Effect of oxygen on the tetrazolium reaction for glucose 6-phosphate dehydrogenase in cryosections of human breast carcinoma, fibrocystic disease and normal breast tissue

1986 ◽  
Vol 50 (1) ◽  
pp. 13-25 ◽  
Author(s):  
Ole William Petersen ◽  
Poul Erik Høyer ◽  
Bo Deurs
Keyword(s):  
Breast Carcinoma ◽  
Breast Tissue ◽  
Normal Breast Tissue ◽  
Normal Breast ◽  
Human Breast Carcinoma ◽  
Human Breast ◽  
Fibrocystic Disease ◽  
Effect Of Oxygen ◽  
Glucose 6 Phosphate Dehydrogenase

DESMOLASE ACTIVITY OF NORMAL AND MALIGNANT HUMAN BREAST TISSUE

1969 ◽  
Vol 44 (1) ◽  
pp. 69-77 ◽  
Author(s):  
J. B. ADAMS ◽  
M. S. F. WONG
Keyword(s):  
Breast Carcinoma ◽  
Breast Tissue ◽  
Normal Breast Tissue ◽  
Radical Mastectomy ◽  
Normal Breast ◽  
Acetone Powder ◽  
Human Breast ◽  
Carcinoma Tissue ◽  
Mastectomy Specimen ◽  
Powder Fraction

SUMMARY [4-14C]Cholesterol was cleaved to pregnenolone and progesterone by a washed particulate fraction isolated from involved lymph nodes obtained from a radical mastectomy specimen. Difficulties were experienced in isolating subcellular fractions from primary breast tumours and normal breast tissue. However, an acetone powder fraction, obtained from normal tissue derived from a radical mastectomy specimen, yielded pregnenolone from [4-14C]cholesterol. [7α-3H]17α-Hydroxyprogesterone was converted to androstenedione and to 17α,20α-dihydroxypregn-4-en-3-one and its 17α,20β-epimer by homogenates of secondary breast carcinoma tissue and normal breast tissue obtained from radical mastectomy specimens. These results, in conjunction with those obtained previously (Adams & Wong, 1968a), show that human breast carcinoma tissue contains the enzymes necessary to convert cholesterol to androgens and oestrogens.

The expression of cytochrome P450 enzymes in human breast tumours and normal breast tissue

2001 ◽  
Vol 70 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Mumtaz Iscan ◽  
Tuula Klaavuniemi ◽  
Tulay Çoban ◽  
Nilgun Kapucuoğlu ◽  
Olavi Pelkonen ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Breast Tissue ◽  
Normal Breast Tissue ◽  
Normal Breast ◽  
Human Breast ◽  
Cytochrome P450 Enzymes ◽  
Breast Tumours

scholarly journals Targeted next-generation sequencing assays using triplet samples of normal breast tissue, primary breast cancer, and recurrent/metastatic lesions

2020 ◽  
Author(s):  
Toshiaki Akahane ◽  
Naoki Kanomata ◽  
Oi Harada ◽  
Tetsumasa Yamashita ◽  
Junichi Kurebayashi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Primary Tumor ◽  
Primary Breast Cancer ◽  
Breast Tissue ◽  
Normal Breast Tissue ◽  
Metastatic Breast ◽  
Normal Breast ◽  
Human Breast ◽  
Metastatic Lesions ◽  
Generation Sequencing

Abstract Background: Next-generation sequencing (NGS) has shown that recurrent/metastatic breast cancer lesions may have additional genetic changes compared with the primary tumor. These additional changes may be related to tumor progression and/or drug resistance. However, breast cancer-targeted NGS is not still widely used in clinical practice to compare the genomic profiles of primary breast cancer and recurrent/metastatic lesions.Methods: Triplet samples of genomic DNA were extracted from each patient’s normal breast tissue, primary breast cancer, and recurrent/metastatic lesion(s). A DNA library was constructed using the QIAseq Human Breast Cancer Panel (93 genes, Qiagen) and then sequenced using MiSeq (Illumina). The Qiagen web portal was utilized for data analysis.Results: Successful results for three or four samples (normal breast tissue, primary tumor, and at least one metastatic/recurrent lesion) were obtained for 11 of 35 breast cancer patients with recurrence/metastases (36 samples). We detected shared somatic mutations in all but one patient, who had a germline mutation in TP53. Additional mutations that were detected in recurrent/metastatic lesions compared with primary tumor were in genes including TP53 (three patients) and one case each of ATR, BLM, CBFB, EP300, ERBB2, MUC16, PBRM1, and PIK3CA. Actionable mutations and/or copy number variations (CNVs) were detected in 73% (8/11) of recurrent/metastatic breast cancer lesions.Conclusions: The QIAseq Human Breast Cancer Panel assay showed that recurrent/metastatic breast cancers sometimes acquired additional mutations and CNV. Such additional genomic changes could provide therapeutic target.

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