Inhibitory effect of cyclosporin A on T lymphocyte DNA synthesis

1992 ◽  
Vol 12 (1) ◽  
pp. 39-42 ◽  
Author(s):  
Li Ming ◽  
Zhang Han ◽  
Yang Jing ◽  
Shao Jing-fang ◽  
Jiang Han-yin
Keyword(s):  
Dna Synthesis ◽  
Cyclosporin A ◽  
T Lymphocyte ◽  
Inhibitory Effect ◽  
Lymphocyte Dna Synthesis

scholarly journals B cell stimulatory factor 1 (interleukin 4) is sufficient for the proliferation and differentiation of lectin-stimulated cytolytic T lymphocyte precursors.

1987 ◽  
Vol 166 (5) ◽  
pp. 1464-1470 ◽  
Author(s):  
J D Pfeifer ◽  
D T McKenzie ◽  
S L Swain ◽  
R W Dutton
Keyword(s):  
T Lymphocyte ◽  
Proliferative Response ◽  
Inhibitory Effect ◽  
Interleukin 4 ◽  
Con A ◽  
Endogenous Production ◽  
Proliferation And Differentiation ◽  
Cytolytic T Lymphocyte ◽  
Stimulatory Factor ◽  
Mitogenic Lectin

In this report, we demonstrate that IL-4 is sufficient to stimulate both the proliferation and differentiation of Lyt-2+, Ia- splenic CTL precursors stimulated with the mitogenic lectin Con A. The response to IL-4 and Con A was not dependent on a putative endogenous production of IL-2 within the cultures, as demonstrated by an absence of an inhibitory effect by an anti-IL-2-R blocking mAb. Our results indicate that IL-2 and IL-4 can support an equivalent proliferative response by lectin-stimulated Lyt-2+ T lymphocytes, while IL-4 is more efficacious in stimulating their differentiation into mature cytolytically active cells.

Role of amidation in bile acid effect on DNA synthesis by regenerating mouse liver

1995 ◽  
Vol 268 (6) ◽  
pp. G1051-G1059
Author(s):  
E. R. Barbero ◽  
M. C. Herrera ◽  
M. J. Monte ◽  
M. A. Serrano ◽  
J. J. Marin
Keyword(s):  
Bile Acid ◽  
Dna Synthesis ◽  
Bile Acids ◽  
High Performance ◽  
Cell Injury ◽  
Intraperitoneal Administration ◽  
Inhibitory Effect ◽  
Toxicity Tests ◽  
Maximal Effect

Effect of bile acids on DNA synthesis by the regenerating liver was investigated in mice in vivo after partial hepatectomy (PH). Radioactivity incorporation into DNA after [14C]thymidine intraperitoneal administration peaked at 48 h after PH. At this time a significant taurocholate-induced dose-dependent reduction in DNA synthesis without changes in total liver radioactivity content was found (half-maximal effect at approximately 0.1 mumol/g body wt). Effect of taurocholate (0.5 mumol/g body wt) was mimicked by chocolate, ursodeoxycholate, deoxycholate, dehydrocholate, tauroursodeoxycholate, taurochenodeoxycholate, and taurodeoxycholate. In contrast, chenodeoxycholate, glycocholate, glycochenodeoxycholate, glycoursodeoxycholate, glycodeoxycholate, 5 beta-cholestane, bromosulfophthalein, and free taurine lacked this effect. No relationship between hydrophobic-hydrophilic balance and inhibitory effect was observed. Analysis by high-performance liquid chromatography indicated that inhibition of thymidine incorporation into DNA was not accompanied by an accumulation of phosphorylated DNA precursors in the liver but rather by a parallel increase in nucleotide catabolism. Bile acid-induced modifications in DNA synthesis were observed in vivo even in the absence of changes in toxicity tests, which suggests that the inhibitory effect shared by most unconjugated and tauroconjugated bile acids but not by glycoconjugated bile acids should be accounted for by mechanisms other than nonselective liver cell injury.

Lymphocyte DNA Synthesis Inhibition

Science ◽  
1971 ◽  
Vol 173 (4002) ◽  
pp. 1139-1141 ◽  
Author(s):  
J. C. Houck ◽  
H. Irausquin ◽  
S. Leikin
Keyword(s):  
Dna Synthesis ◽  
Synthesis Inhibition ◽  
Lymphocyte Dna Synthesis ◽  
Dna Synthesis Inhibition

Effect of cyclosporin-A (CsA) on the ability of T lymphocyte subsets to inhibit the proliferation of autologous EBV-transformed B cells

1985 ◽  
Vol 35 (3) ◽  
pp. 327-333 ◽  
Author(s):  
Maria Teresa Bejarano ◽  
Maria Grazia Masucci ◽  
Ingemar Ernberg ◽  
Eva Klein ◽  
George Klein
Keyword(s):  
B Cells ◽  
Cyclosporin A ◽  
T Lymphocyte ◽  
Lymphocyte Subsets ◽  
T Lymphocyte Subsets

Inhibitory Effect of Ethanol on Hepatocyte Growth Factor-Induced DNA Synthesis and Ca2+ Mobilization in Rat Hepatocytes

1996 ◽  
Vol 20 (s9) ◽  
pp. 330A-334A ◽  
Author(s):  
Katsuhisa Saso ◽  
Katsuyoshi Higashi ◽  
Tomoyuki Nomura ◽  
Makoto Hoshino ◽  
Makoto Ito ◽  
...  
Keyword(s):  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Dna Synthesis ◽  
Rat Hepatocytes ◽  
Inhibitory Effect ◽  
Hepatocyte Growth

PDGF-AA and its receptor influence early lung branching via an epithelial-mesenchymal interaction

Development ◽  
1995 ◽  
Vol 121 (8) ◽  
pp. 2559-2567 ◽  
Author(s):  
P. Souza ◽  
M. Kuliszewski ◽  
J. Wang ◽  
I. Tseu ◽  
A.K. Tanswell ◽  
...  
Keyword(s):  
Dna Synthesis ◽  
Critical Role ◽  
Polymerase Chain Reaction Analysis ◽  
Branching Morphogenesis ◽  
Inhibitory Effect ◽  
Embryonic Lung ◽  
Morphometric Analyses ◽  
Terminal Buds ◽  
Lung Branching

The biological role of platelet-derived growth factor (PDGF)-AA in lung morphogenesis was investigated by incubating embryonic lung explants with phosphorothioate antisense PDGF-A oligonucleotides, which decreased PDGF-AA but not PDGF-BB protein content. Antisense PDGF-A oligonucleotides inhibited DNA synthesis. This inhibitory effect of antisense PDGF-A was reversed by the addition of exogenous PDGF-AA but not PDGF-BB. Morphometric analyses of antisense-treated cultures showed a significant reduction in lung size. The number of terminal buds of the lung explants was significantly decreased by antisense PDGF-A oligonucleotides. PDGF-AA but not PDGF-BB attenuated the inhibitory effect of antisense PDGF-A on early lung branching. Sense PDGF-A had no effect on DNA synthesis and early lung branching. Reverse transcriptase-polymerase chain reaction analysis revealed PDGF-A mRNA expression in the epithelial component of the embryonic lung, while message for PDGF alpha-receptor was expressed in the mesenchyme. Incubation of explants with neutralizing PDGF-AA antibodies also reduced DNA synthesis and early branching morphogenesis. We conclude that PDGF-AA and its receptor represent an important epithelial-mesenchymal interaction which plays a critical role in early lung branching morphogenesis.

The calcium signal for BALB/MK keratinocyte terminal differentiation counteracts epidermal growth factor (EGF) very early in the EGF-induced proliferative pathway

1988 ◽  
Vol 8 (2) ◽  
pp. 557-563
Author(s):  
P P Di Fiore ◽  
J Falco ◽  
I Borrello ◽  
B Weissman ◽  
S A Aaronson
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Dna Synthesis ◽  
Fold Increase ◽  
Inhibitory Effect ◽  
Lymphoid Cells ◽  
Epidermal Keratinocytes ◽  
Calcium Signal ◽  
High Calcium ◽  
Epidermal Growth

BALB/MK mouse epidermal keratinocytes require epidermal growth factor (EGF) for proliferation and terminally differentiate in response to high calcium concentrations. We show that EGF is an extremely potent mitogen, causing BALB/MK cultures to enter the cell cycle in a synchronous manner associated with a greater than 100-fold increase in DNA synthesis. Analysis of the expression of proto-oncogenes which have been reported to be activated during the cascade of events following growth factor stimulation of fibroblasts or lymphoid cells revealed a very rapid but transient 100-fold increase in c-fos RNA but little or no effect on the other proto-oncogenes analyzed. Exposure of EGF-synchronized BALB/MK cells to high levels of calcium was associated with a striking decrease in the early burst of c-fos RNA as well as the subsequent peak of cell DNA synthesis. Since the inhibitory effect of high calcium on c-fos RNA expression was measurable within 30 min, our studies imply that the EGF proliferative and calcium differentiation signals must interact very early in the pathway of EGF-induced proliferation. Our results also establish that c-fos RNA modulation is an important early marker of cell proliferation in epithelial as well as mesenchymal cells.

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