Resistance to ACNU induced toxicity in transgenic tobacco suspension cultures withada gene transferred fromEscherichia coli

1993 ◽  
Vol 35 (1) ◽  
pp. 125-129 ◽  
Author(s):  
J. Briza ◽  
K. J. Angelis ◽  
J. Satava ◽  
I. Baburek ◽  
J. Veleminsky
Keyword(s):  
Transgenic Tobacco ◽  
Suspension Cultures ◽  
Fromescherichia Coli ◽  
Tobacco Suspension Cultures

scholarly journals Rapid, Cf-9- and Avr9-Dependent Production of Active Oxygen Species in Tobacco Suspension Cultures

1998 ◽  
Vol 11 (12) ◽  
pp. 1155-1166 ◽  
Author(s):  
Pedro Piedras ◽  
Kim E. Hammond-Kosack ◽  
Kate Harrison ◽  
Jonathan D. G. Jones
Keyword(s):  
Transgenic Tobacco ◽  
Resistance Gene ◽  
Gene Function ◽  
Active Oxygen Species ◽  
Defense Responses ◽  
Suspension Cultures ◽  
Active Oxygen ◽  
Avirulence Gene ◽  
Oxygen Species ◽  
Tobacco Suspension Cultures

The tomato Cf-9 gene confers resistance to races of Cla-dosporium fulvum expressing the corresponding avirulence gene Avr9. The availability of transgenic tobacco lines carrying Cf-9, and the well-characterized 28 amino acid Avr9 elicitor, make this an excellent system to study resistance gene function. In this paper we establish tobacco suspension cultures derived from transgenic tobacco plants containing Cf-9, as well as from plants without Cf-9. Cultures derived from Cf9 tobacco produce active oxygen species (AOS) within 5 min of treatment with pure or synthetic Avr9. This enabled us to perform biochemical and pharmacological analysis in cell culture of the very earliest events in resistance gene function. In addition to AOS production, an increase in oxygen uptake was detected in the Avr9-treated Cf9 cells. Both phenomena were inhibited by low concentrations of diphenyleneiodonium (DPI). Additional pharmacological inhibitor studies suggest that uptake of calcium, activation of protein kinases, and probably phospholipase A2 (PLA2) activity are intermediates in the Cf-9- and Avr9-dependent signaling pathway that leads to AOS production. Interestingly, those defense responses did not result in plant cell death.

Evidence for the Participation of a 5-Oxo-prolinase in Degradation of Glutathione in Nicotiana tabacum

1980 ◽  
Vol 35 (9-10) ◽  
pp. 708-711 ◽  
Author(s):  
Heinz Rennenberg ◽  
Reinhard Steinkamp ◽  
Andrea Polle
Keyword(s):  
Nicotiana Tabacum ◽  
Glutamic Acid ◽  
Suspension Cultures ◽  
Sulfur Source ◽  
Tobacco Cells ◽  
Tobacco Suspension Cultures

Abstract During degradation of glutathione in tobacco suspension cultures substancial amounts of 5-oxo-proline are formed in vivo as well as in crude cell homogenates in vitro. The existance of a 5-oxo-prolinase that catalyzes the conversion of 5-oxo-proline to glutamic acid was demonstrated in tobacco cells, grown with glutathione as sole sulfur source.

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