Enhancement of human muscle growth in diffusion chambers by bone marrow cells

1982 ◽  
Vol 41 (1) ◽  
pp. 171-180 ◽  
Author(s):  
R. Yarom ◽  
S. Meyer ◽  
O. Carmy ◽  
B. Ghidoni ◽  
R. More
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Muscle Growth ◽  
Human Muscle ◽  
Diffusion Chambers ◽  
Marrow Cells

Osteogenesis in transplants of bone marrow cells

Development ◽  
1966 ◽  
Vol 16 (3) ◽  
pp. 381-390 ◽  
Author(s):  
A. J. Friedenstein ◽  
I. I. Piatetzky-Shapiro ◽  
K. V. Petrakova
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Individual Cell ◽  
Tissue Formation ◽  
Diffusion Chambers ◽  
Subcutaneous Transplantation ◽  
Reticular Cells ◽  
Haemopoietic Cells ◽  
Reticular Tissue ◽  
Marrow Cells

After heterotopic (e.g. subcutaneous) transplantation of bone marrow, haemopoiesis in the graft ceases; reticular tissue develops instead, and later bone is formed (Denis, 1958). The result can be achieved by grafting either free pieces of bone marrow or those placed in diffusion chambers (Petrakova, Tolmacheva & Friedenstein, 1963; Rosin, Freiberg & Sajnek, 1963). In the case of free transplantation the bone formed is later filled with bone marrow. After transplantation in diffusion chambers haemopoiesis does not recur despite the development of a considerable mass of bone in the chambers (Friedenstein, 1965). The population of bone marrow cells is very heterogeneous, including haemopoietic cells, reticular cells and endosteum elements. According to generally accepted views this population is a mixture of individual cell lines capable of mutual transformations within certain limits (Maximov, 1927; Burwell, 1964). After transplantation some of the pathways of differentiation open to bone marrow tissue (formation of reticular and bone tissues) are stimulated, while others (haemopoiesis) are arrested.

scholarly journals Proliferation of Human Bone Marrow Cells in Diffusion Chambers Implanted Into Normal or Irradiated Mice

Blood ◽  
1972 ◽  
Vol 40 (2) ◽  
pp. 163-173 ◽  
Author(s):  
Arne Boyum ◽  
Werner Boecker ◽  
Arland L. Carsten ◽  
Eugene P. Cronkite
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Culture Period ◽  
Diffusion Chambers ◽  
Significant Difference ◽  
Lymphocyte Number ◽  
Marrow Cells

Abstract Diffusion chambers containing normal, human bone marrow cells were implanted in the abdominal cavity of normal and irradiated mice. Granulocytic cells and macrophages proliferated in the chambers. The number of cells in the granulocytic series recovered from the chambers dropped to 60% after 1 day; during the next 7 days it varied between 40% and 60% of the inoculated number of granulocytes, with no difference between irradiated and non-irradiated animals. From day 9 the yield of cells in granulocytic series increased in chambers from irradiated animals, and a higher percentage of cells were in the proliferating pool of the granulocytic series. Simultaneously, the cell yield in chambers from normal animals dropped markedly and consisted mostly of mature granulocytes. In both groups the percentage of eosinophilic cells increased significantly during the last part of the culture period. The enhanced growth in the irradiated mice suggests an increased self-renewal of granulocytic stem cells, leading to a larger yield of differentiated granulocytic cells later in the culture period. A shortened generation time and/or increased cloning efficiency of stem cells may also contribute to the enhanced granulocyte production. The suppression of the immune reactivity by irradiation of the host animals may allow better proliferation by delaying production of cytotoxic antibodies against the xenogenic human cells. The number of macrophages increased gradually, and there was no significant difference between irradiated and nonirradiated animals. The lymphocyte number decreased after implantation and varied between 30% and 50% of the inoculated number. From day 11, the lymphocyte number dropped more in normal animals than in irradiated animals.

scholarly journals The Cultivation of Mouse Bone Marrow in Vivo

Blood ◽  
1959 ◽  
Vol 14 (9) ◽  
pp. 1040-1046 ◽  
Author(s):  
IRWIN BERMAN ◽  
HENRY S. KAPLAN
Keyword(s):  
Bone Marrow ◽  
Peritoneal Cavity ◽  
Normal Mouse ◽  
Bone Marrow Cells ◽  
Mouse Bone Marrow ◽  
Considerable Time ◽  
Diffusion Chambers ◽  
Mouse Bone Marrow Cells ◽  
Marrow Cells

Abstract The cultivation of normal mouse bone marrow cells in diffusion chambers implanted into the peritoneal cavity of mice has been described. Mouse bone marrow cells cultivated by this method continue to undergo differentiation and maintain their morphologic identity for a considerable time.

scholarly journals Kinetics of Cell Proliferation of Murine Bone Marrow Cells Cultured in Diffusion Chambers: Effect of Hypoxia, Bleeding, Erythropoietin Injections, Polycythemia, and Irradiation of the Host

Blood ◽  
1972 ◽  
Vol 40 (2) ◽  
pp. 174-188 ◽  
Author(s):  
Arne Boyum ◽  
Arland L. Carsten ◽  
Ole Didrik Laerum ◽  
Eugene P. Cronkite
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Progenitor Cells ◽  
Bone Marrow Cells ◽  
Abdominal Cavity ◽  
Dilution Method ◽  
Mouse Bone Marrow ◽  
Diffusion Chambers ◽  
Number Of Cells ◽  
Marrow Cells

Abstract Mouse bone marrow cells were cultured in diffusion chambers implanted in the abdominal cavity of host mice that were subjected to different kinds of treatment. Preirradiation of the chamber host enhanced the cell growth in the chambers as follows: the number of cells capable of proliferation and differentiation in the chambers (diffusion chamber progenitor cells, DCPC) as determined by a limiting dilution method increased significantly. Similarly, the spleen colony-forming units (CFU’s) in the chambers proliferated more rapidly in irradiated than in normal hosts. The total number of cells in the granulocytic series harvested after culture periods of 3-7 days was also significantly increased. The number of cells in the granulocytic series harvested per DCPC in irradiated animals was twice normal. These findings indicate that the progenitor cells probably proceed through multiplicative mitoses before differentiation in the irradiated animals. A higher cloning efficiency or a shortened generation time of stem cells are other possibilities. The increased number of differentiating granulocytes may simply be a consequence of the events taking place in the stem cell compartment, but a stimulating effect of the irradiation environment on proliferating cells in the granulocytic series cannot be excluded. Preirradiation also stimulated the growth of macrophages but less consistently. When normal mice carrying chambers with normal marrow were subjected to intermittent hypoxia during the culture period, the number of DCPC’s and of cells in the granulocytic series and macrophages were reduced approximately to the same extent. Hypoxic treatment of donor animals for 3-5 days resulted in a similar reduction in number of DCPC’s and CFU’s at both intervals. These findings may suggest competition for a common pool of stem cells for erythrocytes, granulocytes, and macrophages. However, a toxic effect cannot be excluded. Erythropoiesis, which is usually absent, was significantly increased when hypoxia and irradiation of the chamber hosts were combined. Bleeding and erythropoietin injections also enhanced erythropoiesis to some extent.

Erythropoietic Response of Bone Marrow Cells cultivated in Diffusion Chambers

Nature ◽  
1967 ◽  
Vol 213 (5073) ◽  
pp. 300-301 ◽  
Author(s):  
IRWIN BERMAN ◽  
EARLENE J. NEWBY
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Diffusion Chambers ◽  
Erythropoietic Response ◽  
Marrow Cells
Sign in / Sign up

Export Citation Format

Share Document