Mitotic rate and mitotic time in coeliac and non-coeliac duodenal biopsies maintained in organ culture

1981 ◽  
Vol 38 (1) ◽  
pp. 159-167 ◽  
Author(s):  
Gjermund Fluge ◽  
Lage Aksnes
Keyword(s):  
Organ Culture ◽  
Mitotic Rate ◽  
Mitotic Time ◽  
Duodenal Biopsies

scholarly journals The Chronology of the Mitotic Cycle of Human Granulocytopoietic Cells Phase Contrast Studies on Living Cells in Vitro

Blood ◽  
1967 ◽  
Vol 30 (5) ◽  
pp. 557-568 ◽  
Author(s):  
E. G. RONDANELLI ◽  
E. MAGLIULO ◽  
A. GIRALDI ◽  
F. P. CARCÒ
Keyword(s):  
Transit Time ◽  
Phase Contrast ◽  
Mitotic Cycle ◽  
Weighted Average ◽  
Mitotic Rate ◽  
Living Cells ◽  
Time Part ◽  
The Mean ◽  
Mitotic Time

Abstract Data on the mitotic index of human granulocytopoietic cells are presented. From these and from the duration of mitosis directly measured in living cells by phase contrast microscope, the weighted average generation time and the mean compartment transit time are computed. Maturation in granulocytopoietic cells appears to induce a reduction of mitotic indices and mitotic rate and an increase in mitotic time and in mean compartment transit time. Part of the increment in mitotic duration may be due to the acquisition by a part of the granulocytopoietic cells of cytoplasmic peripheral motility or other specialized activities, thus distracting part of the energies destined to mitosis.

scholarly journals EMBRYONIC CHICK INTESTINE IN ORGAN CULTURE

1973 ◽  
Vol 58 (1) ◽  
pp. 64-78 ◽  
Author(s):  
R. A. Corradino
Keyword(s):  
Alkaline Phosphatase ◽  
Organ Culture ◽  
Vitamin D3 ◽  
Calcium Binding ◽  
Large Scale ◽  
Binding Protein ◽  
Mitotic Rate ◽  
Calcium Binding Protein ◽  
Culture Period ◽  
Leucine Incorporation

Duodena from 20-day-old chick embryos can be maintained in large scale organ culture on specially designed stainless-steel grids in contact with serum-free medium for 48 h with excellent preservation of mucosal structure at both the light and electron microscope levels. Although mitotic rate was subnormal, several other factors attest to the essential viability of the cultured intestine: L-leucine incorporation into protein, as well as the synthesis of a specific vitamin D3-induced calcium-binding protein (CaBP), increased over a 48-h culture period, and the electropotential gradient across the intestine was maintained throughout the culture period as was a concentration gradient for calcium. The tissue responded to vitamin D3 in the medium by synthesizing the calcium-binding protein within 6 h and by exhibiting enhanced 45Ca uptake within 12–24 h. Concentrations of vitamin D3, or its 25-hydroxylated derivative, higher than necessary for CaBP induction, also increased the activity of alkaline phosphatase. The 1,25-dihydroxylated derivative of vitamin D3, at a level extremely potent in CaBP induction, did not stimulate alkaline phosphatase. Mucosal to serosal transport of 45Ca could also be measured in everted duodenal sacs, subsequent to culture under similar conditions, and was also increased by vitamin D3 in the medium. Other embryonic organs, esophagus, stomach, liver, pancreas, lung, skin, and muscle, did not produce CaBP in response to vitamin D3 in the culture medium. However, CaBP-synthesizing capacity was present in the entire intestinal tract, exclusive of the rectum. 59Fe and 32P uptake by cultured duodenum were also stimulated by vitamin D3. The system has proven quite useful in the study of the vitamin D-mediated calcium absorptive mechanism but should be applicable to the study of the absorption of other nutrients, drugs, hormones, etc., as well as other studies of intestinal function.

Freeze-etch observations on the tight junctions of sertoli cells grown in organ culture with FSH

1978 ◽  
Vol 36 (2) ◽  
pp. 340-341
Author(s):  
Rita Meyer ◽  
Zoltan Posalaky ◽  
Dennis Mcginley
Keyword(s):  
Organ Culture ◽  
Tight Junctions ◽  
Sertoli Cell ◽  
Germ Cells ◽  
Sertoli Cells ◽  
Barrier Function ◽  
Cell Junction ◽  
Intramembranous Particles ◽  
Junctional Complexes ◽  
Oriented Parallel

The Sertoli cell tight junctional complexes have been shown to be the most important structural counterpart of the physiological blood-testis barrier. In freeze etch replicas they consist of extensive rows of intramembranous particles which are not only oriented parallel to one another, but to the myoid layer as well. Thus the occluding complex has both an internal and an overall orientation. However, this overall orientation to the myoid layer does not seem to be necessary to its barrier function. The 20 day old rat has extensive parallel tight junctions which are not oriented with respect to the myoid layer, and yet they are inpenetrable by lanthanum. The mechanism(s) for the control of Sertoli cell junction development and orientation has not been established, although such factors as the presence or absence of germ cells, and/or hormones, especially FSH have been implicated.

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