Effect of UTRs from TMV-RNA on the expression of foreign gene in transgenic plants

2000 ◽  
Vol 45 (18) ◽  
pp. 1681-1685
Author(s):  
Hongmin Qin ◽  
Hongnian Guo ◽  
Yantao Jia ◽  
Lihong Li ◽  
Yingchuan Tian
Keyword(s):  
Transgenic Plants ◽  
Foreign Gene

scholarly journals PLANT TRANSFORMATION: ADVANCES AND PERSPECTIVES

1999 ◽  
Vol 56 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Adriana Cristina Alves ◽  
Vera Maria Quecini ◽  
Maria Lucia Carneiro Vieira
Keyword(s):  
Transgenic Plants ◽  
Field Tests ◽  
Basic Research ◽  
Foreign Gene ◽  
In Planta ◽  
Practical Applications ◽  
Biological Remediation ◽  
Tissue Electroporation ◽  
And Performance ◽  
Biochemical Research

Genetic transformation is a powerful tool for plant breeding and genetical, physiological or biochemical research, consequently it is an extremely dynamic field. Transgenic plants are commonly used to complete or substitute mutants in basic research, helping the studies of complex biological situations such as pathogenesis process, genome organization, light reception and signal transduction. In this review, recent approaches for foreign gene introduction (e.g. Agrobiolistics, whole tissue electroporation, in planta Agrobacterium transformation), screening (reporter gene possibilities and performance) and transformant selection (ipt selective marker) are discussed. Transgene expression and mechanisms underlying (trans)gene inactivation are presented. Practical applications of genetically modified plants, field tests and commercial transgenic crops worldwide and in Brazil are listed, as well as the main traits and species modified. Potential uses of transgenic plants for animal compound production, biological remediation and synthetic polymer assembly are also shown.

scholarly journals A Peroxidase Gene Promoter Induced by Phytopathogens and Methyl Jasmonate in Transgenic Plants

1997 ◽  
Vol 10 (3) ◽  
pp. 326-338 ◽  
Author(s):  
Mark D. Curtis ◽  
Anne L. Rae ◽  
Anca G. Rusu ◽  
Stuart J. Harrison ◽  
John M. Manners
Keyword(s):  
Transgenic Plants ◽  
Methyl Jasmonate ◽  
Fungal Pathogen ◽  
Gus Expression ◽  
Histochemical Staining ◽  
Gus Activity ◽  
Microbial Pathogens ◽  
Foreign Gene ◽  
Coding Region ◽  
Peroxidase Gene

The expression of two closely related peroxidase isogenes, Shpx6a and Shpx6b, of the legume Stylosanthes humilis was studied using isogene-specific reverse transcriptase PCR techniques. Results indicated that transcripts of both genes were rapidly induced following inoculation with the fungal pathogen Colletotrichum gloeosporioides, wounding and treatment with the defense regulator methyl jasmonate (MeJA). In contrast, treatment of leaves of S. humilis with abscisic acid (ABA) and salicylic acid (SA) did not induce transcripts of either isogene. A genomic clone containing the Shpx6b gene was isolated and 594 bp of 5′ sequence upstream of the translation start was fused in frame to the coding region of the uidA reporter gene and introduced into tobacco. Expression from the Shpx6b promoter in transgenic plants was determined by histochemical staining and quantitative assays of β-glucuronidase (GUS). In transgenic tobacco, GUS expression was detected in cotyledons, vascular cells of young leaves, anthers, pollen, and the stigma and style. Wounding of the tobacco plants produced very localized GUS staining. Much more extensive staining for GUS was observed following inoculation of tobacco leaves with conidia of the fungal pathogen Cercospora nicotianae and the inoculation of wound sites with mycelium of the Oomycete pathogen Phytophthora parasitica var. nicotianae. Treatment of mature leaves with methyl jasmonate induced GUS activity while treatment with ABA, SA, and H2O2 had no effect. A similar strong induction of GUS activity was measured in young transgenic seedlings germinated on MeJA while some, but much weaker, induction of GUS activity was observed in seedlings treated with SA. The sequence of the promoter contained motifs homologous to putative cis elements in other plant genes responsive to MeJA. The Shpx6b gene is the first plant peroxidase gene shown to be induced by both microbial pathogens and MeJA and its promoter will be useful for investigations of signaling processes during fungal infection and for the expression of foreign gene products at infection sites.

scholarly journals Endeavours of RuBisCO small subunit promoter as a tool of green tissue specific expression

2012 ◽  
Vol 48 (No. 1) ◽  
pp. 1-9 ◽  
Author(s):  
A. Bakhsh ◽  
T. Husnain
Keyword(s):  
Transgenic Plants ◽  
Genetic Engineering ◽  
Transgene Expression ◽  
Expression Patterns ◽  
Small Subunit ◽  
Foreign Gene ◽  
Specific Expression ◽  
Tissue Specific ◽  
Rubisco Small Subunit ◽  
Green Tissue

Transcriptomics has the potential to rapidly increase our knowledge of spatial and temporal gene expression and contributes to the characterization of new promoters for research and development. The successful application of transgenic technology has been further strengthened by the availability of a broad spectrum of promoters having the ability to regulate the temporal and spatial expression patterns of the transgene. A variety of promoters is necessary at all levels of genetic engineering in plants, from basic research discoveries, to development of economically viable crops and plant commodities, to addressing legitimate concerns raised about the safety and containment of transgenic plants in the environment. Compared with the temporal- or spatial-specific expression of the toxin, constitutive expression of foreign proteins in transgenic plants may cause adverse effects. Constitutive overexpression of transgenes that interfere with normal processes in a plant underscores the need for refinement of transgene expression. The development of tissue-specific promoters to drive transgene expression has helped us to fulfil that need. Therefore, in certain circumstances, it is desirable to use expression-specific promoters which express only the foreign gene in specific plant tissues or organs. This review highlights the uses and benefits reaped by researchers by using a green tissue specific promoter, RuBisCO small subunit promoter, in different crops and systems and thus establishing a broad range of tissue specific promoters. Such plant promoters that are activated precisely when and where they are needed would be ideal for genetic engineering strategies.

T-DNA integration patterns in transgenic plants mediated by Agrobacterium tumefaciens

2011 ◽  
Vol 33 (12) ◽  
pp. 1327-1334 ◽  
Author(s):  
Lin YANG ◽  
Feng-Ling FU ◽  
Wan-Chen LI
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Transgenic Plants ◽  
Dna Integration
Sign in / Sign up

Export Citation Format

Share Document