The adrenal cortex in ectopic adrenocorticotropic hormone syndrome: A morphological study with histology, transmission and scanning electron microscopy, flow cytometry, and image analysis

1990 ◽  
Vol 1 (3) ◽  
pp. 183-191 ◽  
Author(s):  
Kwok H. Li ◽  
Sylvia L. Asa ◽  
Kalman Kovacs ◽  
David Murray ◽  
William Singer
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Flow Cytometry ◽  
Image Analysis ◽  
Adrenal Cortex ◽  
Morphological Study ◽  
Adrenocorticotropic Hormone ◽  
Ectopic Adrenocorticotropic Hormone ◽  
Scanning Electron

scholarly journals Microtubule organization within mitotic spindles revealed by serial block face scanning electron microscopy and image analysis

2017 ◽  
Vol 130 (10) ◽  
pp. 1845-1855 ◽  
Author(s):  
Faye M. Nixon ◽  
Thomas R. Honnor ◽  
Nicholas I. Clarke ◽  
Georgina P. Starling ◽  
Alison J. Beckett ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Image Analysis ◽  
Mitotic Spindles ◽  
Microtubule Organization ◽  
Face Scanning ◽  
Block Face ◽  
Scanning Electron

Measuring Coral Skeletal Architecture Using Image Analysis v1

2021 ◽  
Author(s):  
Rowan Mclachlan ◽  
Ashruti Patel ◽  
Andrea G Grottoli
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Image Analysis ◽  
Scleractinian Coral ◽  
Coral Species ◽  
Energy Materials ◽  
Simple Method ◽  
Coral Skeletons ◽  
Skeletal Structures ◽  
Scanning Electron

Coral morphology is influenced by genetics, the environment, or the interaction of both, and thus is highly variable. This protocol outlines a non-destructive and relatively simple method for measuring Scleractinian coral sub-corallite skeletal structures (such as the septa length, theca thickness, and corallite diameter, etc.) using digital images produced as a result of digital microscopy or from scanning electron microscopy. This method uses X and Y coordinates of points placed onto photomicrographs to automatically calculate the length and/or diameter of a variety of sub-corallite skeletal structures in the Scleractinian coral Porites lobata. However, this protocol can be easily adapted for other coral species - the only difference may be the specific skeletal structures that are measured (for example, not all coral species have a pronounced columella or pali, or even circular corallites). This protocol is adapted from the methods described in Forsman et al. (2015) & Tisthammer et al. (2018). There are 4 steps to this protocol: 1) Removal of Organic Tissue from Coral Skeletons 2) Imaging of Coral Skeletons 3) Photomicrograph Image Analysis 4) Calculation of Corallite Microstructure Size This protocol was written by Dr. Rowan McLachlan and was reviewed by Ashruti Patel and Dr. Andréa Grottoli. Acknowledgments Leica DMS 1000 and Scanning Electron Microscopy photomicrographs used in this protocol were acquired at the Subsurface Energy Materials Characterization and Analysis Laboratory (SEMCAL), School of Earth Sciences at The Ohio State University, Ohio, USA. I would like to thank Dr. Julie Sheets, Dr. Sue Welch, and Dr. David Cole for training me on the use of these instruments.

scholarly journals Defects of copper patina / Vady mìdìných patin

2015 ◽  
Vol 59 (3) ◽  
pp. 87-90 ◽  
Author(s):  
J. Stoulil ◽  
P. Šedá ◽  
M. Anisová ◽  
Z. Fencl ◽  
P. Novák ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Image Analysis ◽  
Compact Surface ◽  
Historical Buildings ◽  
X Ray Diffraction ◽  
X Ray ◽  
Destructive Analysis ◽  
High Degree ◽  
Scanning Electron

Abstract The paper is focused on analyses of dark copper patina defects that were formed on one sheet under the same conditions. Roofs of ten historical buildings were studied by image analysis and samples of two roofs were subjected to more detailed destructive analysis. These samples were studied by means of scanning electron microscopy with energy dispersive X-ray spectroscopy, X-ray diffraction and infrared spectroscopy. Both types of patina are composed of brochantite. Green patinas consisted of a pure brochantite and they had a fl at and compact surface. Conversely, black patina contained a high degree of impurities (ammonia cations, nitrates, silicates) and the surface was rough. The proportion of dark patina was higher in south and east facing surfaces, where washing by rainfall is more difficult.

scholarly journals Proeryptotic Activity of 4-Hydroxynonenal: A New Potential Physiopathological Role for Lipid Peroxidation Products

Biomolecules ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 770
Author(s):  
Mario Allegra ◽  
Ignazio Restivo ◽  
Alberto Fucarino ◽  
Alessandro Pitruzzella ◽  
Sonya Vasto ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Lipid Peroxidation ◽  
Scanning Electron Microscopy ◽  
Flow Cytometry ◽  
Endothelial Cells ◽  
Caspase 3 ◽  
Morphological Alterations ◽  
Signalling Molecule ◽  
Free Haemoglobin ◽  
Scanning Electron

Background: Eryptosis is a physiological, apoptosis-like death of injured erythrocytes crucial to prevent premature haemolysis and the pathological sequalae generated by cell-free haemoglobin. When dysregulated, the process is associated to several inflammatory-based pathologies. 4-Hydroxy-trans-2-nonenal (HNE) is an endogenous signalling molecule at physiological levels and, at higher concentrations, is involved in the pathogenesis of several inflammatory-based diseases. This work evaluated whether HNE could induce eryptosis in human erythrocytes. Methods: Measurements of phosphatidylserine, cell volume, intracellular oxidants, Ca++, glutathione, ICAM-1, and ceramide were assessed by flow cytometry. Scanning electron microscopy evaluated morphological alterations of erythrocytes. Western blotting assessed caspases. PGE2 was measured by ELISA. Adhesion of erythrocytes on endothelial cells was evaluated by gravity adherence assay. Results: HNE in the concentration range between 10–100 µM induces eryptosis, morphological alterations correlated to caspase-3 activation, and increased Ca++ levels. The process is not mediated by redox-dependent mechanisms; rather, it strongly depends on PGE2 and ceramide. Interestingly, HNE induces significant increase of erythrocytes adhesion to endothelial cells (ECs) that are in turn dysfunctionated as evident by overexpression of ICAM-1. Conclusions: Our results unveil a new physiopathological role for HNE, provide mechanistic details of the HNE-induced eryptosis, and suggest a novel mechanism through which HNE could exert pro-inflammatory effects.

Pore Structure and Microstructure of Foam Concrete

2010 ◽  
Vol 177 ◽  
pp. 530-532 ◽  
Author(s):  
Xin Gang Yu ◽  
Shi Song Luo ◽  
Yan Na Gao ◽  
Hong Fei Wang ◽  
Yue Xiang Li ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Image Analysis ◽  
Light Microscopy ◽  
Pore Structure ◽  
Digital Image ◽  
Digital Image Analysis ◽  
Foam Concrete ◽  
Structure And Microstructure ◽  
Scanning Electron

The pore structure and microstructure of the foam concrete was analyzed by scanning electron microscopy and light microscopy combined with digital image analysis. The results show that: (1) even-distributed fine and close pores resulting in high strength and low permeability; (2) uneven-distributed large size pores and open pores lead to low strength and high permeability; (3) light microscopy combined with digital image analysis is a cheap and convenient tool fitting for the pore structure analysis of the foam concrete; (4) scanning electron microscopy is very appropriate for the pore structure and microstructure analysis of the foam concrete.

Manufacturing and Evaluation of Porous PLA Nano/Micro Fibres

2016 ◽  
Vol 368 ◽  
pp. 146-149
Author(s):  
Eva Macajová
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Image Analysis ◽  
Polymer Solution ◽  
Process Parameters ◽  
Fibre Diameter ◽  
Solution Composition ◽  
Structure And Morphology ◽  
Spinning Process ◽  
Scanning Electron

This study is mainly focused on the study of pore size and shape, fibre diameter and also on the optimization of polymer solution composition and electrospinning parameters with respect to the final structure and morphology of PLA nano/microfibrous layers. The nano/microfibres were produced by electrospinning from the needle. Except the spinning process parameters, the morphology of nanofibrous layers can be also affected by the composition of the polymer solution and by the used solvents. Variations in technological process allows us to design the shape and form of nanofibrous structures upon request. The morphology of nano/microfibres was observed by scanning electron microscopy (SEM). Following image analysis and calculation enables the assessment of porosity contribution to the increase in micro/nanofibre surface area.

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