Effect of gamma-radiation on the growth of primary and “long-term” tissue cultures (Nicotiana tabacum L.)

1974 ◽  
Vol 16 (6) ◽  
pp. 418-425 ◽  
Author(s):  
Z. Opatrný
Keyword(s):  
Nicotiana Tabacum ◽  
Gamma Radiation ◽  
Tissue Cultures ◽  
Nicotiana Tabacum L

Cytokinin autotrophy and differentiation in tissue cultures of haploid Nicotiana tabacum L.

Plant Science ◽  
1986 ◽  
Vol 45 (2) ◽  
pp. 125-132 ◽  
Author(s):  
Gilberto Barbante Kerbauy ◽  
JoséAntonio Peters ◽  
Kurt Gunther Hell
Keyword(s):  
Nicotiana Tabacum ◽  
Tissue Cultures ◽  
Nicotiana Tabacum L

scholarly journals Growth and Division of Single Cells of Higher Plants in Vitro

1960 ◽  
Vol 43 (4) ◽  
pp. 841-851 ◽  
Author(s):  
Ludwig Bergmann
Keyword(s):  
Nicotiana Tabacum ◽  
Higher Plants ◽  
Single Cells ◽  
Tissue Cultures ◽  
Phaseolus Vulgaris L ◽  
Isolated Cells ◽  
Nicotiana Tabacum L ◽  
Agar Layer ◽  
Petri Dishes

The cultivation of single cells of Nicotiana tabacum L. var. "Samsun" and Phaseolus vulgaris L. var. "Early Golden Cluster" on a thin agar layer in Petri dishes is described. Under these conditions about 20 per cent of the cells divided repeatedly and established tissue clones which could be isolated and maintained as growing tissue cultures. It was possible also to follow the successive divisions of isolated cells and to observe their behavior during cytogenesis under the microscope.

Establishment of stable long-term photomixotrophic cell cultures of tobacco (Nicotiana Tabacum L.)

Plant Science ◽  
1989 ◽  
Vol 61 (1) ◽  
pp. 145-151 ◽  
Author(s):  
Wen C. Wang ◽  
James R. Myers ◽  
Glenn B. Collins
Keyword(s):  
Nicotiana Tabacum ◽  
Cell Cultures ◽  
Nicotiana Tabacum L

Long‐term Genetic Improvement and Genetic Diversity of Croatian Flue‐cured Tobacco ( Nicotiana tabacum L.) Cultivars

Crop Science ◽  
2013 ◽  
Vol 53 (1) ◽  
pp. 112-120
Author(s):  
H. Šarčević ◽  
J. Gunjaća ◽  
A. Budimir ◽  
M. Boić ◽  
S. Bolarić ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Nicotiana Tabacum ◽  
Genetic Improvement ◽  
Nicotiana Tabacum L

scholarly journals Stimulation of Nicotiana tabacum L. In Vitro Shoot Growth by Endophytic Bacillus cereus Group Bacteria

2021 ◽  
Vol 9 (9) ◽  
pp. 1893
Author(s):  
Elena Andriūnaitė ◽  
Inga Tamošiūnė ◽  
Monika Aleksandravičiūtė ◽  
Dalia Gelvonauskienė ◽  
Jurgita Vinskienė ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Endophytic Bacteria ◽  
Shoot Growth ◽  
Tissue Cultures ◽  
Bacillus Cereus Group ◽  
Nicotiana Tabacum L ◽  
Bacillus Spp ◽  
Plant Tissue Cultures ◽  
In Vitro Plant

In vitro plant tissue cultures face various unfavorable conditions, such as mechanical damage, osmotic shock, and phytohormone imbalance, which can be detrimental to culture viability, growth efficiency, and genetic stability. Recent studies have revealed a presence of diverse endophytic bacteria, suggesting that engineering of the endophytic microbiome of in vitro plant tissues has the potential to improve their acclimatization and growth. Therefore, the aim of this study was to identify cultivated tobacco (Nicotiana tabacum L.) endophytic bacteria isolates that are capable of promoting the biomass accumulation of in vitro tobacco shoots. Forty-five endophytic bacteria isolates were obtained from greenhouse-grown tobacco plant leaves and were assigned to seven Bacillus spp. and one Pseudomonas sp. based on 16S rRNA or genome sequence data. To evaluate the bacterial effect on in vitro plant growth, tobacco shoots were inoculated with 22 isolates selected from distinct taxonomic groups. Four isolates of Bacillus cereus group species B. toyonensis, B. wiedmannii and B. mycoides promoted shoot growth by 11–21%. Furthermore, a contrasting effect on shoot growth was found among several isolates of the same species, suggesting the presence of strain-specific interaction with the plant host. Comparative analysis of genome assemblies was performed on the two closely related B. toyonensis isolates with contrasting plant growth-modulating properties. This revealed distinct structures of the genomic regions, including a putative enzyme cluster involved in the biosynthesis of linear azol(in)e-containing peptides and polysaccharides. However, the function of these clusters and their significance in plant-promoting activity remains elusive, and the observed contrasting effects on shoot growth are more likely to result from genomic sequence variations leading to differences in metabolic or gene expression activity. The Bacillus spp. isolates with shoot-growth-promoting properties have a potential application in improving the growth of plant tissue cultures in vitro.

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