Flow microfluorometric analysis of nuclear DNA in cells from solid tumors and cell suspensions

1977 ◽  
Vol 24 (1) ◽  
pp. 227-242 ◽  
Author(s):  
Lars L. Vindeløv
Keyword(s):  
Solid Tumors ◽  
Nuclear Dna ◽  
Cell Suspensions ◽  
In Cells

scholarly journals Geranic Acid Formation, an Initial Reaction of Anaerobic Monoterpene Metabolism in DenitrifyingAlcaligenes defragrans

2000 ◽  
Vol 66 (7) ◽  
pp. 3004-3009 ◽  
Author(s):  
Udo Heyen ◽  
Jens Harder
Keyword(s):  
Culture Medium ◽  
Degradation Pathway ◽  
Unsaturated Hydrocarbon ◽  
Cell Suspensions ◽  
Carbon Limitation ◽  
Initial Reaction ◽  
Activation Reaction ◽  
Geranic Acid ◽  
Monoterpene Metabolism ◽  
In Cells

ABSTRACT Monoterpenes with an unsaturated hydrocarbon structure are mineralized anaerobically by the denitrifying β-proteobacteriumAlcaligenes defragrans. Organic acids occurring in cells ofA. defragrans and culture medium were characterized to identify potential products of the monoterpene activation reaction. Geranic acid (E,E-3,7-dimethyl-2,6-octadienoic acid) accumulated to 0.5 mM in cells grown on α-phellandrene under nitrate limitation. Cell suspensions of A. defragrans65Phen synthesized geranic acid in the presence of β-myrcene, α-phellandrene, limonene, or α-pinene. Myrcene yielded the highest transformation rates. The alicyclic acid was consumed by cell suspensions during carbon limitation. Heat-labile substances present in cytosolic extracts catalyzed the formation of geranic acid from myrcene. These results indicated that a novel monoterpene degradation pathway must be present in A. defragrans.

Abstract 733: Analysis of the hypoxic transcriptome in cells and solid tumors reveals a novel spliced isoform of the key regulator of mRNA translation, eIF2B5

2016 ◽  
Author(s):  
Lauren K. Brady ◽  
Rohil Shekher ◽  
Vladimir Popov ◽  
Mircea Ivan ◽  
Milan Radovich ◽  
...  
Keyword(s):  
Solid Tumors ◽  
Mrna Translation ◽  
In Cells

Erratum to “Molecular mechanism of action of oxazolinoanthracyclines in cells derived from human solid tumors. Part 2” [Toxicol. in Vitro 46 (2018) 323–334]

2018 ◽  
Vol 48 ◽  
pp. 350-352
Author(s):  
Marta Denel-Bobrowska ◽  
Małgorzata Łukawska ◽  
Barbara Bukowska ◽  
Arkadiusz Gajek ◽  
Irena Oszczapowicz ◽  
...  
Keyword(s):  
Solid Tumors ◽  
Molecular Mechanism ◽  
Mechanism Of Action ◽  
Human Solid Tumors ◽  
Molecular Mechanism Of Action ◽  
In Cells

scholarly journals SYNCHRONIZATION OF MITOCHONDRIAL DNA SYNTHESIS IN CHINESE HAMSTER CELLS (LINE CHO) DEPRIVED OF ISOLEUCINE

1973 ◽  
Vol 58 (2) ◽  
pp. 340-345 ◽  
Author(s):  
Kenneth D. Ley ◽  
Marilyn M. Murphy
Keyword(s):  
Cell Cycle ◽  
Mitochondrial Dna ◽  
Dna Synthesis ◽  
Cell Cycle Progression ◽  
Time Course ◽  
Nuclear Dna ◽  
Tritiated Thymidine ◽  
Chinese Hamster ◽  
Chinese Hamster Cells ◽  
In Cells

Mitochondrial DNA (mit-DNA) synthesis was compared in suspension cultures of Chinese hamster cells (line CHO) whose cell cycle events had been synchronized by isoleucine deprivation or mitotic selection. At hourly intervals during cell cycle progression, synchronized cells were exposed to tritiated thymidine ([3H]TdR), homogenized, and nuclei and mitochondria isolated by differential centrifugation. Mit-DNA and nuclear DNA were isolated and incorporation of radioisotope measured as counts per minute ([3H]TdR) per microgram DNA. Mit-DNA synthesis in cells synchronized by mitotic selection began after 4 h and continued for approximately 9 h. This time-course pattern resembled that of nuclear DNA synthesis. In contrast, mit-DNA synthesis in cells synchronized by isoleucine deprivation did not begin until 9–12 h after addition of isoleucine and virtually all [3H]TdR was incorporated during a 3-h interval. We have concluded from these results that mit-DNA synthesis is inhibited in CHO cells which are arrested in G1 because of isoleucine deprivation and that addition of isoleucine stimulates synchronous synthesis of mit-DNA. We believe this method of synchronizing mit-DNA synthesis may be of value in studies of factors which regulate synthesis of mit-DNA.

scholarly journals NUCLEAR FLUORESCENCE EMPLOYING ANTINUCLEOSIDE IMMUNOGLOBULINS

1967 ◽  
Vol 125 (1) ◽  
pp. 61-70 ◽  
Author(s):  
William J. Klein ◽  
Sam M. Beiser ◽  
Bernard F. Erlanger
Keyword(s):  
Dna Synthesis ◽  
Specific Antibody ◽  
Nuclear Dna ◽  
L Cells ◽  
Almost All ◽  
In Cells

Fluoresceinated antinucleoside globulins were shown to react with the nuclei of L cells. The pattern of nuclear fluorescence was similar to the distribution of nuclear DNA. This reaction was shown to be specific by the following control experiments: 1. Absorption of the specific antibody from an antiadenosine globulin eliminated all fluorescence. 2. Treatment of the cells with nonfluorescent antiadenosine globulin, followed by staining with the fluorescent antiadenosine eliminated almost all of the fluorescence of the nucleus. 3. Treatment of the cells with DNase destroyed the ability of the nucleus to react with antiuridine fluorescent antibodies. 4. Fluoresceinated anti-BSA did not produce nuclear fluorescence. Nuclear fluorescence occurred only in cells harvested during the period of maximum DNA synthesis as measured by the uptake of thymidine. This correlates with the previously demonstrated specificity of the antibodies for denatured DNA.

scholarly journals Oxygen-responsive p53 tetramer-octamer switch controls cell fate

2019 ◽  
Author(s):  
Shashank Taxak ◽  
Uttam Pati
Keyword(s):  
Decision Making ◽  
Tumor Suppressor ◽  
Solid Tumors ◽  
Cell Fate ◽  
Control Cell ◽  
Reverse Direction ◽  
Gain Of Function ◽  
Flip Flop ◽  
In Cells

ABSTRACTSolid tumors require an efficient decision-making mechanism to progress through a gradient of hypoxia. Here, we show that an oxygen-sensory p53 tetramer-octamer switch makes cell decision for survival or death in variable hypoxia. Trapping homo-oligomers from biosynthesis cycle, we found a metastable p53 tetramer in cells. Under the operation of switch, tetramer segregates the p53 character of a tumor suppressor and promoter. The p53 switch generates a pattern of its on-off state in time that is specific to the strength of hypoxia. A bidirectional tetramer-octamer conversion in on state decides the restoration of basal state by forward and programs apoptosis upon the reverse shift via p53-MDM2 loop. However, reversible dimertetramer transitions in off state trigger chaperoning of HIF-1 complex by tetramer in forward and oncogenic gain-of-function by prion-like dimers in reverse direction. Temporal on-off patterns calibrate stabilized p53 pool by defining the abundance of dimer, tetramer and octamer that ultimately decides diverse cellular outcomes in hypoxia. Through multi-chromophore FRET, we further show that chaperoning of HIF-1 may modulate angiogenesis through a possible flip-flop of the p53T-HIF-1 complex upon DNA. Our results demonstrate how p53 can sense oxygen and act upon its homo-oligomerization states to control cell fate in hypoxic tumors.

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