α-Smooth muscle actin is expressed in a subset of bone marrow stromal cells in normal and pathological conditions

1989 ◽  
Vol 57 (1) ◽  
pp. 291-302 ◽  
Author(s):  
Annette Schmitt-Gräff ◽  
Omar Skalli ◽  
Giulio Gabbiani
Keyword(s):  
Bone Marrow ◽  
Smooth Muscle ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Smooth Muscle Actin ◽  
Marrow Stromal Cells ◽  
Muscle Actin ◽  
Pathological Conditions

TGFβ-1 and epithelial-mesenchymal interactions promote smooth muscle gene expression in bone marrow stromal cells: Possible application in therapies for urological defects

2008 ◽  
Vol 31 (11) ◽  
pp. 951-959 ◽  
Author(s):  
C. Becker ◽  
T. Laeufer ◽  
J. Arikkat ◽  
G. Jakse
Keyword(s):  
Bone Marrow ◽  
Smooth Muscle ◽  
Growth Factor ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Smooth Muscle Actin ◽  
Muscle Actin ◽  
Muscle Gene Expression ◽  
Muscle Gene

Purpose For regenerative and cellular therapies of the urinary tract system, autologous bladder smooth muscle cells (SMCs) have several limitations, including constricted in vitro proliferation capacity and, more importantly, inability to be used in malignant conditions. The use of in vitro (pre-)differentiated multipotential adult progenitor cells may help to overcome the shortcomings associated with primary cells. Methods By mimicking environmental conditions of the bladder wall, we investigated in vitro effects of growth factor applications and epithelial-mesenchymal interactions on smooth muscle gene expression and on the morphological appearance of adherent bone marrow stromal cells (BMSCs). Results Transcription growth factor beta-1 (TGFβ-1) upregulated the transcription of myogenic gene desmin and smooth muscle actin-γ2 in cultured BMSCs. Stimulatory effects were significantly increased by coculture with urothelial cells. Prolonged stimulation times and epigenetic modifications further enhanced transcription levels, indicating a dose-response relationship. Immunocytochemical staining of in vitro-differentiated BMSCs revealed expression of myogenic protein α-smooth muscle actin and desmin, and changes in morphological appearance from a fusiform convex shape to a laminar flattened shape with filamentous inclusions similar to the appearance of bladder SMCs. In contrast to the TGFβ-1 action, application of vascular endothelial growth factor (VEGF) did not affect the cells. Conclusions The combined application of TGFβ-1 and epithelial-mesenchymal interactions promoted in vitro outgrowth of cells with a smooth muscle-like phenotype from a selected adherent murine bone marrow-derived cell population.

176: Soluble Substances from Bladder Smooth Muscle Cells Induce Smooth Muscle Cell-Like Phenotype in Bone Marrow Stromal Cells

2004 ◽  
Vol 171 (4S) ◽  
pp. 47-47
Author(s):  
Akihiro Kanematsu ◽  
Shingo Yamamoto ◽  
Eri Iwai-Kanai ◽  
Isao Kanatani ◽  
Yasuhiko Tabata ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Muscle Cells ◽  
Marrow Stromal Cells ◽  
Bladder Smooth Muscle

Smooth muscle-like tissues engineered with bone marrow stromal cells

Biomaterials ◽  
2004 ◽  
Vol 25 (15) ◽  
pp. 2979-2986 ◽  
Author(s):  
Seung-Woo Cho ◽  
Il-Kwon Kim ◽  
Sang Hyun Lim ◽  
Dong-Ik Kim ◽  
Sun-Woong Kang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Smooth Muscle ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells

scholarly journals Serotonin augments smooth muscle differentiation of bone marrow stromal cells

2014 ◽  
Vol 12 (3) ◽  
pp. 599-609 ◽  
Author(s):  
Nobuaki Hirota ◽  
Sarah McCuaig ◽  
Michael J. O'Sullivan ◽  
James G. Martin
Keyword(s):  
Bone Marrow ◽  
Smooth Muscle ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Muscle Differentiation ◽  
Marrow Stromal Cells ◽  
Smooth Muscle Differentiation

Glucocorticoid exposure reduces expression of sclerostin in bone marrow stromal cells

2013 ◽  
Author(s):  
Sylvia Thiele ◽  
Alexander Rauch ◽  
Jan P Tuckermann ◽  
Lorenz C Hofbauer ◽  
Martina Rauner
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells
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