Cytophotometric analysis of glycogen, protein and DNA of a glycogen-storing rat hepatoma (N13) cell line

1991 ◽  
Vol 60 (1) ◽  
pp. 271-278 ◽  
Author(s):  
Robert C. Callaghan ◽  
Rosario Gil-Benso ◽  
Antonio Pellin ◽  
Antonio Llombart-Bosch
Keyword(s):  
Cell Line ◽  
Cytophotometric Analysis ◽  
Rat Hepatoma

scholarly journals Rat hepatoma L35 cells, a liver-differentiated cell line, display resistance to bile acid repression of cholesterol 7 alpha-hydroxylase

1996 ◽  
Vol 37 (3) ◽  
pp. 588-598
Author(s):  
J D Trawick ◽  
K D Lewis ◽  
S Dueland ◽  
G L Moore ◽  
F R Simon ◽  
...  
Keyword(s):  
Bile Acid ◽  
Cell Line ◽  
Rat Hepatoma

Activation of the silent endogenous cholesterol-7-alpha-hydroxylase gene in rat hepatoma cells: a new complementation group having resistance to 25-hydroxycholesterol

1991 ◽  
Vol 11 (4) ◽  
pp. 2049-2056
Author(s):  
J K Leighton ◽  
S Dueland ◽  
M S Straka ◽  
J Trawick ◽  
R A Davis
Keyword(s):  
Cell Line ◽  
Cholesterol Biosynthesis ◽  
Hepatoma Cell ◽  
Regulatory Elements ◽  
Hepatoma Cells ◽  
Complementation Group ◽  
Hepatoma Cell Line ◽  
Cytotoxic Action ◽  
Hydroxylase Activity ◽  
Rat Hepatoma

The oxysterol 25-hydroxycholesterol acts both as a regulatory sterol determining the expression of genes governed by sterol regulatory elements and as a substrate for 7-alpha-hydroxylase, the first and rate-limiting enzyme in the bile acid synthetic pathway. Most wild-type nonhepatic cells are killed by the cytotoxic action of 25-hydroxycholesterol. In contrast, liver cells, which express 7-alpha-hydroxylase activity, are resistant to killing by 25-hydroxycholesterol. We examined the possibility that selection for resistance to 25-hydroxycholesterol might lead to the derivation of a cell line expressing 7-alpha-hydroxylase. A rat hepatoma cell line (7-alpha-hydroxylase minus) was transfected with human DNA and screened for resistance to 25-hydroxycholesterol. Although parental hepatoma cells were all killed within a week, a 25-hydroxycholesterol-resistant cell line (L35 cells) which showed stable expression of 7-alpha-hydroxylase activity and mRNA was obtained. These cells exhibited normal inhibition of cholesterol biosynthesis by 25-hydroxycholesterol. Blocking 7-alpha-hydroxylase activity with ketoconazole also blocked the resistance of L35 cells to 25-hydroxycholesterol. Isolation of microsomes from these cells showed levels of 7-alpha-hydroxylase activity (22.9 pmol/min/mg of protein) that were comparable to the activity (33.2 pmol/min/mg) of microsomes isolated from the livers of rats killed during the high point of the diurnal cycle. Parental cells had no detectable activity. These data show a new complementation group for 25-hydroxycholesterol resistance: expression of 7-alpha-hydroxylase. Dexamethasone increased both the activity and the cellular content of mRNA coding for 7-alpha-hydroxylase. Since dactinomycin blocked the ability of dexamethasone to induce mRNA, active transcription is required. Southern analysis of genomic DNA showed that L35 cells contain the rat (endogenous) gene but not the human gene. Furthermore, the RNA expressed by L35 cells is similar in size to rat RNA and is distinct from the human form of 7-alpha-hydroxylase. The combined data indicate that L35 cells are resistant to 25-hydroxycholesterol because they express 7-alpha-hydroxylase. The mechanism responsible involves activation of the endogenous (silent) gene of the parental rat hepatoma cell.

Specific modulation by ethanol of the protein synthesis pattern in the C2 rat hepatoma cell line

1988 ◽  
Vol 6 (1) ◽  
pp. 85-93 ◽  
Author(s):  
M.N. Chobert ◽  
P. Vincens ◽  
G. Guellaën ◽  
R. Barouki ◽  
Y. Laperche ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Cell Line ◽  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
Rat Hepatoma Cell Line ◽  
Rat Hepatoma

scholarly journals Identification of a somatomedin-binding protein produced by a rat hepatoma cell line

FEBS Letters ◽  
1981 ◽  
Vol 127 (2) ◽  
pp. 225-227 ◽  
Author(s):  
Dan H. Morris ◽  
Don S. Schalch ◽  
Barbara Monty-Miles
Keyword(s):  
Cell Line ◽  
Binding Protein ◽  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
Rat Hepatoma Cell Line ◽  
Rat Hepatoma

scholarly journals Enhanced Na+-dependent bile salt uptake by WIF-B cells, a rat hepatoma hybrid cell line, following growth in the presence of a physiological bile salt

Hepatology ◽  
1998 ◽  
Vol 27 (1) ◽  
pp. 191-199 ◽  
Author(s):  
Elisa M. Konieczko ◽  
Amy K. Ralston ◽  
Aleta R. Crawford ◽  
Saul J. Karpen ◽  
James M. Crawford
Keyword(s):  
B Cells ◽  
Bile Salt ◽  
Cell Line ◽  
Hybrid Cell ◽  
Hybrid Cell Line ◽  
Salt Uptake ◽  
Line Following ◽  
Rat Hepatoma
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