Selenium retention and inhibition of cell growth in mouse mammary epithelial cell lines in vitro

1985 ◽  
Vol 8 (1) ◽  
pp. 19-35 ◽  
Author(s):  
Daniel Medina ◽  
David Morrison ◽  
Carol J. Oborn
Keyword(s):  
Cell Growth ◽  
Epithelial Cell ◽  
Cell Lines ◽  
Mammary Epithelial Cell ◽  
Mammary Epithelial ◽  
Mouse Mammary Epithelial Cell ◽  
Epithelial Cell Lines

scholarly journals In vitroeffects of lactoferrin on intestinal and mammary epithelial cell lines

2009 ◽  
Vol 8 (sup2) ◽  
pp. 643-645 ◽  
Author(s):  
Chiara Pecorini ◽  
Raffaella Rebucci ◽  
Sandrine Truchet ◽  
Antonella Baldi
Keyword(s):  
Epithelial Cell ◽  
Cell Lines ◽  
Mammary Epithelial Cell ◽  
Mammary Epithelial ◽  
Epithelial Cell Lines

MicroRNA-212 targets SIRT2 to influence lipogenesis in bovine mammary epithelial cell line

2020 ◽  
Vol 87 (2) ◽  
pp. 232-238
Author(s):  
Xubin Lu ◽  
Hailei Xia ◽  
Jingyi Jiang ◽  
Xin Xu ◽  
Mingxun Li ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Lines ◽  
Mammary Epithelial Cell ◽  
Regulatory Element ◽  
Fatty Acid Synthetase ◽  
Mammary Epithelial ◽  
Epithelial Cell Line ◽  
Peroxisome Proliferator ◽  
Epithelial Cell Lines ◽  
Verification Techniques

AbstractIn this research paper we filter and verify miRNAs which may target silent information regulator homolog 2 (SIRT2) gene and then describe the mechanism whereby miRNA-212 might regulate lipogenic genes in mammary epithelial cell lines via targeting SIRT2. Bioinformatics analysis revealed that the bovine SIRT2 gene is regulated by three miRNAs: miR-212, miR-375 and miR-655. The three miRNAs were verified and screened by qRT-PCR, western blot, and luciferase multiplex verification techniques and only miR-212 was shown to have a targeting relationship with SIRT2. The results of co-transfecting miR-212 and silencing RNA (siRNA) showed that by targeting SIRT2, miR-212 can regulate the expression of fatty acid synthetase (FASN) and sterol regulatory element binding factor 1 (SREBP1) but not peroxisome proliferator-activated receptor gamma (PPARγ). Measurement of triglyceride (TAG) content showed that miR-212 increased the fat content of mammary epithelial cell lines. The study indicates that miR-212 could target and inhibit the expression of the SIRT2 gene to promote lipogenesis in mammary epithelial cell lines.

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