Expression of foreign genes in mammalian cells using an antibody fusion system

1994 ◽  
Vol 1 (3) ◽  
pp. 251-263
Author(s):  
Simon J. Forster ◽  
Francis J. Carr ◽  
William J. Harris ◽  
Anita A. Hamilton
Keyword(s):  
Mammalian Cells ◽  
Fusion System ◽  
Foreign Genes

Expression of Foreign Genes in Mammalian Cells Using an Antibody Fusion System

2003 ◽  
pp. 461-474
Author(s):  
Simon J. Forster ◽  
Francis J. Carr ◽  
William J. Harris ◽  
Anita A. Hamilton
Keyword(s):  
Mammalian Cells ◽  
Fusion System ◽  
Foreign Genes

scholarly journals A novel BK virus-based episomal vector for expression of foreign genes in mammalian cells

1991 ◽  
Vol 19 (8) ◽  
pp. 1925-1931 ◽  
Author(s):  
Arrigo De Benedetti ◽  
Robert E. Rhoads
Keyword(s):  
Mammalian Cells ◽  
Bk Virus ◽  
Episomal Vector ◽  
Foreign Genes

scholarly journals Improved vectors for stable expression of foreign genes in mammalian cells by use of the untranslated leader sequence from EMC virus

1991 ◽  
Vol 19 (16) ◽  
pp. 4485-4490 ◽  
Author(s):  
Randal J. Kaufman ◽  
Monique V. Davies ◽  
Louise C. Wasley ◽  
Donna Michnick
Keyword(s):  
Mammalian Cells ◽  
Leader Sequence ◽  
Stable Expression ◽  
Emc Virus ◽  
Foreign Genes

Mulcos: a vector for amplification and simultaneous expression of two foreign genes in mammalian cells

Gene ◽  
1988 ◽  
Vol 71 (1) ◽  
pp. 19-27 ◽  
Author(s):  
Ikeda Hitoshi ◽  
Trowsdale John ◽  
Saito Izumu
Keyword(s):  
Mammalian Cells ◽  
Foreign Genes ◽  
Simultaneous Expression

scholarly journals Expression of genes coding for animal virus glycoproteins in heterologous systems.

1999 ◽  
Vol 46 (2) ◽  
pp. 325-339 ◽  
Author(s):  
K Bieńkowska-Szewczyk ◽  
B Szewczyk
Keyword(s):  
Mammalian Cells ◽  
Insect Cells ◽  
Host Cells ◽  
Animal Virus ◽  
Expression Of Genes ◽  
Viral Glycoproteins ◽  
Foreign Genes ◽  
Nuclear Polyhedrosis ◽  
High Level ◽  
Expression In Mammalian Cells

The outermost layers of animal viruses are usually composed of glycoproteins. They are responsible not only for the entrance of viruses into, and release from host cells but also for the initial interaction of a viral particle with immunological defense of the host. It is therefore not surprising that many laboratories devote a lot of effort to study viral glycoproteins at the molecular level. Very often such studies are possible only after the introduction of a glycoprotein gene into a heterologous system. Expression of glycoprotein genes is usually obtained in mammalian or insect cells. Expression in mammalian cells yields viral glycoproteins with glycan chains indistinguishable from the original counterparts in virion particles but the level of synthesis of glycoproteins is very low. Vaccinia virus is the most common vector for expression in mammalian cells. It is easy to grow, the introduction of foreign genes is relatively simple and, due to the size of the vaccinia genome, it can accept large pieces of foreign DNA. Glycosylation in insect cells is not as complex as in mammalian cells and usually glycoproteins produced in insect cells are of slightly lower molecular mass than those produced in mammalian cells. The most common vector for expression of glycoproteins in insect cells is a baculovirus, Autographa californica nuclear polyhedrosis virus (AcNPV). The great advantage of this system is a very high level of expression of foreign genes.

High efficiency gene transfer into mammalian cells

1984 ◽  
Vol 307 (1132) ◽  
pp. 343-346 ◽  
Keyword(s):  
Gene Transfer ◽  
Mammalian Cells ◽  
High Efficiency ◽  
Single Copy ◽  
Rapid Screening ◽  
Embryonic Cells ◽  
Copy Gene ◽  
Foreign Dna ◽  
Foreign Genes ◽  
Transient System

We have generalized the protocol of gene transfer, greatly increasing the variety of cells that can be used as recipients of foreign genes. Our approach has been to use a transient assay system that allows rapid screening of expression of foreign DNA. When the initial steps of gene transfer have been optimized with the transient system, these defined conditions are used to yield efficient stable transformation. We have seen that primate cells, including human cells, can be used in gene transfer experiments at levels sensitive enough to allow detection of single copy gene function. Recently we have also used this approach successfully with undifferentiated embryonic cells.

scholarly journals Regulated expression of foreign genes in mammalian cells under the control of coliphage T3 RNA polymerase and lac repressor.

1989 ◽  
Vol 86 (14) ◽  
pp. 5400-5404 ◽  
Author(s):  
U. Deuschle ◽  
R. Pepperkok ◽  
F. B. Wang ◽  
T. J. Giordano ◽  
W. T. McAllister ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Mammalian Cells ◽  
Lac Repressor ◽  
Regulated Expression ◽  
Foreign Genes
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