Soluble inhibitors of T lymphocyte proliferation: Tools for dissecting pathways of T cell activation

1988 ◽  
Vol 7 (2) ◽  
pp. 93-112 ◽  
Author(s):  
John C. Reed ◽  
Peter C. Nowell
Keyword(s):  
T Cell ◽  
T Cell Activation ◽  
T Lymphocyte ◽  
Lymphocyte Proliferation ◽  
Cell Activation ◽  
T Lymphocyte Proliferation

RecombinantPseudomonasexoenzyme S and exoenzyme S fromPseudomonas aeruginosaDG1 share the ability to stimulate T lymphocyte proliferation

1999 ◽  
Vol 45 (7) ◽  
pp. 607-611 ◽  
Author(s):  
Tony F Bruno ◽  
Donald E Woods ◽  
Douglas G Storey ◽  
Christopher H Mody
Keyword(s):  
Pseudomonas Aeruginosa ◽  
T Cell ◽  
T Cell Activation ◽  
T Lymphocyte ◽  
Lymphocyte Proliferation ◽  
Cell Activation ◽  
Lymphocyte Activation ◽  
Activation Markers ◽  
Exoenzyme S ◽  
T Lymphocyte Proliferation

Exoenzyme S from P. aeruginosa DG1 and recombinant exoenzyme S derived from strain 388 have distinct characteristics, which has led to a controversy about their homology and their pathophysiologic consequences. We have been investigating the ability of exoenzyme S to activate T lymphocytes, and therefore performed studies to determine whether exoenzyme S from P. aeruginosa DG1 and recombinant exoenzyme S derived from strain 388 and expressed in Pseudomonas aeruginosa PA103 or in E. coli BL21(DE3), could induce T lymphocyte activation and proliferation. Both preparations were able to activate T cells and induce lymphocyte proliferation at similar levels as measured by flow cytometry of surface-activation markers and DNA synthesis, respectively. Further, a monoclonal antibody raised against exoenzyme S from strain DG1 partially neutralized T cell activation induced by recombinant exoenzyme S and bound to it in an immunoblot suggesting that the epitope responsible for T cell activation is shared by exoenzyme S from strain DG1 and recombinant exoenzyme S. These data suggest that the two different preparations of exoenzyme S, despite biochemical differences, share the characteristic that is responsible for T lymphocyte activation.Key words: exoenzyme S, Pseudomonas aeruginosa, T lymphocyte, cystic fibrosis.

Immunomodulatory activity ofLactobacillusstrains isolated from fermented vegetables and infant stool

2011 ◽  
Vol 89 (6) ◽  
pp. 429-434 ◽  
Author(s):  
Tae Joon Won ◽  
Bongjoon Kim ◽  
Eun Seul Oh ◽  
Joon Seok Bang ◽  
Yoon Jeong Lee ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Activation ◽  
T Lymphocyte ◽  
Lymphocyte Proliferation ◽  
Cell Activation ◽  
B Lymphocyte ◽  
Fermented Food ◽  
Immunomodulatory Activity ◽  
Lymphocyte Population ◽  
T Lymphocyte Proliferation

Four Lactobacillus strains — Lactobacillus plantarum CJLP133, L. plantarum CJLP243, L. plantarum CJNR26, and Lactobacillus gasseri CJMF3 — were isolated from Korean fermented food or healthy infant feces, and their capacity to modulate cellular and humoral immune responses was studied. Feeding of the tested lactobacilli for 8 weeks did not alter the weight of and cell numbers in the spleen of mice. However, CJLP133 and CJLP243 strains increased the T lymphocyte population in the spleen of mice, while CJNR26 and CJMF3 increased the B lymphocyte population. In splenocytes treated with concanavalin A, ingestion of CJLP133 and CJLP243 promoted T lymphocyte proliferation and secretion of T cell cytokines, whereas feeding of the CJNR26 and CJMF3 strains enhanced B lymphocyte proliferation in splenocytes treated with lipopolysaccharide and plaque formation. These results suggest that CJLP133 and CJLP243 have immunostimulating activity through the enhancement of T cell activation, while CJNR26 and CJMF3 exhibit immunopotentiation through the increment of B cell activation.

scholarly journals Simultaneous inhibition of human CD4 and 4-1BB biogenesis suppresses cytotoxic T lymphocyte proliferation

2021 ◽  
Author(s):  
Elisa Claeys ◽  
Eva Pauwels ◽  
Stephanie Humblet-Baron ◽  
Dominique Schols ◽  
Mark Waer ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Cell Activation ◽  
Lymphocyte Proliferation ◽  
Cell Activation ◽  
Protein Biosynthesis ◽  
Cell Subpopulation ◽  
T Lymphocyte Proliferation

ABSTRACTThe small molecule cyclotriazadisulfonamide (CADA) down-modulates the human CD4 receptor, an important factor in T cell activation. Here, we addressed the immunosuppressive potential of CADA using in vitro activation models. CADA inhibited lymphocyte proliferation in a mixed lymphocyte reaction, and when human PBMCs were stimulated with CD3/CD28 beads or phytohemagglutinin. The immunosuppressive effect of CADA involved both CD4+ and CD8+ T cells but was, surprisingly, most prominent in the CD8+ T cell subpopulation where it inhibited cell-mediated lympholysis. We discovered a direct down-modulatory effect of CADA on 4-1BB (CD137) expression, a survival factor for activated CD8+ T cells. More specifically, CADA blocked 4-1BB protein biosynthesis by inhibition of its co-translational translocation across the ER membrane in a signal peptide-dependent way. This study demonstrates that CADA, as potent down-modulator of human CD4 and 4-1BB, has promising in vitro immunomodulatory characteristics for future in vivo exploration as immunosuppressive drug.

scholarly journals Early Immune Markers Associated withMycobacterium aviumsubsp.paratuberculosisInfection in a Neonatal Calf Model

2011 ◽  
Vol 18 (3) ◽  
pp. 393-405 ◽  
Author(s):  
J. R. Stabel ◽  
S. Robbe-Austerman
Keyword(s):  
T Cell ◽  
T Cell Activation ◽  
Lymphocyte Proliferation ◽  
Cell Activation ◽  
The Other ◽  
T Cell Subsets ◽  
Cell Mediated Immunity ◽  
Activation Markers ◽  
Oral Inoculation ◽  
Ifn Γ

ABSTRACTThe objective of this study was to observe early markers of cell-mediated immunity in naïve calves infected withMycobacterium aviumsubsp.paratuberculosisand how expression of these markers evolved over the 12-month period of infection. Groups for experimental infection included control (noninfected), oral (infected orally withM. aviumsubsp.paratuberculosisstrain K-10), oral/DXM (pretreatment with dexamethasone before oral inoculation), intraperitoneal (i.p.) inoculation, and oral/M (oral inoculation with mucosal scrapings from a cow with clinical disease) groups. One of the earliest markers to emerge was antigen-specific gamma interferon (IFN-γ). Only i.p. inoculated calves had detectable antigen-specific IFN-γ responses at 7 days, with responses of the other infection groups becoming detectable at 90 and 120 days. All infection groups maintained robust IFN-γ responses for the remainder of the study. At 1 month, calves in the oral and oral/M groups had higher antigen-stimulated interleukin-10 (IL-10) levels than calves in the other treatment groups, but IL-10 secretion declined by 12 months for all calves. T-cell activation markers such as CD25, CD26, CD45RO, and CD5 were significantly upregulated in infected calves compared to noninfected controls. Oral inoculation of calves resulted in significantly increased antigen-specific lymphocyte proliferation at 9 and 12 months, as well as inducible nitric oxide synthase (iNOS) secretion at 6 and 12 months. These results demonstrate that infection of naïve calves withM. aviumsubsp.paratuberculosisinvoked early immunologic responses characterized by robust antigen-specific IFN-γ responses and induction of CD25 and CD45RO expression on T-cell subsets. These were followed by antigen-specific lymphocyte proliferation, iNOS secretion, and expression of CD26 and CD5brightmarkers in the latter part of the 12-month study.

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