The influence of physical properties on the operation of a three-phase fluidized-bed fermentor with yeast cells immobilized in Ca-alginate

1992 ◽  
Vol 34-35 (1) ◽  
pp. 467-475 ◽  
Author(s):  
P. Béjar ◽  
C. Casas ◽  
F. Gödia ◽  
C. Solà
Author(s):  
Boutros F Sarrouh ◽  
Silvio Silvério da Silva

This work deals with the evaluation of the hydrodynamic characteristics and fermentation parameters of a bench-scale three-phase fluidized bed reactor (FBR) with cells of the yeast Candida guilliermondii immobilized calcium alginate beads for xylitol production. The effects of the following hydrodynamic variables on cell immobilization were evaluated: total particle density, terminal velocity, particles drag force, minimum fluidization velocity and bed porosity (?). According to the results obtained, the reactor was shown to operate similar to a fixed-bed bioreactor at ? < 0.5 and a fluidized bed bioreactor at ? > 0.5, respectively. The maximum flowrate needed to obtain maximum bed fluidization in the reactor was equal to the terminal velocity of the immobilized cell particles. As for the fermentation parameters, the effects of the aeration rate and fermentation time on the process productivity and yield were analyzed. The maximum xylitol concentration of 28.9 g xylitol/l was obtained at a high aeration rate of 600ml/min after 70h of fermentation, indicating that the use of a high aeration rate in this system is favored for better oxygen transfer into the immobilized cells. The specific xylitol productivity and the xylitol yield were of 0.41 g xylitol/l.h and 0.582 g xylitol/g xylose respectively. After 90h of fermentation xylitol productivity and yield decreased to 0.256 g xylitol/l.h and 0.47 g xylitol/g xylose respectively, indicating the beginning of xylitol consumption by the yeast.


2009 ◽  
Vol 33 (1) ◽  
pp. 359-370 ◽  
Author(s):  
Mauren Fuentes ◽  
Miguel C. Mussati ◽  
Nicolás J. Scenna ◽  
Pío A. Aguirre

2000 ◽  
Vol 33 (3) ◽  
pp. 365-371 ◽  
Author(s):  
Tsutomu Tashimo ◽  
Jun Murota ◽  
Tomohiko Suto ◽  
Kunio Kato

2018 ◽  
Vol 61 (3) ◽  
pp. 269-285 ◽  
Author(s):  
R. K. Padhi ◽  
D. T. K. Dora ◽  
Y. K. Mohanty ◽  
G. K. Roy ◽  
B. Sarangi

1991 ◽  
Vol 23 (7-9) ◽  
pp. 1347-1354 ◽  
Author(s):  
F. Trinet ◽  
R. Heim ◽  
D. Amar ◽  
H. T. Chang ◽  
B. E. Rittmann

A three-phase, liquid-fluidized-bed biofilm reactor was operated over wide ranges of liquid velocity, air velocity, medium concentration, and substrate surface loading. The biofilm characteristics (total colonization, polysaccharide content, density, and thickness) and the specific detachment coefficient (bs) were determined by a combination of experimental measurements and a hydrodynamic model. The results demonstrated that dense and thin biofilms were induced by the physical condition of high particle-to-particle contacts and high liquid turbulence. The biofilm's polysaccharide content was increased by increased air turbulence and a low substrate availability. The specific detachment coefficient, bs, was strongly correlated to the concentration of the medium (negatively) and the polysaccharide content (positively). Overall, the bs can be controlled significantly by the gas and liquid velocities; increasing either velocity tends to increase bs.


2019 ◽  
Vol 209 ◽  
pp. 115177 ◽  
Author(s):  
Keying Ma ◽  
Xiliang Sun ◽  
Yuanyuan Shao ◽  
Mingyan Liu ◽  
Jesse Zhu

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