Immunochemical study of 20 monoclonal antibodies to human plasma low density lipoproteins and their use for apolipoprotein B determination

1984 ◽  
Vol 14 (3) ◽  
pp. 307-312
Author(s):  
Santica Marcovina
Keyword(s):  
Monoclonal Antibodies ◽  
Human Plasma ◽  
Apolipoprotein B ◽  
Low Density Lipoproteins ◽  
Low Density ◽  
Immunochemical Study

Noncompetitive enzyme-linked immunoassay for apolipoprotein B in serum.

1984 ◽  
Vol 30 (1) ◽  
pp. 98-100 ◽  
Author(s):  
C Fievet ◽  
M Koffigan ◽  
D Ouvry ◽  
S Marcovina ◽  
Y Moschetto ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Human Plasma ◽  
Apolipoprotein B ◽  
Solid Phase ◽  
Minimum Detectable Concentration ◽  
Low Density ◽  
Apo B ◽  
Detectable Concentration ◽  
Sensitivity Specificity ◽  
Assay Conditions

Abstract We used a noncompetitive enzyme-linked immunoassay to measure apolipoprotein B (apo-B) concentration in human plasma. Goat anti-lipoprotein B immunoglobulins were adsorbed to the surface of polystyrene balls. After washing, this solid-phase antibody was incubated with antigen (plasma from normal or hyperlipoproteinemic fasting subjects), washed, and then incubated with peroxidase-labeled goat anti-lipoprotein B IgG. After a last washing, we measured the bound label, which provided a direct measurement of the antigen. Under optimized assay conditions, the minimum detectable concentration was 50 ng per assay. The assay may be used to measure apo-B in different lipoprotein fractions (low- or very-low-density) and yields values that compared favorably with those obtained by electroimmunoassay (r = 0.86). The assay offers several advantages over existing techniques: sensitivity, specificity, simplicity, avoidance of radioisotopes, and potential for use with monoclonal antibodies.

Processing of Apolipoprotein B-100 of Human Plasma Low Density Lipoproteins by Tissue and Plasma Kallikreins

1986 ◽  
pp. 195-202 ◽  
Author(s):  
Alan D. Cardin ◽  
Richard L. Jackson ◽  
Virginia H Donaldson ◽  
Julie Chao ◽  
Harry S. Margolius
Keyword(s):  
Human Plasma ◽  
Apolipoprotein B ◽  
Low Density Lipoproteins ◽  
Low Density

Isolation and partial characterization of a polypeptide belonging to apolipoprotein B from low-density lipoproteins of human plasma

Biochemistry ◽  
1980 ◽  
Vol 19 (6) ◽  
pp. 1059-1064 ◽  
Author(s):  
Sven Olof Olofsson ◽  
Kristina Bostroem ◽  
Ulf Svanberg ◽  
Goeran Bondjers
Keyword(s):  
Human Plasma ◽  
Apolipoprotein B ◽  
Low Density Lipoproteins ◽  
Partial Characterization ◽  
Low Density

scholarly journals High-mannose structure of apolipoprotein-B from low-density lipoproteins of human plasma

1985 ◽  
Vol 152 (1) ◽  
pp. 43-50 ◽  
Author(s):  
Matti VAUHKONEN ◽  
Juha VIITALA ◽  
Jaakko PARKKINEN ◽  
Heikki RAUVALA
Keyword(s):  
Human Plasma ◽  
Apolipoprotein B ◽  
Low Density Lipoproteins ◽  
Low Density ◽  
High Mannose

scholarly journals Degradation of apolipoprotein B-100 of human plasma low density lipoproteins by tissue and plasma kallikreins.

1984 ◽  
Vol 259 (13) ◽  
pp. 8522-8528 ◽  
Author(s):  
A D Cardin ◽  
K R Witt ◽  
J Chao ◽  
H S Margolius ◽  
V H Donaldson ◽  
...  
Keyword(s):  
Human Plasma ◽  
Apolipoprotein B ◽  
Low Density Lipoproteins ◽  
Low Density
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