scholarly journals DNA sequence polymorphisms in the genus saccharomyces III. Restriction endonuclease fragment patterns of chromosomal regions in brewing and other yeast strains

1986 ◽  
Vol 51 (3) ◽  
pp. 163-183 ◽  
Author(s):  
Mogens Bohl Pedersen
Keyword(s):  
Restriction Endonuclease ◽  
Dna Sequence ◽  
Yeast Strains ◽  
Sequence Polymorphisms ◽  
Restriction Endonuclease Fragment

Isolation and characterization of plaque-purified strains of Malacosoma disstria Nucleopolyhedrovirus

2006 ◽  
Vol 52 (3) ◽  
pp. 266-271 ◽  
Author(s):  
Martin A Erlandson ◽  
Doug Baldwin ◽  
Melissa Haveroen ◽  
B Andrew Keddie
Keyword(s):  
Restriction Endonuclease ◽  
Cell Line ◽  
Cell Lines ◽  
Field Isolate ◽  
Malacosoma Disstria ◽  
Forest Tent Caterpillar ◽  
Isolation And Characterization ◽  
Budded Virus ◽  
Restriction Endonuclease Fragment ◽  
Tent Caterpillar

Seven plaque-purified genotypic variants or strains, derived from a previously described field isolate of the Malacosoma disstria Nucleopolyhedrovirus (MadiNPV) from Alberta populations of forest tent caterpillar, were characterized based on distinctive restriction endonuclease fragment patterns. Two strains, MadiNPV-pp3 and MadiNPV-pp11, were selected for further characterization, as they represented strains producing high and low budded virus (BV) titres, respectively, in the M. disstria cell line UA-Md203. Analysis of restriction endonuclease fragment profiles indicated the genomes differed significantly in size, 133.8 ± 2.4 kb for MadiNPV-pp3 and 118.1 ± 3.5 kb for MadiNPV-pp11. These strains were characterized based on their BV production in three different cell lines derived from M. disstria haemocytes. Compared with MadiNPV-pp11, MadiNPV-pp3 produced two- to three-fold more BVs in UA-Md203 and 210 other cell lines; however, BV production was only marginally higher for MadiNPV-pp3 in the UA-Md221 cell line. Similarly, the yield of polyhedral inclusion bodies was significantly higher for MadiNPV-pp3 in UA-Md203 and 210 cell lines than for MadiNPV-pp11 but not in the UA-Md221 cell line. This data, although derived from a limited number of cell lines, suggested MadiNPV-pp3 may have a broader tissue tropism than MadiNPV-pp11.Key words: forest tent caterpillar, Malacosoma disstria, Nucleopolyhedrovirus.

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