Experimental study on allogenic decalcified bone matrix as carrier for bone tissue engineering

2004 ◽  
Vol 24 (2) ◽  
pp. 147-150 ◽  
Author(s):  
Zheng Dong ◽  
Yang Shuhua ◽  
Li Jin ◽  
Xu Weihua ◽  
Yang Cao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Experimental Study ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Matrix ◽  
Decalcified Bone Matrix

scholarly journals A novel gelatin/chitooligosaccharide/demineralized bone matrix composite scaffold and periosteum-derived mesenchymal stem cells for bone tissue engineering

2021 ◽  
Vol 25 (1) ◽  
Author(s):  
Thakoon Thitiset ◽  
Siriporn Damrongsakkul ◽  
Supansa Yodmuang ◽  
Wilairat Leeanansaksiri ◽  
Jirun Apinun ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Alp Activity

Abstract Background A novel biodegradable scaffold including gelatin (G), chitooligosaccharide (COS), and demineralized bone matrix (DBM) could play a significant part in bone tissue engineering. The present study aimed to investigate the biological characteristics of composite scaffolds in combination of G, COS, and DBM for in vitro cell culture and in vivo animal bioassays. Methods Three-dimensional scaffolds from the mixture of G, COS, and DBM were fabricated into 3 groups, namely, G, GC, and GCD using a lyophilization technique. The scaffolds were cultured with mesenchymal stem cells (MSCs) for 4 weeks to determine biological responses such as cell attachment and cell proliferation, alkaline phosphatase (ALP) activity, calcium deposition, cell morphology, and cell surface elemental composition. For the in vivo bioassay, G, GC, and GCD, acellular scaffolds were implanted subcutaneously in 8-week-old male Wistar rats for 4 weeks and 8 weeks. The explants were assessed for new bone formation using hematoxylin and eosin (H&E) staining and von Kossa staining. Results The MSCs could attach and proliferate on all three groups of scaffolds. Interestingly, the ALP activity of MSCs reached the greatest value on day 7 after cultured on the scaffolds, whereas the calcium assay displayed the highest level of calcium in MSCs on day 28. Furthermore, weight percentages of calcium and phosphorus on the surface of MSCs after cultivation on the GCD scaffolds increased when compared to those on other scaffolds. The scanning electron microscopy images showed that MSCs attached and proliferated on the scaffold surface thoroughly over the cultivation time. Mineral crystal aggregation was evident in GC and greatly in GCD scaffolds. H&E staining illustrated that G, GC, and GCD scaffolds displayed osteoid after 4 weeks of implantation and von Kossa staining confirmed the mineralization at 8 weeks in G, GC, and GCD scaffolds. Conclusion The MSCs cultured in GCD scaffolds revealed greater osteogenic differentiation than those cultured in G and GC scaffolds. Additionally, the G, GC, and GCD scaffolds could promote in vivo ectopic bone formation in rat model. The GCD scaffolds exhibited maximum osteoinductive capability compared with others and may be potentially used for bone regeneration.

Osteogenesis and Degradability of Bioresorbable Biphasic Gypsum-Carbonated Apatite Granules for Bone Tissue Engineering

2016 ◽  
Vol 705 ◽  
pp. 297-303
Author(s):  
Shirin Ibrahim ◽  
Syazana Abu Bakar ◽  
Mohamad Azmirruddin Ahmad ◽  
Nurul Awanis Johan ◽  
Siti Farhana Hisham ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Weight Loss ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Matrix ◽  
Apatite Formation ◽  
Potential Candidate ◽  
Alp Activity ◽  
Carbonated Apatite

Osteogenesis and degradability of bioresorbable biphasic gypsum-carbonated apatite granules (BPG) were investigated. Three different sizes of gypsum, 300-600 μm (small), 600-1000 μm (medium) and 1000-2000 μm (large), denoted as S, M and L respectively, were developed through the crushing and sieving method. Exposure of gypsum granules in carbonate and phosphate sources formed BPG through dissolution and precipitation mechanism. BPG was firstly examined by X-ray Diffractometer (XRD) and Fourier Transform Infrared Spectrometer (FTIR) to confirm its phase and chemical composition respectively. In-vitro cell proliferation, alkaline phosphatase (ALP) activity and adhesion of human osteoblast (hFOB) were investigated for osteogenesis evaluation. Degradability in phosphate buffer saline (PBS) was characterized by weight loss whereas apatite mineralization on the BPG surface was examined using Scanning Electron Microscope (SEM). BPG with 300-600 μm and 600-1000 μm enhanced osteogenic differentiation of hFOB and accelerated differentiation process better than 1000-2000 μm as indicated by cell proliferation and ALP activity. Good hFOB adhesion was observed on all BPG surfaces. The weight loss of L and M was 68% and 59%, respectively, which are higher than S at only 32%, indicating faster degradation of large BPG compared to smaller granules upon immersion for 35 days. This in turn, suggested the ionic dissolution of BPG which has contributed to the apatite formation on its surface. The results suggest, the BPG mimicked the bone matrix, exhibited good osteogenesis and degradability, which might be used as a potential candidate for bone tissue engineering.

Optimization of scaffold design for bone tissue engineering: A computational and experimental study

2014 ◽  
Vol 36 (4) ◽  
pp. 448-457 ◽  
Author(s):  
Marta R. Dias ◽  
José M. Guedes ◽  
Colleen L. Flanagan ◽  
Scott J. Hollister ◽  
Paulo R. Fernandes
Keyword(s):  
Tissue Engineering ◽  
Experimental Study ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Scaffold Design

Bone Tissue Engineering for Spine Fusion: An Experimental Study on Ectopic and Orthotopic Implants in Rats

2004 ◽  
Vol 10 (1-2) ◽  
pp. 231-239 ◽  
Author(s):  
S.M. Van Gaalen ◽  
W.J.A. Dhert ◽  
A. Van Den Muysenberg ◽  
F.C. Oner ◽  
C. Van Blitterswijk ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Experimental Study ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Spine Fusion

scholarly journals Osteoinductive potential of small intestinal submucosa/ demineralized bone matrix as composite scaffolds for bone tissue engineering

2010 ◽  
Vol 4 (6) ◽  
pp. 913-922 ◽  
Author(s):  
Sittisak Honsawek ◽  
Piyanuch Bumrungpanichthaworn ◽  
Voranuch Thanakit ◽  
Vachiraporn Kunrangseesomboon ◽  
Supamongkon Muchmee ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Formation ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Wistar Rat ◽  
New Bone Formation ◽  
Osteoinductive Potential ◽  
Intestinal Submucosa

Abstract Background: Demineralized bone matrix (DBM) is extensively used in orthopedic, periodontal, and maxillofacial application and investigated as a material to induce new bone formation. Small intestinal submucosa (SIS) derived from the submucosa layer of porcine intestine has widely utilized as biomaterial with minimum immune response. Objectives: Determine the osteoinductive potential of SIS, DBM, SIS/DBM composites in the in vitro cell culture and in vivo animal bioassays for bone tissue engineering. Materials and methods: Human periosteal (HPO) cells were treated in the absence or presence SIS, DBM, and SIS/DBM. Cell proliferation was examined by direct cell counting. Osteoblast differentiation of the HPO cells was analyzed with alkaline phosphatase activity assay. The Wistar rat muscle implant model was used to evaluate the osteoinductive potential of SIS, DBM, and SIS/DBM composites. Results: HPO cells could differentiate along osteogenic lineage when treated with either DBM or SIS/DBM. SIS/ DBM had a tendency to promote more cellular proliferation and osteoblast differentiation than the other treatments. In Wistar rat bioassay, SIS showed no new bone formation and the implants were surrounded by fibrous tissues. DBM demonstrated new bone formation along the edge of old DBM particles. SIS/DBM composite exhibited high osteoinductivity, and the residual SIS/DBM was surrounded by osteoid-like matrix and newly formed bone. Conclusion: DBM and SIS/DBM composites could retain their osteoinductive capability. SIS/DBM scaffolds may provide an alternative approach for bone tissue engineering.

scholarly journals Material-Dependent Formation and Degradation of Bone Matrix—Comparison of Two Cryogels

2020 ◽  
Vol 7 (2) ◽  
pp. 52 ◽  
Author(s):  
Weidong Weng ◽  
Victor Häussling ◽  
Romina H. Aspera-Werz ◽  
Fabian Springer ◽  
Helen Rinderknecht ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Mineral Content ◽  
Platelet Rich Plasma ◽  
Calcium Phosphates ◽  
Bone Matrix ◽  
Osteogenic Potential ◽  
Toxic Substances ◽  
The Matrix

Cryogels represent ideal carriers for bone tissue engineering. We recently described the osteogenic potential of cryogels with different protein additives, e.g., platelet-rich plasma (PRP). However, these scaffolds raised concerns as different toxic substances are required for their preparation. Therefore, we developed another gelatin (GEL)-based cryogel. This study aimed to compare the two scaffolds regarding their physical characteristics and their influence on osteogenic and osteoclastic cells. Compared to the PRP scaffolds, GEL scaffolds had both larger pores and thicker walls, resulting in a lower connective density. PRP scaffolds, with crystalized calcium phosphates on the surface, were significantly stiffer but less mineralized than GEL scaffolds with hydroxyapatite incorporated within the matrix. The GEL scaffolds favored adherence and proliferation of the osteogenic SCP-1 and SaOS-2 cells. Macrophage colony-stimulating factor (M-CSF) and osteoprotegerin (OPG) levels seemed to be induced by GEL scaffolds. Levels of other osteoblast and osteoclast markers were comparable between the two scaffolds. After 14 days, mineral content and stiffness of the cryogels were increased by SCP-1 and SaOS-2 cells, especially of PRP scaffolds. THP-1 cell-derived osteoclastic cells only reduced mineral content and stiffness of PRP cryogels. In summary, both scaffolds present powerful advantages; however, the possibility to altered mineral content and stiffness may be decisive when it comes to using PRP or GEL scaffolds for bone tissue engineering.

scholarly journals Impact of Four Protein Additives in Cryogels on Osteogenic Differentiation of Adipose-Derived Mesenchymal Stem Cells

2019 ◽  
Vol 6 (3) ◽  
pp. 67 ◽  
Author(s):  
Victor Häussling ◽  
Sebastian Deninger ◽  
Laura Vidoni ◽  
Helen Rinderknecht ◽  
Marc Ruoß ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cell Attachment ◽  
Platelet Rich Plasma ◽  
Bone Matrix ◽  
Survival Rates ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Rgd Peptides

Human adipose-derived mesenchymal stem/stromal cells (Ad-MSCs) have great potential for bone tissue engineering. Cryogels, mimicking the three-dimensional structure of spongy bone, represent ideal carriers for these cells. We developed poly(2-hydroxyethyl methacrylate) cryogels, containing hydroxyapatite to mimic inorganic bone matrix. Cryogels were additionally supplemented with different types of proteins, namely collagen (Coll), platelet-rich plasma (PRP), immune cells-conditioned medium (CM), and RGD peptides (RGD). The different protein components did not affect scaffolds’ porosity or water-uptake capacity, but altered pore size and stiffness. Stiffness was highest in scaffolds with PRP (82.3 kPa), followed by Coll (55.3 kPa), CM (45.6 kPa), and RGD (32.8 kPa). Scaffolds with PRP, CM, and Coll had the largest pore diameters (~60 µm). Ad-MSCs were osteogenically differentiated on these scaffolds for 14 days. Cell attachment and survival rates were comparable for all four scaffolds. Runx2 and osteocalcin levels only increased in Ad-MSCs on Coll, PRP and CM cryogels. Osterix levels increased slightly in Ad-MSCs differentiated on Coll and PRP cryogels. With differentiation alkaline phosphatase activity decreased under all four conditions. In summary, besides Coll cryogel our PRP cryogel constitutes as an especially suitable carrier for bone tissue engineering. This is of special interest, as this scaffold can be generated with patients’ PRP.

Heparin-Chitosan-Coated Acellular Bone Matrix Enhances Perfusion of Blood and Vascularization in Bone Tissue Engineering Scaffolds

2011 ◽  
Vol 17 (19-20) ◽  
pp. 2369-2378 ◽  
Author(s):  
Xin-jun Sun ◽  
Wei Peng ◽  
Zai-liang Yang ◽  
Ming-liang Ren ◽  
Shi-chang Zhang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Matrix ◽  
Tissue Engineering Scaffolds
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