Nuclear reconstitution of demembranatedOrychophragmus violaceus sperm inXenopus laevis egg extracts

2002 ◽  
Vol 45 (6) ◽  
pp. 623-630
Author(s):  
Ping Lu ◽  
Min Ren ◽  
Zhonghe Zhai
Keyword(s):  
Egg Extracts ◽  
Inxenopus Laevis ◽  
Nuclear Reconstitution

scholarly journals Isolation and developmental expression ofMitf inXenopus laevis

2004 ◽  
Vol 230 (1) ◽  
pp. 107-113 ◽  
Author(s):  
Mayuko Kumasaka ◽  
Hiraku Sato ◽  
Shigeru Sato ◽  
Ichiro Yajima ◽  
Hiroaki Yamamoto
Keyword(s):  
Developmental Expression ◽  
Inxenopus Laevis

scholarly journals The bulk of unpolymerized actin in Xenopus egg extracts is ATP-bound.

1995 ◽  
Vol 6 (2) ◽  
pp. 227-236 ◽  
Author(s):  
J Rosenblatt ◽  
P Peluso ◽  
T J Mitchison
Keyword(s):  
Actin Polymerization ◽  
Critical Concentration ◽  
Large Fraction ◽  
Nucleotide Exchange ◽  
Chromatography Analysis ◽  
Thymosin Beta 4 ◽  
Egg Extracts ◽  
Xenopus Egg ◽  
Xenopus Egg Extracts

Non-muscle cells contain 15-500 microM actin, a large fraction of which is unpolymerized. Thus, the concentration of unpolymerized actin is well above the critical concentration for polymerization in vitro (0.2 microM). This fraction of actin could be prevented from polymerization by being ADP bound (therefore less favored to polymerize) or by being ATP bound and sequestered by a protein such as thymosin beta 4, or both. We isolated the unpolymerized actin from Xenopus egg extracts using immobilized DNase 1 and assayed the bound nucleotide. High-pressure liquid chromatography analysis showed that the bulk of soluble actin is ATP bound. Analysis of actin-bound nucleotide exchange rates suggested the existence of two pools of unpolymerized actin, one of which exchanges nucleotide relatively rapidly and another that apparently does not exchange. Native gel electrophoresis of Xenopus egg extracts demonstrated that most of the soluble actin exists in complexes with other proteins, one of which might be thymosin beta 4. These results are consistent with actin polymerization being controlled by the sequestration and release of ATP-bound actin, and argue against nucleotide exchange playing a major role in regulating actin polymerization.

Growth and death of cells of the mesencephalic fifth nucleus inXenopus laevis larvae

1985 ◽  
Vol 233 (4) ◽  
pp. 481-489 ◽  
Author(s):  
Jerry J. Kollros ◽  
Mary L. Thiesse
Keyword(s):  
Inxenopus Laevis

scholarly journals Characterizing gene expression during lens formation inXenopus laevis: Evaluating the model for embryonic lens induction

2002 ◽  
Vol 224 (2) ◽  
pp. 168-185 ◽  
Author(s):  
Jonathan J. Henry ◽  
Maria E. Carinato ◽  
Jonathan J. Schaefer ◽  
Adam D. Wolfe ◽  
Brian E. Walter ◽  
...  
Keyword(s):  
Gene Expression ◽  
Inxenopus Laevis ◽  
Lens Formation
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