A procedure for plant regeneration from immature cotyledon tissue of soybean

1985 ◽  
Vol 3 (4) ◽  
pp. 160-168 ◽  
Author(s):  
Paul A. Lazzeri ◽  
David F. Hildebrand ◽  
Glenn B. Collins
Keyword(s):  
Plant Regeneration ◽  
Immature Cotyledon

Plant regeneration from protoplasts of sugar beet (Beta vulgaris)

1995 ◽  
Vol 94 (2) ◽  
pp. 342-350 ◽  
Author(s):  
Steffen Lenzner ◽  
Kurt Zoglauer ◽  
Otto Schieder
Keyword(s):  
Plant Regeneration ◽  
Sugar Beet ◽  
Beta Vulgaris

Callus Induction and Plant Regeneration ofRosa hybrida

2014 ◽  
Vol 49 (5) ◽  
pp. 595 ◽  
Author(s):  
Feng Huan ◽  
Yi Shuli ◽  
Xie Jiaheng ◽  
Lei Mengqi ◽  
Huang Xuan
Keyword(s):  
Plant Regeneration ◽  
Callus Induction

Embryogenesis and Plant Regeneration from Unpollinated Ovary Culture of Lily

2015 ◽  
Vol 50 (3) ◽  
pp. 378
Author(s):  
Yuan Suxia ◽  
Li Jia ◽  
Ming Jun ◽  
Liu Chun ◽  
Xu Leifeng ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Ovary Culture

Plant Regeneration from Excised Cotyledons of Mature Strawberry Achenes

HortScience ◽  
1990 ◽  
Vol 25 (5) ◽  
pp. 569-571 ◽  
Author(s):  
A. Raymond Miller ◽  
Craig K. Chandler
Keyword(s):  
Acetic Acid ◽  
Plant Regeneration ◽  
Multiple Shoot ◽  
Naphthaleneacetic Acid ◽  
Developmental Studies ◽  
Shoot Buds ◽  
Cotyledon Explants ◽  
Multiple Shoot Buds ◽  
Rapid Regeneration ◽  
Dichlorophenoxy Acetic Acid

A protocol was developed for excising and culturing cotyledon explants from mature achenes of strawberry (Fragaria × ananassa Duch.). Cotyledon explants formed callus with multiple shoot buds on agar-solidified Murashige and Skoog media containing several combinations of hormones (1 μm 2,4-D; 10 μm 2,4-D; 1 μm BA + 1 μm 2,4-D; 1 μm BA + 10 μm 2,4-D; 5 μm BA; 5 μm BA + 1 μm 2,4-D; 5 μm BA + 10 μ m 2,4-D; 5 μ m BA + 5 μm NAA; 5 μ m BA + 15 μ m NAA). After three subcultures, only tissues maintained on the medium containing 5 μm BA + 5 μm NAA continued to form shoots. Tissues transferred to other media eventually died (1 μm 2,4-D; 1 μ m BA + 10 μ m 2,4-D; 5 μ m BA; 5 μ m BA + 1 μ m 2,4-D), became unorganized (1 μm BA + 1 μm 2,4-D; 5 μm BA + 10 μm 2,4-D; 5 μm BA + 15 μm NAA), or formed roots (10 μm 2,4-D). Whole plantlets were produced by transferring callus with buds to medium lacking hormones. The rapid regeneration of clonal plantlets from cotyledon explants may be useful for reducing variability in future developmental studies. Chemical names used: N-(phenylmethyl)-1H-purin-6-amine (BA); (2,4-dichlorophenoxy) acetic acid (2,4-D); and 1-naphthaleneacetic acid (NAA).

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