Nitrate reductase, nitrite reductase and glutamate dehydrogenase levels in roots and leaves of maize seedlings

1976 ◽  
Vol 18 (4) ◽  
pp. 283-289 ◽  
Author(s):  
Tatiana Pšenáková ◽  
Otília Gašparíková ◽  
Anna Nižňanská
Keyword(s):  
Nitrate Reductase ◽  
Glutamate Dehydrogenase ◽  
Nitrite Reductase ◽  
Maize Seedlings

scholarly journals The regulation of activity of the enzymes involved in the assimilation of nitrate by higher plants

1966 ◽  
Vol 100 (3) ◽  
pp. 577-588 ◽  
Author(s):  
J Ingle ◽  
K W Joy ◽  
R H Hageman
Keyword(s):  
Nitrate Reductase ◽  
Glutamate Dehydrogenase ◽  
Nitrite Reductase ◽  
Actinomycin D ◽  
Higher Plants ◽  
Specific Inhibitor ◽  
Nitrate Assimilation ◽  
End Products ◽  
Regulation Of Activity

1. Possible mechanisms regulating the activities of three enzymes involved in nitrate assimilation, nitrate reductase, nitrite reductase and glutamate dehydrogenase, were studied in radish cotyledons. 2. Nitrate-reductase and nitrite-reductase activities are low in nitrogen-deficient cotyledons, and are induced by their substrates. 3. Glutamate dehydrogenase is present regardless of the nitrogen status, and the enzyme can be increased only slightly by long-term growth on ammonia. 4. Although nitrate is the best inducer of nitrate reductase, lower levels of induction are also obtained with nitrite and ammonia. The experiments did not distinguish between direct or indirect induction by these two molecules. 5. Nitrite reductase is induced by nitrite and only indirectly by nitrate. 6. The induction of both nitrate reductase and nitrite reductase is prevented by the inhibitors actinomycin D, puromycin and cycloheximide, indicating a requirement for the synthesis of RNA and protein. 7. The decay of nitrate reductase, determined after inhibition of protein synthesis, is slower than the synthesis of the enzyme. Nitrite reductase is much more stable than nitrate reductase. 8. The synthesis of nitrate reductase is not repressed by ammonia, but is repressed by growth on a nitrite medium. 9. There is no inhibition of nitrate reductase, nitrite reductase or glutamate dehydrogenase by the normal end products of assimilation, but cyanate is a fairly specific inhibitor of nitrate reductase.

Effect of glutamine supply on nitrate reductase isoforms in maize seedlings

1998 ◽  
Vol 47 (5) ◽  
pp. 701-706 ◽  
Author(s):  
Niharika Shankar ◽  
Hari S. Srivastava
Keyword(s):  
Nitrate Reductase ◽  
Maize Seedlings

scholarly journals Frequencies, Timing, and Spatial Patterns of Co-Suppression of Nitrate Reductase and Nitrite Reductase in Transgenic Tobacco Plants

1996 ◽  
Vol 112 (4) ◽  
pp. 1447-1456 ◽  
Author(s):  
J. C. Palauqui ◽  
T. Elmayan ◽  
F. D. de Borne ◽  
P. Crete ◽  
C. Charles ◽  
...  
Keyword(s):  
Nitrate Reductase ◽  
Transgenic Tobacco ◽  
Spatial Patterns ◽  
Nitrite Reductase ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants
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