Formation and purification of nitrile hydratase fromCorynebacterium pseudodiphteriticum ZBB-41

1992 ◽  
Vol 36 (3) ◽  
pp. 171-181 ◽  
Author(s):  
Wen-Zhong Li ◽  
Yi-Qiang Zhang ◽  
Hui-Fang Yang
Keyword(s):  
Nitrile Hydratase

The Stability Enhancement of Nitrile Hydratase from Bordetella petrii by Swapping the C-terminal Domain of β subunit

2015 ◽  
Vol 178 (8) ◽  
pp. 1481-1487 ◽  
Author(s):  
Weifeng Sun ◽  
Longbao Zhu ◽  
Xianggui Chen ◽  
Lunjie Wu ◽  
Zhemin Zhou ◽  
...  
Keyword(s):  
Nitrile Hydratase ◽  
Β Subunit ◽  
Terminal Domain ◽  
The Stability ◽  
Stability Enhancement

scholarly journals Transcriptional Regulation of the Nitrile Hydratase Gene Cluster in Pseudomonas chlororaphis B23

2008 ◽  
Vol 190 (12) ◽  
pp. 4210-4217 ◽  
Author(s):  
Toshihide Sakashita ◽  
Yoshiteru Hashimoto ◽  
Ken-Ichi Oinuma ◽  
Michihiko Kobayashi
Keyword(s):  
Gene Cluster ◽  
Nitrile Hydratase ◽  
Transcriptional Regulator ◽  
Pseudomonas Chlororaphis ◽  
Reading Frame ◽  
Transcription Start Sites ◽  
Reverse Transcription Pcr ◽  
Enormous Amount ◽  
Extension Analysis ◽  
Arac Family

ABSTRACT An enormous amount of nitrile hydratase (NHase) is inducibly produced by Pseudomonas chlororaphis B23 after addition of methacrylamide as the sole nitrogen source to a medium. The expression pattern of the P. chlororaphis B23 NHase gene cluster in response to addition of methacrylamide to the medium was investigated. Recently, we reported that the NHase gene cluster comprises seven genes (oxdA, amiA, nhpA, nhpB, nhpC, nhpS, and acsA). Sequence analysis of the 1.5-kb region upstream of the oxdA gene revealed the presence of a 936-bp open reading frame (designated nhpR), which should encode a protein with a molecular mass of 35,098. The deduced amino acid sequence of the nhpR product showed similarity to the sequences of transcriptional regulators belonging to the XylS/AraC family. Although the transcription of the eight genes (nhpR, oxdA, amiA, nhpABC, nhpS, and acsA) in the NHase gene cluster was induced significantly in the P. chlororaphis B23 wild-type strain after addition of methacrylamide to the medium, transcription of these genes in the nhpR disruptant was not induced, demonstrating that nhpR codes for a positive transcriptional regulator in the NHase gene cluster. A reverse transcription-PCR experiment revealed that five genes (oxdA, amiA, nhpA, nhpB, and nhpC) are cotranscribed, as are two other genes (nhpS and acsA). The transcription start sites for nhpR, oxdA, nhpA, and nhpS were mapped by primer extension analysis, and putative −12 and −24 σ54-type promoter binding sites were identified. NhpR was found to be the first transcriptional regulator of NHase belonging to the XylS/AraC family.

scholarly journals Inhibitor binding of nitrile hydratase fromRhodococcus sp. N771 in the photoactivation process

2002 ◽  
Vol 58 (s1) ◽  
pp. c119-c119
Author(s):  
M. Nojiri ◽  
Y. Kawano ◽  
M. Odaka ◽  
H. Nakajima ◽  
I. Endo ◽  
...  
Keyword(s):  
Nitrile Hydratase ◽  
Inhibitor Binding
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