Recovering cDNA bands from differential display RT-PCR gels using a transparency film mask

1999 ◽  
Vol 11 (2) ◽  
pp. 195-197
Author(s):  
Jaquelline Germano de Oliveira ◽  
Paulo César Peregrino Feneira ◽  
Ema Geessien Kroon
Keyword(s):  
Differential Display ◽  
Rt Pcr ◽  
Transparency Film

scholarly journals Application of the differential display RT-PCR strategy for the identification of inflammation-related mouse genes

1999 ◽  
Vol 32 (7) ◽  
pp. 845-852 ◽  
Author(s):  
A.M. Silva ◽  
E.G. Pires ◽  
E.F. Abrantes ◽  
L.R.P. Ferreira ◽  
R.T. Gazzinelli ◽  
...  
Keyword(s):  
Differential Display ◽  
Rt Pcr

Identification of Genes Induced by Taxol Application Using a Combination of Differential Display RT-PCR and DNA Microarray Analysis

2001 ◽  
Vol 56 (9-10) ◽  
pp. 814-819 ◽  
Author(s):  
Ei-ichiro Fukusaki ◽  
Takashi Oishi ◽  
Hozumi Tanaka ◽  
Shin-ichiro Kajiyama ◽  
Akio Kobayashi
Keyword(s):  
Microarray Analysis ◽  
Dna Microarray ◽  
Differential Display ◽  
Gene Expression Pattern ◽  
Cdna Clones ◽  
Rt Pcr ◽  
Dna Microarray Analysis ◽  
Glass Slides ◽  
Polymerase Chain ◽  
Cdna Fragments

Abstract The differential display reverse transcriptional polymerase chain reaction (DD-RT-PCR) was used to hunt for cDNA fragments specifically expressed by taxol treatment of HeLa cells. Forty-eight cDNA clones were differentially displayed through the experiments. The cDNA fragments obtained were separately spotted onto glass slides to prepare a tailor-made DNA chip. The gene expression pattern of differentially displayed cDNA fragments were checked by DNA microarray analysis.

Differential Gene Expression During Somatic Embryogenesis in Coffea arabica L., Revealed by RT-PCR Differential Display

2002 ◽  
Vol 21 (1) ◽  
pp. 043-050 ◽  
Author(s):  
R Rojas-Herrera ◽  
F Quiroz-Figueroa ◽  
M Monforte-González ◽  
L Sánchez-Teyer ◽  
V M Loyola-Vargas
Keyword(s):  
Gene Expression ◽  
Somatic Embryogenesis ◽  
Differential Gene Expression ◽  
Differential Display ◽  
Coffea Arabica ◽  
Rt Pcr ◽  
Differential Gene ◽  
Coffea Arabica L

180 Inventory of “atherosclerotic genes” induced or repressed in endothelial cells and smooth muscle cells by differential display RT-PCR

1997 ◽  
Vol 11 ◽  
pp. 51
Author(s):  
Anton J.G. Horrevoets ◽  
Carlie J.M. de Vries ◽  
A.E. van Achterberg ◽  
Ruud D. Fontijn ◽  
Benien van Aken ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Differential Display ◽  
Muscle Cells ◽  
Rt Pcr

Molecular cloning of oxygen-inducible genes in Caenorhabditis elegans by RT-PCR differential display

1996 ◽  
Vol 3 (2) ◽  
pp. 107-110 ◽  
Author(s):  
Yoshitaka Kushibiki ◽  
Naoaki Ishii ◽  
Sumino Yanase ◽  
Hiroe Nakazawa
Keyword(s):  
Caenorhabditis Elegans ◽  
Molecular Cloning ◽  
Differential Display ◽  
Rt Pcr ◽  
Inducible Genes

scholarly journals mkp-1 Encoding Mitogen-Activated Protein Kinase Phosphatase 1, a Verotoxin 1 Responsive Gene, Detected by Differential Display Reverse Transcription-PCR in Caco-2 Cells

2000 ◽  
Vol 68 (5) ◽  
pp. 2791-2796 ◽  
Author(s):  
Shihoko Kojima ◽  
Itaru Yanagihara ◽  
Gengo Kono ◽  
Tomomi Sugahara ◽  
Hatsumi Nasu ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Differential Display ◽  
Reverse Transcription ◽  
Mitogen Activated Protein Kinase ◽  
28S Rrna ◽  
Rt Pcr ◽  
Reverse Transcription Pcr ◽  
Mitogen Activated Protein ◽  
A Subunit ◽  
Differential Display Reverse Transcription

ABSTRACT The major cytotoxic effect of the verotoxins (VTs) produced by strains of VT-producing Escherichia coli is the inhibition of host-cell protein synthesis, but VTs are also suspected to play a role in apoptotic cell signaling and cytokine release. Four differentially expressed genes, including mkp-1 (encoding mitogen-activated protein kinase phospatase 1), were detected by differential display reverse transcription-PCR (DD RT-PCR) stimulated by VT1 in Caco-2 cells. Northern blot analysis showed the induction ofmkp-1 mRNA 6 h after VT1 stimulation. Neither mutant VT1 (mutVT1), harboring two mutations in the A subunit (E167Q-R170L), nor cycloheximide induced mkp-1 mRNA, but mkp-1mRNA was detected with both wild-type VT1 (wtVT1) and anisomycin, a 28S rRNA inhibitor. Therefore, we concluded that the A subunit of VT1 was essential for mkp-1 induction. Increased amounts of phosphorylated c-Jun protein were also found with wtVT1 and anisomycin. Although the precise mechanism of induction of MKP-1 is unknown, we hypothesized that 28S rRNA not only was a sensor for ribotoxic stress, but also was involved in the signal cascade of MKP-1. This is the first report of detection by DD RT-PCR of cellular genes induced by bacterial toxins.

scholarly journals Caracterização de ER49, um fator de elongação da síntese de proteínas do tipo Ts, expresso durante a maturação do fruto de tomate

2002 ◽  
Vol 14 (1) ◽  
pp. 21-30 ◽  
Author(s):  
Ana Lúcia S. Chaves ◽  
J.A. Silva ◽  
J.-C. Pech ◽  
A. Latche ◽  
M. Bouzayen ◽  
...  
Keyword(s):  
Lycopersicon Esculentum ◽  
Southern Blot ◽  
Differential Display ◽  
Rt Pcr ◽  
Lycopersicon Esculentum Mill

O etileno, conhecido, sobretudo, como o fitormônio da maturação de frutos, está envolvido em vários processos fisiológicos, atuando na regulação da expressão de grande número de genes. O clone parcial de cDNA ER49 foi previamente isolado mediante a técnica do "Differential Display". A obtenção e a análise da seqüência completa confirmaram as homologias significativas com fatores de elongação da síntese de proteínas do tipo Ts. Tais proteínas são responsáveis pela reciclagem do complexo EF-Tu•GDP, que atua na ligação do aminoacil-RNAt ao ribossomo. A predição da seqüência protéica permitiu a detecção de um peptídio sinal de endereçamento para a mitocôndria e dos domínios TS-N e EF-Ts. A modelagem da estrutura terciária mostrou que ER49 poderia interagir com EF-Tu•GDP, para promover a reciclagem de GDP em GTP. Estudos de expressão durante a maturação do fruto de tomate (Lycopersicon esculentum Mill.), var. Micro-Tom, foram realizados por RT-PCR, revelando que ER49 tem sua expressão induzida nos últimos estádios de maturação do fruto. Os resultados da análise do DNA genômico por Southern blot sugerem que ER49 faz parte de uma família multigênica de 2-3 elementos. Através de ER49, gene mitocondrial regulado pelo etileno, surgem novas perspectivas para o estudo da regulação de genes em nível pós-transcricional e de sua relação com o metabolismo respiratório.

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