 76 (2) ◽  
pp. 117-131 ◽  
Author(s):  
Shirish Paranjpe ◽  
Atul Walimbe ◽  
K. Banerjee
Keyword(s):  
Statistical Analysis ◽  
Positive Selection ◽  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Encephalitis Virus ◽  
Envelope Gene

scholarly journals ZBTB38 in Porcine Alveolar Macrophages Positively Regulates the Proliferation of Japanese Encephalitis Virus

2021 ◽  
Author(s):  
Yi Zheng ◽  
Yu-Yong Zhou ◽  
Chun-Xia Chai ◽  
San-Jie Cao ◽  
Qi-Gui Yan ◽  
...  
Keyword(s):  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Alveolar Macrophages ◽  
Encephalitis Virus ◽  
Porcine Alveolar Macrophage ◽  
Envelope Gene ◽  
Interacting Protein ◽  
Btb Domain ◽  
New Information ◽  
Ns3 Protein

Abstract Background Japanese encephalitis (JE) is an important zoonotic disease caused by Japanese encephalitis virus (JEV), and pigs are intermediate host of this disease. Previous studies have confirmed that JEV can proliferate in the respiratory tract of mice and spread through it. Therefore, this study aimed to screen the proteins interacting with JEV on porcine alveolar macrophage cell and verify its role in the proliferation of JEV.Methods and results Porcine alveolar macrophages cell line 3D4/21 were infected with JEV, and obvious cytopathic effect (CPE) was observed. Zinc finger and BTB domain containing 38 (ZBTB38) was screened out as an interacting protein using co-immunoprecipitation assay and validated through knockout and overexpression of ZBTB38 in 3D4/21 cells. The results demonstrated that loss of ZBTB38 function basically had no effect on the attachment and entry processes of JEV, while the transcription level of JEV envelope gene, the expression level of NS3 protein and the number of virions were all significantly down-regulated in the subsequent infection stage. Conclusion Overall, one core conclusion was drawn in this paper that ZBTB38 promotes the proliferation of JEV especially in the middle and late stages of infection. This study provides new information for understanding the pathogenic mechanism of JEV, especially the respiratory transmission caused by JEV infection.

A novel mutation (S227T) in domain II of the envelope gene of Japanese encephalitis virus circulating in North India

2010 ◽  
Vol 139 (6) ◽  
pp. 849-856 ◽  
Author(s):  
S. K. PUJHARI ◽  
S. PRABHAKAR ◽  
R. K. RATHO ◽  
M. MODI ◽  
M. SHARMA ◽  
...  
Keyword(s):  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Novel Mutation ◽  
Geographical Area ◽  
Encephalitis Virus ◽  
North India ◽  
Health Concern ◽  
Envelope Gene ◽  
Genotype Iii ◽  
Loop Region

SUMMARYJapanese encephalitis (JE) is an important arboviral infection of public health concern. There is a significant variation in mortality (10–30%) in JE viral infection. Epidemics of JE have become regular features in the northen states of India. The recent resurgence of the A226V mutation leading to a widespread Chikungunya epidemic motivated the investigators to search for any such mutational occurrence with Japanese encephalitis virus (JEV) isolated from this region. This study looked for mutation of clinical strains at amino-acid positions 176, 177, 227, 244, 264 and 279. A novel mutation S227T was detected corresponding to the loop region of domain II, E gene of JEV in comparison to Indian and other isolates from different parts of the world. Genotype III was found to be circulating in this geographical area. Further studies are required to ascertain its role in JE pathogenesis and vector competency.

scholarly journals Epidemiological Study of Japanese Encephalitis Virus in Vientiane, Lao PDR, in 1990s

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Mika Saito ◽  
Douangdao Soukaloun ◽  
Khampe Phongsavath ◽  
Bounlay Phommasack ◽  
Yoshihiro Makino
Keyword(s):  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Sequence Data ◽  
Lao Pdr ◽  
Laboratory Strain ◽  
Prototype Strain ◽  
Encephalitis Virus ◽  
Envelope Gene ◽  
Vietnamese Strain ◽  
Seroepidemiologic Study

Phylogenetic analysis of Japanese encephalitis virus (JEV) was conducted using core-premembrane and envelope gene sequence data of two strains from Vientiane, Lao People’s Democratic Republic, in 1993 and five from Okinawa, Japan, in 2002 and 2003, and previously published strains. The two Vientiane strains designated as LaVS56 and LaVS145 belonged to genotype 1 (G1) and the same subcluster of G1 as Australian strain in 2000, Thai strains in 1982–1985 and 2004-2005, and Vietnamese strain in 2005, but were distinct from the subcluster of recently distributing G1 strains widely in Asia including Okinawan strains and recent Lao strain in 2009. These clusters with own distinct distributions indicated involvements of different mechanisms and routes of spreading viruses and clarified that Australian G1 strain is from Southeast Asia, not from East Asia. Both Vientiane strains were antigenically close to P19-Br (G1, isolate, Thailand), but distinct from Nakayama (G3, prototype strain, Japan), Beijing-1 (G3, laboratory strain, China), and JaGAr#01 (G3, laboratory strain, Japan), demonstrated by cross-neutralization tests using polyclonal antisera. These results together with seroepidemiologic study conducted in Vientiane strongly suggest that diversified JEV cocirculated there in early 1990s.

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