Species relationships between a wild tetraploid potato species,Solanum acaule bitter, and its related species as revealed by rflps of chloroplast and nuclear DNA

2002 ◽  
Vol 79 (2) ◽  
pp. 85-98 ◽  
Author(s):  
Kumi Nakagawa ◽  
Kazuyoshi Hosaka
1998 ◽  
Vol 18 (1-2) ◽  
pp. 89-93 ◽  
Author(s):  
V.-M. Rokka ◽  
C. A. Ishimaru ◽  
N. L. V. Lapitan ◽  
E. Pehu

Author(s):  
Jinping Zhao ◽  
Haolang Jiang ◽  
Guanyu Wang ◽  
Zonghua Wang ◽  
Jingao Dong ◽  
...  

Zootaxa ◽  
2007 ◽  
Vol 1562 (1) ◽  
pp. 43-53
Author(s):  
MOHSEN MOFIDI-NEYESTANAK ◽  
DONALD L.J. QUICKE

Eupholidoptera karatolosi Mofidi-Neyestanak & Quicke from Greece and E. mirzayani Mofidi-Neyestanak & Quicke from Iran, two new species of bushcrickets (Orthoptera, Tettigoniidae: Platycleidini), are described and distinguished from closely related species based on morphology. Species relationships within Eupholidoptera Maran are discussed and some species are given new assignments. A simplified illustrated identification key to the species of Eupholidoptera is provided to accommodate the new species. They are being described since they have been used to generate DNA sequence data that will be published elsewhere as part of a phylogenetic study of the tribe Platycleidini.


2016 ◽  
Author(s):  
Sereina Rutschmann ◽  
Harald Detering ◽  
Sabrina Simon ◽  
Jakob Fredslund ◽  
Michael T. Monaghan

AbstractHigh-throughput sequencing has laid the foundation for fast and cost-effective development of phylogenetic markers. Here we present the program DISCOMARK, which streamlines the development of nuclear DNA (nDNA) markers from whole-genome (or whole-transcriptome) sequencing data, combining local alignment, alignment trimming, reference mapping and primer design based on multiple sequence alignments in order to design primer pairs from input orthologous sequences. In order to demonstrate the suitability of DISCOMARK we designed markers for two groups of species, one consisting of closely related species and one group of distantly related species. For the closely related members of the species complex of Cloeon dipterum s.l. (Insecta, Ephemeroptera), the program discovered a total of 78 markers. Among these, we selected eight markers for amplification and Sanger sequencing. The exon sequence alignments (2,526 base pairs (bp)) were used to reconstruct a well supported phylogeny and to infer clearly structured haplotype networks. For the distantly related species we designed primers for several families in the insect order Ephemeroptera, using available genomic data from four sequenced species. We developed primer pairs for 23 markers that are designed to amplify across several families. The DISCOMARK program will enhance the development of new nDNA markersby providing a streamlined, automated approach to perform genome-scale scans for phylogenetic markers. The program is written in Python, released under a public license (GNU GPL v2), and together with a manual and example data set available at: https://github.com/hdetering/discomark.


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