Rapid and efficient method for isolating plant high molecular weight (HMW) DNA with high purity

1999 ◽  
Vol 44 (4) ◽  
pp. 378-380 ◽  
Author(s):  
Fang Qiu ◽  
Jianmin Fu ◽  
Weiwu Xie ◽  
Chuanyou Li ◽  
Bin Wang
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Efficient Method ◽  
High Purity ◽  
Hmw Dna

THE APPLICATION OF THE ARNDT–EISTERT REACTION TO THE SYNTHESIS OF FATTY ACIDS OF HIGH MOLECULAR WEIGHT

1950 ◽  
Vol 28b (9) ◽  
pp. 556-560 ◽  
Author(s):  
F. A. Vandenheuvel ◽  
P. Yates
Keyword(s):  
Fatty Acids ◽  
Molecular Weight ◽  
Carboxylic Acid ◽  
Stearic Acid ◽  
High Molecular Weight ◽  
Efficient Method ◽  
Convenient Method ◽  
Ultraviolet Absorption ◽  
Aliphatic Carboxylic Acid ◽  
Eicosanoic Acid

The Arndt–Eistert reaction offers a convenient method for the synthesis of the higher members of the aliphatic carboxylic acid series. Nonadecanoic acid, eicosanoic acid, and heneicosanoic acid have been prepared successively from stearic acid in good yields. An efficient method of purification of the synthetic products is described. The ultraviolet absorption maxima for some diazoketones derived from the higher members of the aliphatic carboxylic acid series are recorded.

scholarly journals A simple and efficient method for isolating high molecular weight DNA from mammalian sperm

1988 ◽  
Vol 16 (3) ◽  
pp. 1208-1208 ◽  
Author(s):  
B.R. Bahnak ◽  
Q.Y. Wu ◽  
L. Coulombel ◽  
L. Drouet ◽  
D. Kerbiriou-Nabias ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Efficient Method ◽  
High Molecular Weight Dna ◽  
Mammalian Sperm

scholarly journals Isolation of High Molecular Weight DNA from the Model Beetle Tribolium for Nanopore Sequencing

Genes ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 1114
Author(s):  
Marin Volarić ◽  
Damira Veseljak ◽  
Brankica Mravinac ◽  
Nevenka Meštrović ◽  
Evelin Despot-Slade
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Critical Factor ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Total Output ◽  
Nanopore Sequencing ◽  
High Molecular Weight Dna ◽  
Long Read ◽  
Hmw Dna

The long-read Nanopore sequencing has been recently applied for assembly of complex genomes and analysis of linear genome organization. The most critical factor for successful long-read sequencing is extraction of high molecular weight (HMW) DNA of sufficient purity and quantity. The challenges associated with input DNA quality are further amplified when working with extremely small insects with hard exoskeletons. Here, we optimized the isolation of HMW DNA from the model beetle Tribolium and tested for use in Nanopore sequencing. We succeeded in overcoming all the difficulties in HMW handling and library preparation that were encountered when using published protocols and commercial kits. Isolation of nuclei and subsequent purification of DNA on an anion-exchange chromatography column resulted in genomic HMW DNA that was efficiently relaxed, of optimal quality and in sufficient quantity for Nanopore MinION sequencing. DNA shearing increased average N50 read values up to 26 kb and allowed us to use a single flow cell in multiple library loads for a total output of more than 13 Gb. Although our focus was on T. castaneum and closely related species, we expect that this protocol, with appropriate modifications, could be extended to other insects, particularly beetles.

Abstract 1704: AC electrokinetic isolation of cell free high molecular weight DNA (CF-HMW DNA) from serum

2012 ◽  
Author(s):  
Jerry Lu ◽  
William Hanna ◽  
Lucas S. Kumosa ◽  
Megha Bhalla ◽  
Veronica W. Cheung ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
High Molecular Weight Dna ◽  
Hmw Dna

scholarly journals When Attempting Chain Extension, Even Without Solvent, It Is Not Possible to Avoid Chojnowski Metathesis Giving D3

Molecules ◽  
2021 ◽  
Vol 26 (1) ◽  
pp. 231
Author(s):  
Mengchen Liao ◽  
Yang Chen ◽  
Michael A. Brook
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Efficient Method ◽  
Heterogeneous Reaction ◽  
Chain Extension ◽  
Molecular Weights ◽  
Second Growth ◽  
The One ◽  
Hydrolysis Of ◽  
Homogeneous Reactions

A simple, mild and efficient method to prepare HSi- or HOSi-telechelic, high-molecular-weight polydimethylsiloxane polymers (to 41,600 g·mol−1) using the one-shot hydrolysis of MHMH is reported; titration of the water allowed for higher molecular weights (to 153,900 g·mol−1). The “living” character of the chain extension processes was demonstrated by adding a small portion of MHMH and B(C6F5)3 (BCF) to a first formed polymer, which led to a ~2-fold, second growth in molecular weight. The heterogeneous reaction reached completion in less than 30 min, much less in some cases, regardless of whether it was performed neat or 50 wt% in dry toluene; homogeneous reactions in toluene were much slower. The process does not involve traditional redistribution, as judged by the low quantities (<3%) of D4 produced. However, it is not possible to avoid Chojnowski metathesis from MHDDMH giving D3, which occurs competitively with chain extension.

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