The influence of 1-naphthaleneacetic acid and (2-chloroethyl)-trimethylammonium chloride on the carotenoid content of tobacco tissue in suspension culture

1978 ◽  
Vol 20 (2) ◽  
pp. 93-97 ◽  
Author(s):  
K. Z. Gamburg
Keyword(s):  
Suspension Culture ◽  
Carotenoid Content ◽  
Naphthaleneacetic Acid ◽  
Trimethylammonium Chloride

Phenolics from Cell Suspension Cultures of Penstemon serrulatus; Relation to Plant Organs

1997 ◽  
Vol 52 (7-8) ◽  
pp. 426-432 ◽  
Author(s):  
Zuzanna Skrzypek ◽  
Halina Wysokińska
Keyword(s):  
Cell Culture ◽  
Phenolic Compounds ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cultured Cells ◽  
Cell Suspension Cultures ◽  
Naphthaleneacetic Acid ◽  
Phenolic Composition ◽  
Phenylpropanoid Glycosides ◽  
Selection Of

Abstract By repeated selection of pigment portions of tissue the red callus induced from root seed­lings of Penstemon serrulatus Menz. was chosen for suspension culture, which was maintained in Schenk and Hildebrandt medium supplemented with naphthaleneacetic acid (0.2 mg/l), 6-benzylaminopurine (2 mg/l) and sucrose (50 g/l). From the cultured cells eight phenolic compounds were isolated. They were identified as cyanidin 3-O-glucoside, delphinidin 3-O-glucoside, luteolin, luteolin 7-O-glucoside, norartocarpetin 7-O-glucoside, verbascoside, martynoside and leucosceptoside A. The kind of cell line, its age and light irradiation were important factors in flavonoid production, but production of phenylpropanoid glycosides was found to be unaffected by these factors. The phenolic composition found in the cell culture was compared with those in the flowers and leaves of original plants of P. serrulatus.

scholarly journals Rapid Regeneration of Lettuce from Suspension Culture

HortScience ◽  
1992 ◽  
Vol 27 (9) ◽  
pp. 1030-1032 ◽  
Author(s):  
Whei-Lan Teng ◽  
Yann-Jiun Liu ◽  
Tai-Sen Soong
Keyword(s):  
Acetic Acid ◽  
Light Intensity ◽  
Suspension Culture ◽  
Basal Medium ◽  
Cell Suspensions ◽  
Naphthaleneacetic Acid ◽  
Cell Aggregates ◽  
Commercial Cultivars ◽  
Cell Growth And Differentiation ◽  
Optimal Medium

An efficient method for the regeneration of shoots directly from cell suspensions of three commercial cultivars of lettuce (Lactuca sativa L. cv. Great Lakes 659-700, Salad Bowl, and Prize Head) is described. Cell suspensions were prepared by osterizing cotyledon-derived callus for 60 seconds. The effects of callus quality, light intensity, carbohydrate type and concentration, auxins, and cytokinins on cell growth and differentiation in the suspension culture were examined. Among these factors, callus quality and carbohydrates were the most critical. The optimal medium for regeneration of shoots in suspension culture was SH (Schenk and Hilderbrandt) basal medium containing 1000 mg myo -inositol/liter, 1.5% glucose, 0.44 μm BA, and 0.54 μm NAA. The pH of the medium was adjusted to 5.8. Under such condition, hundreds of shoots could be produced from 50 to 55 mg (dry wt) of cell aggregates within 2 weeks. Chemical names used: α-] naphthaleneacetic acid (NAA); indole3-acetic acid (IAA); 6-benzylaminopurine (BA).

scholarly journals Alkaloids of in vitro biomass of papaver nudicaule l

2018 ◽  
Vol 25 (03) ◽  
pp. 90-96
Author(s):  
Otgonpurev S ◽  
Munguntsooj B ◽  
Ariunjargal B ◽  
Munkhtsetseg D
Keyword(s):  
Thin Layer ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Basal Medium ◽  
Naphthaleneacetic Acid ◽  
Proliferative Capacity ◽  
Whole Plant ◽  
Murashige Skoog ◽  
Layer Chromatography

Papaver nudicaule  L has a long history as a being useful for the treatment of many diseases in Asian and European countries. Aim of this study was to cultivate callus and cell suspension culture in vitro using plant phytohormones. The proliferative capacity was tested on shoot cultivated on Murashige-Skoog (MS) basal medium testing auxins: -Naphthaleneacetic acid (NAA)  in combination with cytokinine: 6-Benzylaminopurine. We determined production of alkaloids by four-week old callus and cell suspension biomass of Papaver nudicaule using thin layer chromatography comparing standard sanguianarine. This result displayed us the easier approach to isolate pure one alkaloid from the biomass that those whole- plant. Нүцгэн намуу ургамлын in vitro биомасс дахь алкалоид Хураангуй: Нүцгэн намуу (Papaver nudicaule) нь эмийн болон чимэглэлийн ач холбогдолтой ургамал юм. Уг ургамлын үрээс каллусын болон эсийн суспензийн биомассыг гарган авахдаа нафтален цууны хүчил болон бензил аденин өсөлтийн бодисын хослол бүхий тэжээлт орчинд өсгөвөрлөн өсөлтийн параметрүүдийг тогтоолоо. Ургамлын in vitro биомасст нимгэн үеийн хроматографийн аргаар сангвинарин алкалоидыг стандарт бодистой харьцуулан тодорхойлов. Сангвинарин нь ургамлын эд эрхтэний төрөлжилтөөс үл хамааран үүсдэг биологийн өндөр идэвхтэй хоёрдогч нийлэгжилтийн бүтээгдэхүүн юм. Түлхүүр үг: өсөлтийн гормоны хослол, каллусын эд, суспензийн нягт, нимгэн үеийн хроматографи

scholarly journals Establishment of a Cell Suspension Culture of Ageratina pichinchensis (Kunth) for the Improved Production of Anti-Inflammatory Compounds

Plants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1398
Author(s):  
Mariana Sánchez-Ramos ◽  
Laura Alvarez ◽  
Antonio Romero-Estrada ◽  
Antonio Bernabé-Antonio ◽  
Silvia Marquina-Bahena ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Ethyl Acetate ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Phytochemical Analysis ◽  
Naphthaleneacetic Acid ◽  
No Production ◽  
Phytochemical Study ◽  
A Cell ◽  
Anti Inflammatory

Ageratina pichinchensis (Kunth) is a plant used in traditional Mexican medicine to treat multiple ailments. However, there have not been biotechnological studies on producing compounds in in vitro cultures. The aim of this study was to establish a cell suspension culture of A. pichinchensis, quantify the anti-inflammatory constituents 2,3-dihydrobenzofuran (2) and 3-epilupeol (3), evaluate the anti-inflammatory potential of its extracts, and perform a phytochemical analysis. Cell suspension cultures were established in a MS culture medium of 30-g L−1 sucrose, 1.0-mg L−1 α-naphthaleneacetic acid, and 0.1-mg L−1 6-furfurylaminopurine. The ethyl acetate extract of the cell culture analyzed by gas chromatography (GC) revealed that the maximum production of anti-inflammatory compounds 2 and 3 occurs on days eight and 16, respectively, improving the time and previously reported yields in callus cultures. The anti-inflammatory activity of these extracts exhibited a significant inhibition of nitric oxide (NO) production. Furthermore, a phytochemical study of the ethyl acetate (EtOAc) and methanol (MeOH) extracts from day 20 led to the identification of 17 known compounds. The structures of the compounds were assigned by an analysis of 1D and 2D NMR data and the remainder by GC–MS. This is the first report of the production of (-)-Artemesinol, (-)-Artemesinol glucoside, encecalin, and 3,5-diprenyl-acetophenone by a cell suspension culture of A. pichinchensis.

Carotenoid content of the flower of tansy (Tanacetum vulgare L.)

Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
G Horváth ◽  
E Turcsi ◽  
P Molnár ◽  
LG Szabó ◽  
J Deli
Keyword(s):  
Carotenoid Content ◽  
Tanacetum Vulgare

ВПЛИВ ДЖЕРЕЛ АЗОТНОГО ЖИВЛЕННЯ НА СИНТЕЗ КАРОТИНОЇДІВ ДЕЯКИМИ ШТАМАМИ БАЗИДІОМІЦЕТІВ

2014 ◽  
Vol 4 (01) ◽  
pp. 22
Author(s):  
A. K. Veligodska ◽  
O. V. Fedotov ◽  
A. S. Petreeva
Keyword(s):  
Culture Filtrate ◽  
Nutrient Medium ◽  
Culture Fluid ◽  
Carotenoid Content ◽  
Nitrogen Compounds ◽  
Total Carotenoid ◽  
Carotenoid Accumulation ◽  
Total Carotenoid Content ◽  
Peptone Medium ◽  
Acetone Extracts

<p>The influence of certain nitrogen compounds - components of glucose-peptone medium (GPM) on the accumulation of carotenoids by some strains was investigated by surface cultivating basidiomycetes. The total carotenoid content was set in acetone extracts of mycological material spectrophotometrically and calculated using the Vetshteyn formula.</p> <p>As the nitrogen-containing components used GPM with 9 compounds, such as peptone, DL-valine, L-asparagine, DL-serine, DL-tyrosine, L-proline, L-alanine, urea, NaNO<sub>3</sub>. The effect on the accumulation of specific compounds both in the mycelium and in the culture fluid of carotenoids by culturing certain strains of Basidiomycetes was identified.</p> <p>Adding to standard glucose-peptone medium peptone at 5 g/l causes an increase of carotenoid accumulation by strain <em>L. sulphureus</em> Ls-08, and in a concentration of 4 g/l by strains of <em>F. hepatica </em>Fh-18 and <em>F. fomentarius</em> Ff-1201.</p> <p>In order to increase the accumulation of carotenoids in the mycelium  we suggested to make a standard glucose-peptone medium with proline or valine for cultivating of <em>L. sulphureus</em> Ls- 08 strain; alanine for <em>F. fomentarius</em> Ff-1201 strain; proline, asparagine and serine - for strain Fh-18 of <em>F. hepatica</em>. The results can be implemented in further optimization of the composition of the nutrient medium for culturing strains of Basidiomycetes wich producing carotenoids.</p> <p><em>Keywords: nitrogen-containing substances, Basidiomycetes, mycelium</em><em>,</em><em> culture filtrate, carotenoids</em></p>

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