Agrobacterium-mediated transformation protocol to overcome necrosis in elite AustralianBrassica juncea lines

2006 ◽  
Vol 24 (1) ◽  
pp. 103-103 ◽  
Author(s):  
Anand Pandian ◽  
Clive Hurlstone ◽  
Qing Liu ◽  
Surinder Singh ◽  
Phil Salisbury ◽  
...  
Keyword(s):  
Transformation Protocol ◽  
Agrobacterium Mediated Transformation

scholarly journals Obtaining of transgenic alfalfa (Medicago sativa L.) and peanut (Arachis hypogaea L.) plants resistant to the herbicide Pursuit by Agrobacterium-mediated transformation

1970 ◽  
pp. 243-246
Author(s):  
S. M. Nifantova ◽  
I. K. Komarnytskyi ◽  
M. V. Kuchuk
Keyword(s):  
Gene Selection ◽  
Molecular Size ◽  
Pcr Analysis ◽  
Nptii Gene ◽  
Transformation Protocol ◽  
Genetic Construct ◽  
Agrobacterium Mediated Transformation ◽  
Transgenic Alfalfa ◽  
Arachis Hypogaea L ◽  
Selective Agents

Aim. The production of alfalfa and peanut cultivars with new properties is necessary. The purpose of this work was to develop Agrobacterium-mediated transformation protocol and to construct transgenic alfalfa and peanut plants resistant to herbicide Pursuit Methods. Genetic transformation was carried out using cocultivation of peanut and alfalfa explants with Agrobacterium tumefaciens strain GV3101 carrying genetic construct pCB004 containing mutant ahas/als gene and nptII gene. Selection was held on the solidified callus inducing medium with 50 mkg/l Pursuit. The selected callus clones were put on the regeneration medium with the same selective agents. Obtained regeneration lines were analysed using PCR-analysis. Results. 17 peanut and 14 alfalfa regeneration lines had positive signals after PCR analysis with DNA fragments of required molecular size for ahas/als and nptII genes. Conclusions. Transgenic alfalfa and peanut plants resistant to the herbicide Pursuit were obtained.Keywords: alfalfa, peanut, ahas/als gene, transformation.

An efficient and universal Agrobacterium-mediated transformation protocol in rice

2012 ◽  
Vol 21 (2) ◽  
pp. 252-260 ◽  
Author(s):  
Puspasree Puhan ◽  
Abhilash Vipparla ◽  
Lakshminarayana R. Vemireddy ◽  
G. Anuradha ◽  
E. A. Siddiq
Keyword(s):  
Transformation Protocol ◽  
Agrobacterium Mediated Transformation

A simple and effective method for protein subcellular localization using Agrobacterium-mediated transformation of onion epidermal cells

Biologia ◽  
2007 ◽  
Vol 62 (5) ◽  
Author(s):  
Wei Sun ◽  
Ziyi Cao ◽  
Yan Li ◽  
Yanxiu Zhao ◽  
Hui Zhang
Keyword(s):  
Subcellular Localization ◽  
Particle Bombardment ◽  
Fusion Proteins ◽  
Fluorescent Proteins ◽  
Transformed Cells ◽  
Protein Subcellular Localization ◽  
Transformation Protocol ◽  
Agrobacterium Mediated Transformation ◽  
The Mean ◽  
Onion Epidermal Cells

AbstractA modified Agrobacterium-mediated transformation protocol has been successfully used for transient expression of the intrinsically fluorescent proteins and their fusion proteins in onion epidermis. The mean of the transformed cells rate per peel is about 10.5±0.9%, while that of the particle bombardment method is at the range 2.0±0.4%. To compare with the prevailing method of micro-projectile bombardment, the modified Agrobacterium-mediated transformation may provide with higher efficiency and even more simplified manipulability on a lower budget.

An improved Agrobacterium-mediated transformation protocol for deep water indica rice

2011 ◽  
Author(s):  
Manikandan Ramesh ◽  
Manikandan Ramesh ◽  
Alagarsamy Karthikeyan ◽  
Ramakrishnan Ramesh Kumar ◽  
Shunmugiah Karutha Pandian
Keyword(s):  
Deep Water ◽  
Indica Rice ◽  
Transformation Protocol ◽  
Agrobacterium Mediated Transformation
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