scholarly journals Oxygen consumption and motility of mouse sperm as affected by oxidizable substrates and oxygen tension

1984 ◽  
Vol 49 (2) ◽  
pp. 147-154 ◽  
Author(s):  
E. J. Boell ◽  
J. K. Burkus
Keyword(s):  
Oxygen Consumption ◽  
Oxygen Tension ◽  
Mouse Sperm

Local blood flow, oxygen tension, and oxygen consumption in the rat spinal cord

1983 ◽  
Vol 58 (4) ◽  
pp. 526-530 ◽  
Author(s):  
Nariyuki Hayashi ◽  
Barth A. Green ◽  
Mayra Gonzalez-Carvajal ◽  
Joseph Mora ◽  
Richard P. Veraa
Keyword(s):  
Spinal Cord ◽  
Blood Flow ◽  
Oxygen Consumption ◽  
Oxygen Tension ◽  
Tissue Oxygen ◽  
Local Blood Flow ◽  
Rat Spinal Cord ◽  
Spinal Cord Blood Flow ◽  
Content Type ◽  
Tissue Oxygen Consumption

✓ Using a reliable and reproducible microelectrode technique, consistent simultaneous measurements of local spinal cord blood flow (SCBF), tissue oxygen tension, and tissue oxygen consumption were made at cervical, thoracic, and lumbar levels in the rat spinal cord. These observations showed that the metabolic state is maintained constant along the cord, despite significant variations in vasculature. The physiological and anatomical aspects of these findings are discussed.

Cardiac performance as a function of intracellular oxygen tension in buffer-perfused hearts

2001 ◽  
Vol 281 (6) ◽  
pp. H2463-H2472 ◽  
Author(s):  
Kenneth A. Schenkman
Keyword(s):  
Oxygen Consumption ◽  
Guinea Pig ◽  
Oxygen Tension ◽  
Myocardial Oxygen Consumption ◽  
Arterial Oxygen Tension ◽  
Left Ventricular ◽  
Cardiac Contraction ◽  
Arterial Oxygen ◽  
Adenosine Release ◽  
Lactate Release

Critical intracellular myocardial oxygen tension was determined by optical spectroscopic measurement of myoglobin oxygen saturation in crystalloid-perfused guinea pig hearts. Accurate end-point determinations of the maximally oxygenated and deoxygenated myoglobin were made. Hearts were subjected to a steady decrease in perfusate oxygen tension while left ventricular developed pressure, maximal left ventricular dP/d t, myocardial oxygen consumption, lactate release, and adenosine release were measured as indices of myocardial function. Intracellular myoglobin was found to be only 72% saturated under baseline conditions with an arterial oxygen tension of >600 mmHg at 37°C. Baseline intracellular oxygen tension was 6.3 mmHg. Myocardial oxygen consumption was decreased by 10% when the oxygen tension fell to 5.7 mmHg, and cardiac contraction decreased 10% when oxygen tension was 4.1 mmHg. Adenosine release and, finally, lactate release began to increase at sequentially lower oxygen tensions. The present results indicate that the buffer-perfused guinea pig heart at 37°C has an intracellular oxygen tension just above the threshold for impaired function.

Comparison of buffer and red blood cell perfusion of guinea pig heart oxygenation

2003 ◽  
Vol 285 (5) ◽  
pp. H1819-H1825 ◽  
Author(s):  
Kenneth A. Schenkman ◽  
Daniel A. Beard ◽  
Wayne A. Ciesielski ◽  
Eric O. Feigl
Keyword(s):  
Oxygen Consumption ◽  
Guinea Pig ◽  
Oxygen Saturation ◽  
Red Blood Cells ◽  
Ventricular Function ◽  
Oxygen Tension ◽  
Blood Cells ◽  
Low Oxygen ◽  
Guinea Pig Heart ◽  
Perfused Hearts

Myocardial mean myoglobin oxygen saturation was determined spectroscopically from isolated guinea pig hearts perfused with red blood cells during increasing hypoxia. These experiments were undertaken to compare intracellular myoglobin oxygen saturation in isolated hearts perfused with a modest concentration of red blood cells (5% hematocrit) with intracellular myoglobin saturation previously reported from traditional buffer-perfused hearts. Studies were performed at 37°C with hearts paced at 240 beats/min and a constant perfusion pressure of 80 cmH2O. It was found that during perfusion with a hematocrit of 5%, baseline mean myoglobin saturation was 93% compared with 72% during buffer perfusion. Mean myoglobin saturation, ventricular function, and oxygen consumption remained fairly constant for arterial perfusate oxygen tensions above 100 mmHg and then decreased precipitously below 100 mmHg. In contrast, mean myoglobin saturation, ventricular function, and oxygen consumption began to decrease even at high oxygen tension with buffer perfusion. The present results demonstrate that perfusion with 5% red blood cells in the perfusate increases the baseline mean myoglobin saturation and better preserves cardiac function at low oxygen tension relative to buffer perfusion. These results suggest that caution should be used in extrapolating intracellular oxygen dynamics from buffer-perfused to blood-perfused hearts.

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