Effect of Nano red elemental selenium on GPx activity of broiler chick kidney cellsin vitro

2003 ◽  
Vol 8 (4) ◽  
pp. 1161-1166 ◽  
Author(s):  
Xu Bao-hua ◽  
Xu Zi-rong ◽  
Xia Mei-sheng ◽  
Hu Cai-hong ◽  
Deng Yue-song ◽  
...  
Keyword(s):  
Elemental Selenium ◽  
Broiler Chick ◽  
Gpx Activity ◽  
Chick Kidney

Fate of Colloidal-Particulate Elemental Selenium in Aquatic Systems

2004 ◽  
Vol 33 (2) ◽  
pp. 559 ◽  
Author(s):  
Yiqiang Zhang ◽  
Zahir A. Zahir ◽  
William T. Frankenberger
Keyword(s):  
Aquatic Systems ◽  
Elemental Selenium

scholarly journals Analysis of Bioavailability and Induction of Glutathione Peroxidase by Dietary Nanoelemental, Organic and Inorganic Selenium

Nutrients ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 1073
Author(s):  
Mitchell T. Ringuet ◽  
Billie Hunne ◽  
Markus Lenz ◽  
David M. Bravo ◽  
John B. Furness
Keyword(s):  
Glutathione Peroxidase ◽  
Similar Efficacy ◽  
Enzymatic Assays ◽  
Germ Free ◽  
Organic Selenium ◽  
Protein Levels ◽  
Antioxidant Protein ◽  
Inorganic Selenium ◽  
Fresh Frozen ◽  
Gpx Activity

Dietary organic selenium (Se) is commonly utilized to increase formation of selenoproteins, including the major antioxidant protein, glutathione peroxidase (GPx). Inorganic Se salts, such as sodium selenite, are also incorporated into selenoproteins, and there is evidence that nanoelemental Se added to the diet may also be effective. We conducted two trials, the first investigated inorganic Se (selenite), organic Se (L-selenomethionine) and nanoelemental Se, in conventional mice. Their bioavailability and effectiveness to increase GPx activity were examined. The second trial focused on determining the mechanism by which dietary Se is incorporated into tissue, utilising both conventional and germ-free (GF) mice. Mice were fed a diet with minimal Se, 0.018 parts per million (ppm), and diets with Se supplementation, to achieve 0.07, 0.15, 0.3 and 1.7 ppm Se, for 5 weeks (first trial). Mass spectrometry, Western blotting and enzymatic assays were used to investigate bioavailability, protein levels and GPx activity in fresh frozen tissue (liver, ileum, plasma, muscle and feces) from the Se fed animals. Inorganic, organic and nanoelemental Se were all effectively incorporated into tissues. The high Se diet (1.7 ppm) resulted in the highest Se levels in all tissues and plasma, independent of the Se source. Interestingly, despite being ~11 to ~25 times less concentrated than the high Se, the lower Se diets (0.07; 0.15) resulted in comparably high Se levels in liver, ileum and plasma for all Se sources. GPx protein levels and enzyme activity were significantly increased by each diet, relative to control. We hypothesised that bacteria may be a vector for the conversion of nanoelemental Se, perhaps in exchange for S in sulphate metabolising bacteria. We therefore investigated Se incorporation from low sulphate diets and in GF mice. All forms of selenium were bioavailable and similarly significantly increased the antioxidant capability of GPx in the intestine and liver of GF mice and mice with sulphate free diets. Se from nanoelemental Se resulted in similar tissue levels to inorganic and organic sources in germ free mice. Thus, endogenous mechanisms, not dependent on bacteria, reduce nanoelemental Se to the metabolite selenide that is then converted to selenophosphate, synthesised to selenocysteine, and incorporated into selenoproteins. In particular, the similar efficacy of nanoelemental Se in comparison to organic Se in both trials is important in the view of the currently limited cheap sources of Se.

scholarly journals Optimization of elemental selenium (Se(0)) determination in yeasts by anion-exchange HPLC-ICP-MS

2021 ◽  
Author(s):  
Véronique Vacchina ◽  
Dominique Foix ◽  
Mathieu Menta ◽  
Hervé Martinez ◽  
Fabienne Séby
Keyword(s):  
Anion Exchange ◽  
Elemental Selenium ◽  
Icp Ms ◽  
Anion Exchange Hplc

scholarly journals Alterations in glutathione redox homeostasis among adolescents with obesity and anemia

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Dalal Alkazemi ◽  
Abdur Rahman ◽  
Banan Habra
Keyword(s):  
Defense Mechanism ◽  
Dynamic Balance ◽  
Redox Homeostasis ◽  
Redox Status ◽  
Cross Sectional Study ◽  
Normal Weight ◽  
Overweight And Obesity ◽  
Metabolic Disturbances ◽  
Cross Sectional ◽  
Gpx Activity

AbstractThe reduced (GSH)-to-oxidized (GSSG) glutathione ratio represents a dynamic balance between oxidants and antioxidants. However, redox status in adolescents with obesity and anemia has not been investigated. This study investigated the association of erythrocyte GSH redox status (GSH, GSH:GSSG ratio, and glutathione peroxidase [GPx] activity) with anemia and adiposity in adolescents. This case–control study nested in a cross-sectional study enrolled 524 adolescents (268 boys; 256 girls). The prevalence of anemia in overweight and obesity (OWOB) was 5.2% in boys and 11.7% in girls. The GSH:GSSG ratio and GPx activity were significantly higher in girls than in boys (p < 0.001), in anemic than in non-anemic subjects (p < 0.001), and in OWOB than in normal-weight subjects (p < 0.001). Similarly, significantly higher GSH: GSSG level (p < 0.001) and GPx activity (p < 0.001) were found in subjects with 90th percentile waist circumference than in those with < 90th percentile. GPx and GSH:GSSG were positively associated with anemia after adjusting for age, sex, and body mass index (adjusted odds ratio, adjOR [95% confidence interval, CI] 2.18 [1.44–3.29]) or tertiles (adjOR [95% CI], T3 = 2.49 [1.03–6.01]). A similar association was noted for GSH and GPx. A compensatory increased redox defense mechanism exists in anemia and obesity among adolescents without metabolic disturbances.

Inducible nitric oxide synthase augments injury elicited by oxidative stress in rat cardiac myocytes

1998 ◽  
Vol 274 (1) ◽  
pp. C245-C252 ◽  
Author(s):  
Junsuke Igarashi ◽  
Masashi Nishida ◽  
Shiro Hoshida ◽  
Nobushige Yamashita ◽  
Hiroaki Kosaka ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Cardiac Myocytes ◽  
Myocardial Injury ◽  
No Production ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
No Donor ◽  
Oxide Synthase ◽  
Gpx Activity

The effects of nitric oxide (NO) produced by cardiac inducible NO synthase (iNOS) on myocardial injury after oxidative stress were examined. Interleukin-1β induced cultured rat neonatal cardiac myocytes to express iNOS. After induction of iNOS,l-arginine enhanced NO production in a concentration-dependent manner. Glutathione peroxidase (GPX) activity in myocytes was attenuated by elevated iNOS activity and by an NO donor, S-nitroso- N-acetyl-penicillamine (SNAP). Although NO production by iNOS did not induce myocardial injury, NO augmented release of lactate dehydrogenase from myocyte cultures after addition of H2O2(0.1 mM, 1 h). Inhibition of iNOS with Nω-nitro-l-arginine methyl ester ameliorated the effects of NO-enhancing treatments on myocardial injury and GPX activity. SNAP augmented the myocardial injury induced by H2O2. Inhibition of GPX activity with antisense oligodeoxyribonucleotide for GPX mRNA increased myocardial injury by H2O2. Results suggest that the induction of cardiac iNOS promotes myocardial injury due to oxidative stress via inactivation of the intrinsic antioxidant enzyme, GPX.

Studies on the nutritive value of full-fat sunflower seed in broiler chick diets

1998 ◽  
Vol 71 (3-4) ◽  
pp. 341-349 ◽  
Author(s):  
M.L Rodrı́guez ◽  
L.T Ortiz ◽  
J Treviño ◽  
A Rebolé ◽  
C Alzueta ◽  
...  
Keyword(s):  
Sunflower Seed ◽  
Nutritive Value ◽  
Broiler Chick

scholarly journals Broiler Chick Thrombocyte Response to Lipopolysaccharide

2008 ◽  
Vol 87 (1) ◽  
pp. 61-63 ◽  
Author(s):  
F. Ferdous ◽  
D. Maurice ◽  
T. Scott
Keyword(s):  
Broiler Chick

Comparative effects of nano elemental selenium and sodium selenite on selenium retention in broiler chickens

2012 ◽  
Vol 177 (3-4) ◽  
pp. 204-210 ◽  
Author(s):  
C.H. Hu ◽  
Y.L. Li ◽  
L. Xiong ◽  
H.M. Zhang ◽  
J. Song ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Sodium Selenite ◽  
Elemental Selenium

Novel Anti-Cancer, Anti-Bacterial Coatings for Biomaterial Applications: Selenium Nanoclusters

2009 ◽  
Vol 1209 ◽  
Author(s):  
Phong Anh Tran ◽  
Erik Taylor ◽  
Love Sarin ◽  
Robert H. Hurt ◽  
Thomas J Webster
Keyword(s):  
Bone Cells ◽  
Tumor Resection ◽  
Active Agent ◽  
In Vitro Study ◽  
Biologically Active ◽  
Elemental Selenium ◽  
Orthopedic Implant ◽  
Functional Coating ◽  
Anti Cancer

AbstractTwo common problems with implantation after cancerous tumor resection are cancer recurrence and bacteria infection at the implant site. Tumor resection surgery sometimes can not remove all the cancerous cells, thus, cancer can return after implantation. In addition, bacteria infection is one of the leading causes of implant failure. Therefore, it is desirable to have anti-cancer and anti-bacterial molecules which both rapidly (for anti-infection purposes) and continuously (for anti-cancer purposes) are available at the implant site following implantation. Therefore, the objective of the present in vitro study was to create a multi-functional coating for anti-cancer and anti-bacterial orthopedic implant applications. Elemental selenium was chosen as the biologically active agent in this effort because of its known chemopreventive and anti-bacterial properties. To achieve that objective, titanium (Ti), a conventional orthopedic implant material was coated with selenium (Se) nanoclusters. Different coating densities were achieved by varying Se concentration in the reaction mixture. Titanium substrates coated with Se nanoclusters were shown to enhance healthy osteoblast (bone-forming cell) and inhibit cancerous osteoblast proliferation in co-culture experiments. Functions of S. epidermidis (one of the leading bacteria that infect implants) were inhibited on Ti coated with Se-nanoclusters compared to uncoated materials. Thus, this study provided for the first time a coating material (selenium nanoclusters) to the biomaterials’ community to promote healthy bone cells’ functions, inhibit cancer growth and prevent bacteria infection.

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