A comparative ultrastructural study of the effects of chlorphentermine and triparanol in rat lung and adrenal gland

1972 ◽  
Vol 12 (1) ◽  
pp. 91-103 ◽  
Author(s):  
H. Lüllmann ◽  
R. Lüllmann-Rauch ◽  
G.-H Reil
Keyword(s):  
Adrenal Gland ◽  
Ultrastructural Study ◽  
Rat Lung

scholarly journals Glucocorticoid hormones downregulate histidine decarboxylase mRNA and enzyme activity in rat lung

1998 ◽  
Vol 275 (2) ◽  
pp. L407-L413 ◽  
Author(s):  
Cynthia A. Zahnow ◽  
Pertti Panula ◽  
Atsushi Yamatodani ◽  
David E. Millhorn
Keyword(s):  
Gene Expression ◽  
Enzyme Activity ◽  
Adrenal Gland ◽  
Histidine Decarboxylase ◽  
Mrna Levels ◽  
Glucocorticoid Hormones ◽  
Rat Lung ◽  
Dexamethasone Treatment ◽  
Inflammatory Disorders ◽  
Corticosterone Treatment

Histidine decarboxylase (HDC) is the primary enzyme regulating histamine biosynthesis. Histamine contributes to the pathogenesis of chronic inflammatory disorders such as asthma. Because glucocorticoids are effective in the treatment of asthma, we examined the effects of 6 h of exogenously administered dexamethasone (0.5–3,000 μg/kg ip), corticosterone (0.2–200 mg/kg ip), or endogenously elevated corticosterone (via exposure of rats to 10% oxygen) on HDC expression in the rat lung. HDC transcripts were decreased ∼73% with dexamethasone treatment, 57% with corticosterone treatment, and 50% with exposure to 10% oxygen. Likewise, HDC enzyme activity was decreased 80% by treatment with dexamethasone and corticosterone and 60% by exposure to 10% oxygen. Adrenalectomy prevented the decreases in HDC mRNA and enzyme activity observed in rats exposed to 10% oxygen, suggesting that the adrenal gland is necessary for the mediation of hypoxic effects on HDC gene expression. These results demonstrate that corticosteroids initiate a process that leads to the decrease of HDC mRNA levels and enzyme activity in rat lung.

An ultrastructural study of the macrophages of the carrageenan-induced granuloma in the rat lung

1983 ◽  
Vol 140 (1) ◽  
pp. 29-40 ◽  
Author(s):  
R. R. Bowers ◽  
M. E. Stapleton ◽  
P. D. Lew
Keyword(s):  
Ultrastructural Study ◽  
Rat Lung

scholarly journals Ultrastructural Changes in the Adenohypophysis, Adrenal Gland Activity, and Desynchronization of the Oestrous Cycle following Unpredictable Stress in the Rat

1977 ◽  
Vol 30 (6) ◽  
pp. 559 ◽  
Author(s):  
Irina Pollard ◽  
KD Cairncross
Keyword(s):  
Adrenal Gland ◽  
Ultrastructural Study ◽  
Wistar Rats ◽  
Secretory Activity ◽  
Cell Types ◽  
Ultrastructural Changes ◽  
Cellular Activity ◽  
Stressed Group ◽  
Female Wistar Rats ◽  
Circulating Levels

An ultrastructural study of the adenohypophysis, after exposure of female Wistar rats to a signaled unpredictable 5- and I5-day stress regimen, is described. Cellular activity of the adenohypophysis correlated well with the circulating levels of corticosterone. Intense secretory activity was observed in all tropic cell types at 5 and 15 days although the observed differences generally were greater in the 5-day stressed group. It was observed that the oestrous cycles of 40 and 100 % of the rats became desynchronized over the 5- and I5-day stress period respectively.

Effect of varying noise stress duration on rat adrenal gland: an ultrastructural study

1997 ◽  
Vol 29 (5) ◽  
pp. 597-602 ◽  
Author(s):  
A. Pellegrini ◽  
P. Soldani ◽  
M. Gesi ◽  
P. Lenzi ◽  
G. Natale ◽  
...  
Keyword(s):  
Adrenal Gland ◽  
Ultrastructural Study ◽  
Noise Stress

Ultrastructural Cytopiasmic Changes of Adrenal Cortex During Pregnancy

1969 ◽  
Vol 27 ◽  
pp. 298-299
Author(s):  
T. M. Murad ◽  
Karen Israel ◽  
Jack C. Geer
Keyword(s):  
Adrenal Gland ◽  
Steroid Hormones ◽  
Adrenal Cortex ◽  
Lipid Droplets ◽  
Plasma Cholesterol ◽  
Adrenal Steroids ◽  
Dynamic Changes ◽  
Cortical Cells ◽  
Acetyl Coenzyme A ◽  
Adrenal Cortical Cells

Adrenal steroids are normally synthesized from acetyl coenzyme A via cholesterol. Cholesterol is also shown to enter the adrenal gland and to be localized in the lipid droplets of the adrenal cortical cells. Both pregnenolone and progesterone act as intermediates in the conversion of cholesterol into steroid hormones. During pregnancy an increased level of plasma cholesterol is known to be associated with an increase of the adrenal corticoid and progesterone. The present study is designed to demonstrate whether the adrenal cortical cells show any dynamic changes during pregnancy.

Tumor Diagnosis

1982 ◽  
Vol 40 ◽  
pp. 262-265
Author(s):  
Bruce Mackay
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Differential Diagnosis ◽  
Light Microscopy ◽  
Clinical Examination ◽  
Ultrastructural Study ◽  
Diagnostic Procedures ◽  
Specific Diagnosis ◽  
Transmission Electron ◽  
Microscopic Diagnosis

The broadest application of transmission electron microscopy (EM) in diagnostic medicine is the identification of tumors that cannot be classified by routine light microscopy. EM is useful in the evaluation of approximately 10% of human neoplasms, but the extent of its contribution varies considerably. It may provide a specific diagnosis that can not be reached by other means, but in contrast, the information obtained from ultrastructural study of some 10% of tumors does not significantly add to that available from light microscopy. Most cases fall somewhere between these two extremes: EM may correct a light microscopic diagnosis, or serve to narrow a differential diagnosis by excluding some of the possibilities considered by light microscopy. It is particularly important to correlate the EM findings with data from light microscopy, clinical examination, and other diagnostic procedures.

Further Observations on the Virus of Epizootic Diarrhea of Infant Mice. An Electron Microscopic Study

1967 ◽  
Vol 25 ◽  
pp. 110-111
Author(s):  
W. G. Banfield ◽  
G. Kasnic ◽  
J. H. Blackwell
Keyword(s):  
Electron Microscope ◽  
Infected Cell ◽  
Microscopic Study ◽  
Intestinal Epithelium ◽  
Ultrastructural Study ◽  
Osmium Tetroxide ◽  
Lead Citrate ◽  
Electron Microscopic ◽  
Virus Particles ◽  
Infected Cells

An ultrastructural study of the intestinal epithelium of mice infected with the agent of epizootic diarrhea of infant mice (EDIM virus) was first performed by Adams and Kraft. We have extended their observations and have found developmental forms of the virus and associated structures not reported by them.Three-day-old NLM strain mice were infected with EDIM virus and killed 48 to 168 hours later. Specimens of bowel were fixed in glutaraldehyde, post fixed in osmium tetroxide and embedded in epon. Sections were stained with uranyl magnesium acetate followed by lead citrate and examined in an updated RCA EMU-3F electron microscope.The cells containing virus particles (infected) are at the tips of the villi and occur throughout the intestine from duodenum through colon. All developmental forms of the virus are present from 48 to 168 hours after infection. Figure 1 is of cells without virus particles and figure 2 is of an infected cell. The nucleus and cytoplasm of the infected cells appear clearer than the cells without virus particles.

Glycogen in guinea pig neutrophils: Ultrastructural study of neutrophils at the intradermal site of BCG injection

1983 ◽  
Vol 41 ◽  
pp. 726-727
Author(s):  
Corazon D. Bucana
Keyword(s):  
Bone Marrow ◽  
Guinea Pig ◽  
Electron Density ◽  
Peritoneal Cavity ◽  
Ultrastructural Study ◽  
Guinea Pigs ◽  
Random Distribution ◽  
Glycogen Content ◽  
Polymorphonuclear Neutrophils ◽  
Ultrastructural Studies

In the circulating blood of man and guinea pigs, glycogen occurs primarily in polymorphonuclear neutrophils and platelets. The amount of glycogen in neutrophils increases with time after the cells leave the bone marrow, and the distribution of glycogen in neutrophils changes from an apparently random distribution to large clumps when these cells move out of the circulation to the site of inflammation in the peritoneal cavity. The objective of this study was to further investigate changes in glycogen content and distribution in neutrophils. I chose an intradermal site because it allows study of neutrophils at various stages of extravasation.Initially, osmium ferrocyanide and osmium ferricyanide were used to fix glycogen in the neutrophils for ultrastructural studies. My findings confirmed previous reports that showed that glycogen is well preserved by both these fixatives and that osmium ferricyanide protects glycogen from solubilization by uranyl acetate.I found that osmium ferrocyanide similarly protected glycogen. My studies showed, however, that the electron density of mitochondria and other cytoplasmic organelles was lower in samples fixed with osmium ferrocyanide than in samples fixed with osmium ferricyanide.

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