Construction of prokaryotic expression plasmid of mtrC protein ofNeisseria gonorrhoeae and its expression inE. coli

2005 ◽  
Vol 25 (5) ◽  
pp. 582-584 ◽  
Author(s):  
Chen Hongxiang ◽  
Tu Yating ◽  
Lin Nengxing ◽  
Huang Changzheng
Keyword(s):  
Prokaryotic Expression ◽  
Expression Plasmid ◽  
Ofneisseria Gonorrhoeae

scholarly journals Design of a Chimaeric Antigen And Its Use In The Detection of IgG Antibodies Against Rubella Virus

2021 ◽  
Author(s):  
Wenyue Xing ◽  
Li Li ◽  
Jingnan Zhang ◽  
Chunli Ma ◽  
Xin Xue ◽  
...  
Keyword(s):  
Western Blotting ◽  
Rubella Virus ◽  
Prokaryotic Expression ◽  
Practical Significance ◽  
Igg Antibodies ◽  
Serum Samples ◽  
Expression Plasmid ◽  
His Tag ◽  
Specificity And Sensitivity ◽  
Antibody Elisa

Abstract Background: Rubella virus (RV) is the causative agent of rubella or German measles. Although most infections cause only mild self-limited measles-like illness, the infection in pregnant women can cause severe foetal malformation or even miscarriage, especially in the first 3 months of pregnancy. Therefore, it is of great practical significance to establish a simple and sensitive RV detection method.Methods: The partial epitopes of the E1 and E2 proteins from Rubella Virus were selected as the target sites, the sequence of the selected antigenic sites of the E1 and E2 were linked by a linker. The expression plasmid P6T was constructed by inserting the gene into PET-32A + with a His tag. The P6 protein was induced and expressed in Escherichia coli L21 DE3 and purified by nickel column affinity. The protein P6 antigen was identified by Western blotting, and an anti-P6 antibody ELISA was established to test known serum samples to evaluate the capability of this method.Results: After purification, the concentration and purity of the protein P6 were 0.283 mg/mL and more than 80%, respectively. Western blotting showed that the protein P6 could react with rubella virus positive serum. By ELISA, 36 negative sera and 58 positive sera were detected. The coincidence rate, specificity and sensitivity of the ELISA were 88.89%, 84.48% and 84.48%, respectively. The P6 ELISA with a kappa coefficient of 0.709, P<0.05, indicated excellent consistency.Conclusions: The P6 protein with excellent antigenicity obtained from prokaryotic expression followed by chromatography purification could prove useful for early diagnosis of RV infection.

Cloning, Prokaryotic Expression, and Functional Testing of a γ-Gliadin Gene from Wheat Cultivar Shaan 253

2011 ◽  
Vol 37 (1) ◽  
pp. 79-86
Author(s):  
Ming-Xia WANG ◽  
Xiang GAO ◽  
Qi-Jiao CHEN ◽  
Jian DONG ◽  
Wan-Chun ZHAO ◽  
...  
Keyword(s):  
Prokaryotic Expression ◽  
Wheat Cultivar ◽  
Functional Testing

CLONING AND PROKARYOTIC EXPRESSION OF HSP70 cDNA FROM PLANARIAN DUGESIA JAPONICA

2010 ◽  
Vol 36 (2) ◽  
pp. 317-322
Author(s):  
Ke-Xue MA ◽  
Guang-Wen CHEN ◽  
Hao LOU ◽  
Li-Na FEI
Keyword(s):  
Prokaryotic Expression ◽  
Dugesia Japonica

The site-directed mutagenesis and prokaryotic expression of midkine gene in Cynoglossus semilaevis

2013 ◽  
Vol 37 (3) ◽  
pp. 330
Author(s):  
Yanan WANG ◽  
Xudong LIU ◽  
Linlin MU ◽  
Zhipeng LIU ◽  
Chunmei LI ◽  
...  
Keyword(s):  
Prokaryotic Expression ◽  
Cynoglossus Semilaevis ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis
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