Blood plasma concentrations of microelements in endurance trained volunteers during hypokinesia and chronic hyperhydration

1994 ◽  
Vol 41 (3) ◽  
pp. 253-267 ◽  
Author(s):  
Yan G. Zorbas ◽  
Youri F. Federenko ◽  
Konstantin A. Naexu
Keyword(s):  
Blood Plasma ◽  
Plasma Concentrations ◽  
Chronic Hyperhydration

scholarly journals Plasma level and expression of visfatin in the porcine hypothalamus during the estrous cycle and early pregnancy

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tadeusz Kaminski ◽  
Marta Kiezun ◽  
Ewa Zaobidna ◽  
Kamil Dobrzyn ◽  
Barbara Wasilewska ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Estrous Cycle ◽  
Early Pregnancy ◽  
Energy Homeostasis ◽  
Plasma Concentrations ◽  
Tissue Samples ◽  
Hormone Synthesis ◽  
Energy Sensor ◽  
Gene And Protein Expression ◽  
Hormonal Milieu

AbstractVisfatin appears to be an energy sensor involved in the regulation of female fertility, which creates a hormonal link integrating the control of energy homeostasis and reproduction. This study evaluates the expression levels of visfatin gene and protein in selected areas of the porcine hypothalamus responsible for gonadotropin-releasing hormone synthesis: the mediobasal hypothalamus (MBH) and preoptic area (POA), and visfatin concentrations in the blood plasma. The tissue samples were harvested from gilts on days 2–3, 10–12, 14–16, and 17–19 of the estrous cycle, and on days 10–11, 12–13, 15–16, 27–28 of pregnancy. Visfatin was localized in the cytoplasm and nucleus of cells creating both studied hypothalamic structures. The study demonstrated that visfatin gene and protein expression in MBH and POA depends on hormonal status related to the phase of the estrous cycle or early pregnancy. Blood plasma concentrations of visfatin during the estrous cycle were higher on days 2–3 in relation to other studied phases of the cycle, while during early pregnancy, the highest visfatin contents were observed on days 12–13. This study demonstrated visfatin expression in the porcine hypothalamus and its dependence on the hormonal milieu related to the estrous cycle and early pregnancy.

scholarly journals Plasma Level and Expression of Visfatin in the Porcine Hypothalamus During the Estrous Cycle and Early Pregnancy

2020 ◽  
Author(s):  
Tadeusz Kaminski ◽  
Marta Kiezun ◽  
Ewa Zaobidna ◽  
Kamil Dobrzyń ◽  
Barbara Wasilewska ◽  
...  
Keyword(s):  
Protein Expression ◽  
Blood Plasma ◽  
Estrous Cycle ◽  
Early Pregnancy ◽  
Energy Homeostasis ◽  
Plasma Concentrations ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tissue Samples ◽  
Hormone Synthesis ◽  
Gene And Protein Expression

Abstract BackgroundVisfatin exists in two forms: the intracellular form which is a rate limiting enzyme engaged in nicotinamide adenine dinucleotide biosynthesis and the extracellular form considered as an adipokine, produced mainly by the adipose tissue. Visfatin could be an energy sensor involved in regulating female fertility, which creates a hormonal link integrating the control of energy homeostasis and reproduction. MethodsThe study compares the expression levels of visfatin gene (quantitative real time PCR) and protein (Western blotting and fluorescent immunohistochemistry) in the selected areas of the porcine hypothalamus responsible for gonadotropin releasing hormone synthesis: the mediobasal hypothalamus (MBH) and preoptic area (POA), and visfatin concentrations in the blood plasma (enzyme-linked immunosorbent assay). The tissue samples were harvested from gilts on days 2-3, 10-12, 14-16 and 17-19 of the estrous cycle, and on days 10-11, 12-13, 15-16, 27-28 of pregnancy. Differences between groups were analyzed by one-way ANOVA followed by Tukey’s post hoc test. ResultsDuring the estrous cycle, visfatin mRNA expression in the MBH was higher on days 2-3 and 17-19, while the visfatin protein concentration on days 17-19. During early pregnancy, the most pronounced gene and protein expression in the MBH was found on days 15-16 and 10-11, respectively. In the POA during the estrous cycle, visfatin gene expression was the highest on days 17-19, and the protein level of visfatin on days 14-16. During early pregnancy, the highest expression of visfatin gene in the POA was observed on days 15-16, whereas the protein concentrations on days 27-28. Blood plasma concentrations of visfatin during the estrous cycle were higher on days 2-3 in relation to other studied phases of the cycle. During early pregnancy, the highest visfatin contents in the blood plasma were observed on days 12-13. Visfatin gene and protein expression in MBH and POA, and visfatin plasma concentrations differed during early pregnancy in relation to days 10-12 of the cycle. ConclusionsThis study demonstrated visfatin expression in the porcine hypothalamus and its dependence on hormonal milieu related to the estrous cycle and early pregnancy.

Blood plasma concentrations of chlortetracycline achieved by administration of a mineral formulation to adult beef cows

2020 ◽  
Vol 36 (1) ◽  
pp. 8-18
Author(s):  
N. Schrag ◽  
K. Hope ◽  
K.C. Olson ◽  
G. Magnin ◽  
Y. Zhang ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Plasma Concentrations ◽  
Beef Cows

Renal regulation of sodium, potassium and chloride balance in single- and twin-pregnant goats

2010 ◽  
Vol 58 (2) ◽  
pp. 199-209 ◽  
Author(s):  
Katarzyna Michałek ◽  
Dorota Jankowiak ◽  
Małgorzata Ożgo ◽  
Wiesław Skrzypczak
Keyword(s):  
Blood Plasma ◽  
Filtration Rate ◽  
Plasma Concentrations ◽  
Chloride Ions ◽  
The Other ◽  
Electrolyte Balance ◽  
Sodium Potassium ◽  
Plasma And Urine Samples ◽  
Potassium Clearance ◽  
Sodium Clearance

The aim of these studies was to analyse and compare changes in selected parameters of renal function in terms of water-electrolyte balance regulation in single- and twin-pregnant goats. Clearance analyses were carried out on 16 pregnant White Improved goats (8 in single and 8 in twin gestation). Blood plasma and urine samples were analysed for the concentration of inulin, endogenous creatinine, sodium, potassium, and chlorides. It has been demonstrated that glomerular filtration rate (GFR) in the goat kidney does not change significantly during gestation. GFR recorded from the 1st week until the 20th week of gestation in twin-pregnant goats was only slightly higher compared to those observed in single-pregnant does. Blood plasma concentrations of major electrolytes, i.e. sodium, potassium and chloride ions, did not differ significantly in pregnant and non-pregnant goats, and remained within the reference values. From the very beginning of gestation, the single-pregnant goats showed increased renal potassium clearance; however, the level of sodium clearance remained stable. On the other hand, sodium clearance increased from the 2nd month of gestation in the twin-pregnant goats, while the load of excreted potassium did not change. These changes had probably resulted from varied levels of aldosterone and progesterone and their mutual proportions differing between the groups.

Blood Plasma Concentrations of Insulin-like Growth Factor-I (IGF-I) in Resting Standardbred Horses

2002 ◽  
Vol 163 (1) ◽  
pp. 45-50 ◽  
Author(s):  
Z.J. Champion ◽  
B.H. Breier ◽  
W.E. Ewen ◽  
T.T. Tobin ◽  
P.J. Casey
Keyword(s):  
Growth Factor ◽  
Blood Plasma ◽  
Plasma Concentrations ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Igf I ◽  
Growth Factor I

Cyclical changes in the uterine endometrium and peripheral plasma concentrations of progesterone in the marsupial Trichosurus vulepcula

1973 ◽  
Vol 21 (1) ◽  
pp. 1 ◽  
Author(s):  
CD Shorey ◽  
RL Hughes
Keyword(s):  
Blood Plasma ◽  
Peripheral Blood ◽  
Plasma Concentrations ◽  
Secretory Activity ◽  
Oestrous Cycle ◽  
Uterine Endometrium ◽  
Pregnant Females ◽  
Peripheral Plasma ◽  
Functional Differences ◽  
Peripheral Blood Plasma

The proliferation and secretory activity of the uterine endometrium in the marsupial T. vulpecula is examined at the cellular and subcellular levels throughout the 26-day oestrous cycle. The observations described are correlated with measured concentrations of progesterone in the peripheral blood plasma. Evidence cited indicates that there are no significant functional differences in the uterine endometrial secretory activity during the 17.5-day gestation period in pregnant females, compared with those in a normal oestrous cycle. Progesterone assays carried out on blood plasma taken from 20 staged animals throughout the oestrous cycle, five of which were at known stages of gestation, also supports the view that pregnancy does not significantly alter the physiological pattern of the reproductive cycle in this marsupial.

Effects of fasting and refeeding on the antioxidant system in cockerels and pullets

2007 ◽  
Vol 55 (2) ◽  
pp. 181-189 ◽  
Author(s):  
Suzana Milinković-Tur ◽  
Z. Stojević ◽  
Jasna Piršljin ◽  
Maja Zdelar-Tuk ◽  
Nina Poljičak-Milas ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Uric Acid ◽  
Blood Plasma ◽  
Whole Blood ◽  
Plasma Concentrations ◽  
Control Level ◽  
Uric Acid Concentration ◽  
Total Lipids ◽  
Antioxidant Defence System ◽  
Fasting And Refeeding

The effect of fasting and refeeding on total antioxidant status (TAS), glutathione peroxidase (GSH-Px) activity and concentration of some non-enzymatic antioxidant compounds was studied in cockerels and pullets. Blood was collected before and after 48-h fasting and 24 h after refeeding. In cockerels, fasting resulted in a significant decrease of TAS and uric acid concentration. After refeeding, the concentration of TAS remained significantly lower as compared to the control level. At the same time, blood plasma level of total lipids increased in comparison to the control and post-fasting values. In pullets, fasting resulted in a significant decrease of whole blood haemolysate GSH-Px activity and blood plasma concentrations of albumin and uric acid. Simultaneously, a significant increase in total lipids and cholesterol was obtained. In pullets, refeeding resulted in a further decrease of TAS to undetectable values, a significant decrease of blood plasma cholesterol, and a significant increase of GSH-Px in the whole blood haemolysate and in blood plasma uric acid content. The results indicate that fasting has a negative impact on the antioxidant defence system of the blood, which leads to a reduced resistance to oxidative stress in both cockerels and pullets. However, pullets seem to be more susceptible to fasting-provoked oxidative stress than cockerels.

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