Cell-mediated cytotoxicity to rabbit liver-specific lipoprotein in chronic active hepatitis

1982 ◽  
Vol 12 (3) ◽  
pp. 493-499
Author(s):  
Luigi Ortona ◽  
Vittorio Laghi ◽  
Roberto Cauda ◽  
Properzio Nervo ◽  
Maria Ludovica Mausier
Keyword(s):  
Chronic Active Hepatitis ◽  
Rabbit Liver ◽  
Liver Specific Lipoprotein

scholarly journals A species-non-specific liver plasma-membrane antigen and its involvement in chronic active hepatitis

1980 ◽  
Vol 186 (3) ◽  
pp. 679-685 ◽  
Author(s):  
T A De Kretser ◽  
I G McFarlane ◽  
A L Eddleston ◽  
R Williams
Keyword(s):  
Plasma Membrane ◽  
Chronic Active Hepatitis ◽  
Plasma Membranes ◽  
Specific Antigen ◽  
Polymer System ◽  
Two Phase ◽  
Normal Human Liver ◽  
Normal Human ◽  
Electron Microscopical ◽  
Liver Specific Lipoprotein

Rabbit liver plasma membranes were isolated and purified by using an aqueous two-phase polymer system. Examination of these preparations with respect to electron-microscopical appearance, distribution of marker enzymes and gross biochemical composition revealed them to be free from contamination by intracellular components. Sera from ten patients with chronic active hepatitis, four with and six without hepatitis B viral markers (HBsAg) in their sera, produced a single precipitin line on immunodiffusion against a detergent extract of the isolated plasma membranes. Sera from HBsAg-positive and HBsAg-negative patients reacted against the same antigen. This antigen was enriched in the plasma membrane preparations compared with whole-liver homogenates and was identical with a species-non-specific antigen in a macromolecular fraction of normal human liver, which has been previously described as liver-specific lipoprotein.

Qualitative study on “hydropic degeneration” of The hepatocytes in viral hepatitis

1990 ◽  
Vol 48 (3) ◽  
pp. 568-569
Author(s):  
Le Meizhao ◽  
Ye Ming ◽  
Song Xiaoming ◽  
Xu Jiazhang
Keyword(s):  
Qualitative Study ◽  
Viral Hepatitis ◽  
Chronic Active Hepatitis ◽  
Microscopic Observation ◽  
Hydropic Degeneration ◽  
Light Microscopic Observation ◽  
Biopsy Specimens ◽  
Endoplasm Reticulum ◽  
Mixed Fluid ◽  
Peripheral Cytoplasm

“Hydropic degeneration” of the hepatocytes are often found in biopsy of the liver of some kinds of viral hepatitis. Light microscopic observation, compareted with the normal hepatocytes, they are enlarged, sometimes to a marked degree when the term “balloning” degeneration is used. Their cytoplasm rarefied, and show some clearness in the peripheral cytoplasm, so, it causes a hydropic appearance, the cytoplasm around the nuclei is granulated. Up to the present, many studies belive that main ultrastructural chenges of hydropic degeneration of the hepatocytes are results of the RER cristae dilatation with degranulation and disappearance of glycogen granules.The specimens of this study are fixed with the mixed fluid of the osmium acidpotassium of ferricyanide, Epon-812 embed. We have observed 21 cases of biopsy specimens with chronic severe hepatitis and severe chronic active hepatitis, and found that the clear fields in the cytoplasm actually are a accumulating place of massive glycogen. The granules around the nuclei are converging mitochondria, endoplasm reticulum and other organelles.

The Disappearance of Heparin Activity with Liver Globulins and Determinations of Heparinase

1963 ◽  
Vol 10 (01) ◽  
pp. 071-080 ◽  
Author(s):  
L. B Jaques ◽  
C Mary Jaques
Keyword(s):  
Anticoagulant Activity ◽  
Rabbit Liver ◽  
Dissociation Curve ◽  
Ph Values ◽  
Phenol Extraction ◽  
Progressive Loss ◽  
Heparin Activity

SummaryPreparations were made of rabbit liver globulin by the method of Jaques for heparinase and their effect on heparin studied. The results confirmed the observations of a progressive loss of anticoagulant activity with globulin in 0.9% saline, of a loss of metachromatic activity after phenol extraction and the reversal of the latter by alkali. The latter observations were due to the solubility in phenol of heparin on combination with protein. With suitable preparations, a decrease in anticoagulant activity without decrease in metachromatic activity was observed, i.e. conversion of heparin to uroheparin. Loss of heparin due to combination with protein and resulting precipitation, solubility in phenol, etc. followed a protein pH-dissociation curve. Loss of heparin anticoagulant activity due to heparinase was maximal at pH 5.4. No loss of heparin occurred at pH values more acid than 5 or more alkaline than 7.

STOFFWECHSEL VON OESTRON, OESTRADIOL-17α UND OESTRADIOL-17β IN DER KANINCHENLEBER

1960 ◽  
Vol XXXIII (IV) ◽  
pp. 532-538 ◽  
Author(s):  
H. Breuer ◽  
Gerta Pangels
Keyword(s):  
Rabbit Liver ◽  
List Type ◽  
Liver Slices

ABSTRACT The metabolism of oestrone, oestradiol-17α and oestradiol-17β has been studied in rabbit liver slices. Oestradiol-17α and oestradiol-17β were isolated as metabolites of oestrone. When oestradiol-17α17α was incubated oestrone and oestradiol-17β were formed, whereas oestradiol-17β gave rise to oestradiol-17α and oestrone. Quantitative experiments showed that after incubation of oestrone 5–8 times as much oestradiol-17β was found as oestradiol-17α.

Ultrastructural study of the interaction between lymphocytes and hepatocytes in type B chronic active hepatitis patients.

Kanzo ◽  
1986 ◽  
Vol 27 (1) ◽  
pp. 15-19 ◽  
Author(s):  
Osami INOUE ◽  
Hideyo ITAKURA ◽  
Kan TORIYAMA ◽  
Masayoshi SHIMA ◽  
Akira SATO ◽  
...  
Keyword(s):  
Chronic Active Hepatitis ◽  
Ultrastructural Study ◽  
Type B

Characterization of brimonidine metabolism with rat, rabbit, dog, monkey and human liver fractions and rabbit liver aldehyde oxidase

Xenobiotica ◽  
1996 ◽  
Vol 26 (1) ◽  
pp. 1035-1055 ◽  
Author(s):  
A. A. Acheampong ◽  
D-S. Chien ◽  
S. Lam ◽  
S. Vekich ◽  
A. Breau ◽  
...  
Keyword(s):  
Human Liver ◽  
Aldehyde Oxidase ◽  
Rabbit Liver
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