Two virus-encoded RNA silencing suppressors, P14 ofBeet necrotic yellow vein virus and S6 ofRice black streak dwarf virus

2005 ◽  
Vol 50 (4) ◽  
pp. 305-310 ◽  
Author(s):  
Lingdi Zhang ◽  
Zhaohui Wang ◽  
Xianbing Wang ◽  
Dawei Li ◽  
Chenggui Han ◽  
...  
Keyword(s):  
Rna Silencing ◽  
Yellow Vein ◽  
Dwarf Virus ◽  
Black Streak

scholarly journals Rapid screening of RNA silencing suppressors by using a recombinant virus derived from beet necrotic yellow vein virus

2009 ◽  
Vol 90 (10) ◽  
pp. 2536-2541 ◽  
Author(s):  
H. Guilley ◽  
D. Bortolamiol ◽  
G. Jonard ◽  
S. Bouzoubaa ◽  
V. Ziegler-Graff
Keyword(s):  
Rna Silencing ◽  
Plant Defence ◽  
Plant Viruses ◽  
Yellow Vein ◽  
Rapid Screening ◽  
Silencing Suppressor ◽  
Defence Mechanisms ◽  
Simple Method ◽  
Yellow Dwarf Virus ◽  
Plant Defence Mechanisms

To counteract plant defence mechanisms, plant viruses have evolved to encode RNA silencing suppressor (RSS) proteins. These proteins can be identified by a range of silencing suppressor assays. Here, we describe a simple method using beet necrotic yellow vein virus (BNYVV) that allows a rapid screening of RSS activity. The viral inoculum consisted of BNYVV RNA1, which encodes proteins involved in viral replication, and two BNYVV-derived replicons: rep3–P30, which expresses the movement protein P30 of tobacco mosaic virus, and rep5–X, which allows the expression of a putative RSS (X). This approach has been validated through the use of several known RSSs. Two potential candidates have been tested and we show that, in our system, the P13 protein of burdock mottle virus displays RSS activity while the P0 protein of cereal yellow dwarf virus-RPV does not.

Characterization of an RNA silencing suppressor encoded by maize yellow dwarf virus-RMV2

Virus Genes ◽  
2018 ◽  
Vol 54 (4) ◽  
pp. 570-577 ◽  
Author(s):  
Fang Wang ◽  
Xia Zhao ◽  
Qing Dong ◽  
Benguo Zhou ◽  
Zhengliang Gao
Keyword(s):  
Rna Silencing ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Silencing Suppressor ◽  
Rna Silencing Suppressor ◽  
Yellow Dwarf Virus

scholarly journals Evidence That RNA Silencing-Mediated Resistance to Beet necrotic yellow vein virus Is Less Effective in Roots Than in Leaves

2005 ◽  
Vol 18 (3) ◽  
pp. 194-204 ◽  
Author(s):  
Ida Bagus Andika ◽  
Hideki Kondo ◽  
Tetsuo Tamada
Keyword(s):  
Nicotiana Benthamiana ◽  
Rna Silencing ◽  
Defense Mechanism ◽  
Virus Titer ◽  
Virus Multiplication ◽  
Open Reading Frame ◽  
Yellow Vein ◽  
Polymyxa Betae ◽  
Small Interfering Rnas ◽  
Reading Frame

In plants, RNA silencing is part of a defense mechanism against virus infection but there is little information as to whether RNA silencing-mediated resistance functions similarly in roots and leaves. We have obtained transgenic Nicotiana benthamiana plants encoding the coat protein readthrough domain open reading frame (54 kDa) of Beet necrotic yellow vein virus (BNYVV), which either showed a highly resistant or a recovery phenotype following foliar rub-inoculation with BNYVV. These phenotypes were associated with an RNA silencing mechanism. Roots of the resistant plants that were immune to foliar rub-inoculation with BNYVV could be infected by viruliferous zoospores of the vector fungus Polymyxa betae, although virus multiplication was greatly limited. In addition, virus titer was reduced in symptomless leaves of the plants showing the recovery phenotype, but it was high in roots of the same plants. Compared with leaves of silenced plants, higher levels of transgene mRNAs and lower levels of transgene-derived small interfering RNAs (siRNAs) accumulated in roots. Similarly, in nontransgenic plants inoculated with BNYVV, accumulation level of viral RNA-derived siRNAs in roots was lower than in leaves. These results indicate that the RNA silencing-mediated resistance to BNYVV is less effective in roots than in leaves.

scholarly journals RNA4-encoded p31 of beet necrotic yellow vein virus is involved in efficient vector transmission, symptom severity and silencing suppression in roots

2007 ◽  
Vol 88 (5) ◽  
pp. 1611-1619 ◽  
Author(s):  
Muhammad Danial Rahim ◽  
Ida Bagus Andika ◽  
Chenggui Han ◽  
Hideki Kondo ◽  
Tetsuo Tamada
Keyword(s):  
Sugar Beet ◽  
Rna Silencing ◽  
Viral Rna ◽  
Yellow Vein ◽  
Symptom Expression ◽  
Suppressor Activity ◽  
Vector Transmission ◽  
Efficient Vector ◽  
Silencing Suppression ◽  
Rna Accumulation

RNA3 and RNA4 of beet necrotic yellow vein virus (BNYVV) are not essential for virus multiplication, but are associated with vector-mediated infection and disease development in sugar beet roots. Here, a unique role for RNA4 in virus transmission, virulence and RNA silencing suppression was demonstrated. Mutagenic analysis revealed that the RNA4-encoded p31 open reading frame (ORF) was involved in efficient vector transmission and slight enhancement of symptom expression in some Beta species. No effects of RNA4 on virus accumulation in infected tissue were observed. Furthermore, the p31 ORF was involved in the induction of severe symptoms by BNYVV in Nicotiana benthamiana plants without affecting viral RNA accumulation. In contrast, RNA3-encoded p25, previously identified as a major contributor to symptom induction in sugar beet, had no such effect on N. benthamiana. In two different silencing suppression assays, neither p31 nor p25 was able to suppress RNA silencing in leaves, but the presence of p31 enhanced a silencing suppressor activity in roots without alteration in viral RNA accumulation. Thus, BNYVV p31 plays a multifunctional role in efficient vector transmission, enhanced symptom expression and root-specific silencing suppression.

The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing

2014 ◽  
Vol 180 ◽  
pp. 70-75 ◽  
Author(s):  
Verónica C. Delfosse ◽  
Yamila C. Agrofoglio ◽  
María F. Casse ◽  
Iván Bonacic Kresic ◽  
H. Esteban Hopp ◽  
...  
Keyword(s):  
Rna Silencing ◽  
Dwarf Virus ◽  
P0 Protein ◽  
Systemic Rna ◽  
Cotton Leafroll Dwarf Virus

Replication-associated proteins encoded by Wheat dwarf virus act as RNA silencing suppressors

2014 ◽  
Vol 190 ◽  
pp. 34-39 ◽  
Author(s):  
Yan Liu ◽  
Wen Jin ◽  
Liang Wang ◽  
Xifeng Wang
Keyword(s):  
Rna Silencing ◽  
Dwarf Virus ◽  
Wheat Dwarf Virus ◽  
Associated Proteins

A non-structural protein encoded by Rice Dwarf Virus targets to the nucleus and chloroplast and inhibits local RNA silencing

2020 ◽  
Vol 63 (11) ◽  
pp. 1703-1713
Author(s):  
Feng Gao ◽  
Shanshan Zhao ◽  
Shuzhen Men ◽  
Zhensheng Kang ◽  
Jian Hong ◽  
...  
Keyword(s):  
Rna Silencing ◽  
Dwarf Virus ◽  
Rice Dwarf Virus ◽  
Structural Protein
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