Expressing activity of promoter elements of large intergenic region from cotton leaf curl virus in host plant

2001 ◽  
Vol 44 (1) ◽  
pp. 8-17 ◽  
Author(s):  
Yingqiu Xie ◽  
Yule Liu ◽  
Zhen Zhu
Keyword(s):  
Host Plant ◽  
Intergenic Region ◽  
Cotton Leaf ◽  
Promoter Elements ◽  
Cotton Leaf Curl Virus ◽  
Leaf Curl Virus ◽  
Large Intergenic Region ◽  
Leaf Curl

scholarly journals Development of Cotton leaf curl virus resistant transgenic cotton using antisense ßC1 gene

2016 ◽  
Vol 23 (3) ◽  
pp. 358-362 ◽  
Author(s):  
Sayed Sartaj Sohrab ◽  
Mohammad A. Kamal ◽  
Abdul Ilah ◽  
Azamal Husen ◽  
P.S. Bhattacharya ◽  
...  
Keyword(s):  
Transgenic Cotton ◽  
Cotton Leaf ◽  
Cotton Leaf Curl Virus ◽  
Leaf Curl Virus ◽  
Leaf Curl

scholarly journals Exploration of Cotton Leaf Curl Virus (CLCuV) resistance genes and their screening in Gossypium arboreum by targeting resistance gene analogues

AoB Plants ◽  
2018 ◽  
Author(s):  
Rakhshanda Mushtaq ◽  
Khurram Shahzad ◽  
Shahid Mansoor ◽  
Zahid Hussain Shah ◽  
Hameed Alsamadany ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Resistance Genes ◽  
Cotton Leaf ◽  
Gossypium Arboreum ◽  
Cotton Leaf Curl Virus ◽  
Leaf Curl Virus ◽  
Leaf Curl

scholarly journals Widespread Occurrence of Cotton leaf curl virus on Radish in Pakistan

Plant Disease ◽  
2000 ◽  
Vol 84 (7) ◽  
pp. 809-809 ◽  
Author(s):  
S. Mansoor ◽  
S. Mukhtar ◽  
M. Hussain ◽  
I. Amin ◽  
Y. Zafar ◽  
...  
Keyword(s):  
Host Range ◽  
Full Length ◽  
Cotton Leaf ◽  
Degenerate Primers ◽  
Nylon Membrane ◽  
Punjab Province ◽  
Cotton Leaf Curl Virus ◽  
Kitchen Gardens ◽  
Leaf Curl Virus ◽  
Leaf Curl

The current epidemic of cotton leaf curl disease (CLCuD) in Pakistan started in 1988 with the natural host range limited to a few plant species in the family Malvaceae. However, we have observed expansion in the host range of the virus, and several non-Malvaceous plants were found to be infected with the virus. Characteristic symptoms of CLCuD such as leaf curl and enations have been observed on radish plants, primarily in kitchen gardens. However, in 1999, levels of infection of 10 to 90% were observed both in commercial fields and kitchen gardens in the Punjab province of Pakistan. Both symptomatic and nonsymptomatic samples were collected from five different locations. Total DNA was isolated, dot-blotted on nylon membrane, and a full-length clone corresponding to DNA A of cotton leaf curl virus was labeled with 32P dCTP and used as a probe for the detection of a begomovirus. Strong signals were observed in symptomatic plants while no signals were observed in nonsymptomatic plants. Infection with a begomovirus was further confirmed by polymerase chain reaction (PCR) using degenerate primers for DNA A (1). Primers specific for the two distinct begomoviruses associated with CLCuD were also used in PCR reactions (2), and products of the expected size were obtained from all symptomatic samples, confirming infection with begomoviruses similar to those associated with CLCuD. A full-length probe of a nanovirus-like molecule associated with cotton leaf disease (3), called DNA 1 was labeled with 32P dCTP and detected the virus only in symptomatic plants. Similarly, primers specific for DNA 1 (3) amplified a product of expected size when used in PCR. On the basis of symptomatology and the detection of specific viral components associated with the disease, we confirmed that radish plants are infected with Cotton leaf curl virus (CLCuV). Since radish is a short duration crop, infection of CLCuV in radish may not serve as a direct source of infection for the next cotton crop. However, it is a potential threat to tomato crops which overlap with radish in the Punjab province. The detection of CLCuD in radish is another example of the mobilization of begomoviruses to previously unknown hosts. References: (1) M. R. Rojas et al. Plant Dis. 77:340, 1993. (2) S. Mansoor et al. Pak. J. Bot. 31:115, 1999. (3) Mansoor et al. Virology 259:190, 1999.

Testing of Cotton Leaf Curl Virus Resistance of Candidate Varieties/strains Through Different Techniques

2002 ◽  
Vol 1 (4) ◽  
pp. 489-491
Author(s):  
Tariq Mahmood ◽  
Muhammad Tahir . ◽  
Hafiz Tariq Mahmood . ◽  
Sabahat Hussain .
Keyword(s):  
Virus Resistance ◽  
Cotton Leaf ◽  
Cotton Leaf Curl Virus ◽  
Leaf Curl Virus ◽  
Leaf Curl

scholarly journals Inheritance of resistance to Cotton leaf curl virus in cotton (Gossypium hirsutum L.)

2008 ◽  
Vol 43 (No. 1) ◽  
pp. 5-9 ◽  
Author(s):  
I. Khan A ◽  
M. Hussain ◽  
S. Rauf ◽  
M. Khan T
Keyword(s):  
Single Gene ◽  
Cotton Leaf ◽  
Breeding Method ◽  
Narrow Sense Heritability ◽  
Additive Variance ◽  
Gene Effects ◽  
Genetic Components ◽  
Cotton Leaf Curl Virus ◽  
Leaf Curl Virus ◽  
Leaf Curl

Resistance to <i>Cotton leaf curl virus</i> (CLCuV) in three cultivars of cotton was investigated in crosses with a susceptible cultivar using generation mean analysis. No single gene of major effect controlled resistance to Cotton leaf curl virus in the three crosses. The mean number of effective factors controlling resistance in cross LRA-5166 &times; S-12 was estimated to be at least five. Estimates of broad and narrow sense heritability indicate that effects by the environment were larger than those of genetic components. Epistasis was significant in two crosses. Additive gene effects contributed more to resistance than to susceptibility in contrast with dominance gene effect. Reciprocal differences were detected in the cross with LRA-5166. Estimates of genetic gain ranged form low to moderate. Thus, a breeding method that makes use of additive variance should be used because much of the variances for resistance are additive, whereas dominance effects, at least in these crosses, tended to contribute to susceptibility.

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