Relationships between nitrate level, nitrate reductase activity and anaerobic nitrite production inPisum sativum leaf tissue

1979 ◽  
Vol 21 (4) ◽  
pp. 307-310 ◽  
Author(s):  
V. Škrdleta ◽  
Alena Gaudinová ◽  
Marie Němcová
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Leaf Tissue ◽  
Nitrate Level ◽  
Nitrite Production

Nitrate Reductase Profiles in Leaves of Tall Morningglory Seedlings

Weed Science ◽  
1973 ◽  
Vol 21 (6) ◽  
pp. 561-564 ◽  
Author(s):  
Donald S. Galitz
Keyword(s):  
Enzyme Activity ◽  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Leaf Growth ◽  
Leaf Tissue ◽  
Plant Leaf ◽  
Ipomoea Purpurea ◽  
True Leaf

Nitrate reductase activity of seed leaves and the first three true leaves of tall morningglory [Ipomoea purpurea (L.) Roth] was shown to increase rapidly initially then gradually decrease. The greatest level of reductase activity of each true leaf was reached at the fourth to fifth day after unfolding. A subsequent leveling off of enzyme activity corresponded to the acceleration of leaf growth. Leaf nitrates were correlated with enzyme activity. The sum of leaf activities was used to estimate the total reductive capacity of the plant leaf tissue at each sampling date.

Sulfur and molybdenum nutrition effects in tall fescue

1981 ◽  
Vol 59 (4) ◽  
pp. 536-541 ◽  
Author(s):  
L. P. Bush ◽  
J. E. Legget ◽  
M. J. King ◽  
J. E. Vincent
Keyword(s):  
Nitrate Reductase ◽  
Tall Fescue ◽  
Festuca Arundinacea ◽  
Culture System ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Leaf Tissue ◽  
Absorption Process ◽  
Small Reduction ◽  
Uptake Studies

Tall fescue (Festuca arundinacea Schreb.) seedlings were grown in an intermittently irrigated gravel culture system in the greenhouse. Nutrient molybdate levels were 0, 0.1, or 0.21 μequiv./L with sulfate levels of 0 to 1.0 mequiv./L. Leaf tissue was harvested 28, 42, and 49 days after seeding. Molybdenum content of leaf tissue increased with increased solution Mo concentration. Increased solution SO42− levels increased tissue S and nitrate reductase activity (NRA), but decreased tissue Mo and NO3− content. When low tissue S was limiting NRA, and when Mo and NO3− were available, NRA was not increased by 2- to 4-h incubations of leaf tissue in SO42− solution. The MoO42− × SO42− interaction for tissue Mo concentration was significant and the effect of SO42− was relatively greater in the presence of Mo than in the absence of Mo.99Mo root uptake studies indicated the first increment of SO42− added to nutrient solution results in the greatest reduction in Mo accumulation. The data indicated that the greatest effect of SO42− on leaf Mo levels occurred during the absorption process with small reduction of Mo translocated from root to leaf tissue.

Reduction of Nitrate Ions by Morningglory Leaves

Weed Science ◽  
1974 ◽  
Vol 22 (1) ◽  
pp. 23-27 ◽  
Author(s):  
Donald S. Galitz
Keyword(s):  
Nitrate Reductase ◽  
Nutrient Medium ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Leaf Tissue ◽  
Maximum Activity ◽  
Decay Rates ◽  
Enzyme Preparations ◽  
Nitrate Ions ◽  
Assay Time

The reduction and assimilation of nitrate ions by 14-day old tall morningglory [Ipomoea purpurea(L.) Roth] seedlings were studied by monitoring the leaf nitrate, protein, and activity of the enzyme nitrate reductase (NADH: nitrate oxidoreductase, EC 1.6.6.1). The pH's of the grinding and assay mixtures yielding maximum nitrate reductase activity were determined to be 8.8 and 7.5 to 7.7, respectively. The maximum assay time for a linear reaction rate for nitrate reductase was estimated at 10 minutes. Decay rates of the enzyme preparations indicated a half-life of 30 min. Requirements for a cofactor to support the reaction were met with the addition of NADH (1 × 10−3M final concentration) to the assay mixture. NADPH was not effective in supporting nitrate reductase activity. Inclusion of cysteine (1 × 10−2M) in the grinding medium was necessary to insure maximum activity of the enzyme preparations. Both light and nitrate were required for nitrate reductase activity in morningglory leaves. Nitrate accumulated in leaf tissue in proportion to the concentration of nitrate in the nutrient medium. Above 7,530 lux luminescence, enzyme activity and tissue protein were related to the light intensity and the availability of nitrate in the nutrient medium on which the plants were grown.

Nitrate Reductase Activity of Maize Hybrids and Their Parental Inbred 1

Crop Science ◽  
1966 ◽  
Vol 6 (2) ◽  
pp. 169-173 ◽  
Author(s):  
L. E. Schrader ◽  
D. M. Peterson ◽  
E. R. Leng ◽  
R. H. Hageman
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Maize Hybrids ◽  
Parental Inbred

Chromosomal Control of Nitrate Reductase Activity in Hexaploid Wheat 1

Crop Science ◽  
1982 ◽  
Vol 22 (1) ◽  
pp. 85-88 ◽  
Author(s):  
E. L. Deckard ◽  
N. D. Williams ◽  
J. J. Hammond ◽  
L. R. Joppa
Keyword(s):  
Nitrate Reductase ◽  
Hexaploid Wheat ◽  
Nitrate Reductase Activity ◽  
Reductase Activity
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