Effect of Ethephon on stomatal opening in detached epidermal strips of tobacco leaves (Nicotiana tabacum L. cv. Samsun)

1982 ◽  
Vol 24 (4) ◽  
pp. 303-306 ◽  
Author(s):  
Ingeburg Frommhold
1969 ◽  
Vol 114 (2) ◽  
pp. 331-337 ◽  
Author(s):  
A. S. Shetty ◽  
G. W. Miller

1. δ-Aminolaevulate dehydratase (EC 4.2.1.24) was purified 80-fold from tobacco leaves and its properties were studied. 2. The enzyme had optimum pH7·4 in potassium phosphate buffer, Km6·25×10−4m at 37° and pH7·4, optimum temperature 45° and an activation energy of 11100 cal./mole. 3. The enzyme lost activity when prepared in the absence of cysteine, and this activity was only partly restored by the later addition of thiols. Reagents for thiol groups inactivated the enzyme. 4. Mg2+ was essential for activity, and EDTA and Fe2+ were inhibitory; Mn2+ was an activator or an inhibitor depending on the concentration.


2008 ◽  
Vol 192 (1-4) ◽  
pp. 315-319 ◽  
Author(s):  
Nicolas Lugon-Moulin ◽  
Florian Martin ◽  
Marc R. Krauss ◽  
Patrice B. Ramey ◽  
Luca Rossi

2005 ◽  
Vol 60 (1-2) ◽  
pp. 1-4 ◽  
Author(s):  
Qun Hu ◽  
Wilhelm Boland ◽  
Ji-Kai Liu

To characterize the role of the phytotoxin mimic 6-substituted indanoyl isoleucine conjugate 1 in plant secondary metabolism, tobacco (Nicotiana tabacum L. K326) was treated with compound 1. The volatile compounds of tobacco leaves were analyzed by GC-MS. In contrast to the control, three compounds, farnesene (2), santalol (3) and tetradecanal (4), were induced by treatment with 1 mm of compound 1. Concurrently other volatile compounds were also regulated.


Author(s):  
Ning Yan ◽  
Hongbo Zhang ◽  
Zhongfeng Zhang ◽  
John Shi ◽  
Michael P. Timko ◽  
...  

Solanesol is a noncyclic terpene alcohol composed of nine isoprene units and it mainly accumulates in solanaceous plants, especially tobacco (Nicotiana tabacum L.). Here, RNA-seq analyses of tobacco leaves, stems, and roots were used to identify solanesol biosynthesis genes. Six 1-deoxy-d-xylulose 5-phosphate synthase, two 1-deoxy-d-xylulose 5-phosphate reductoisomerase, two 2-C-methyl-d-erythritol 4-phosphate cytidylyltransferase, four 4-diphosphocytidyl-2-C-methyl-d-erythritol kinase, two 2-C-methyl-d-erythritol 2,4-cyclodiphosphate synthase, four 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase, two 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase, six isopentenyl diphosphate isomerase, and two solanesyl diphosphate synthase (SPS) genes were identified to be involved in solanesol biosynthesis. Furthermore, the two N. tabacum SPS (NtSPS1 and NtSPS2), which had two conserved aspartate-rich DDxxD domains, were highly homologous with SPS enzymes from other solanaceous plant species. In addition, the solanesol contents of three organs, and leaves from four growing stages, corresponded with the distribution of chlorophyll. Our findings provide a comprehensive evaluation of the correlation between the expression of different biosynthetic genes and the accumulation of solanesol in tobacco.


Author(s):  
Sri Wahyuni Handayani ◽  
Dhian Prastowo ◽  
Hasan Boesri ◽  
Ary Oktsariyanti ◽  
Arum Sih Joharina

Dengue fever (DF) is a health problem in Indonesia. The spread of DF occurs through mosquito vectors. Vector control is one of important methods in dengue prevention. However, the occurence of insecticide resistance leads the need of new inovation of botanical insecticide, such tobacco (Nicotiana tabacum L). The research aimed to know larvicidal effectivity of tobacco extracts against Aedes aegypti larvae, and also analyzed nicotine content of tobacco leaves which collected from three sites: Semarang, Temanggung, and Kendal; used  experimental design and carried out on March-December 2013. Tobacco leaves was extracted with etanol then tested to Aedes aegypti 3rd instar larvae. The results showed that tobacco leaves from Temanggung was the most active as larvicides, then were followed from Semarang and Kendal. The analysis result showed that to reach 90% death from total number of larvae samples (LD90), required tobacco extract of Kendal at concentration 447ppm, Semarang 241 ppm, and Temanggung 212 ppm. Larvicidal effects of tobacco leaves extract was unproportional to the content of nicotine, such as Semarang (4,69%), Temanggung (3,61%), and Kendal (1,85%).


Author(s):  
Francisco Bruno S. Freire ◽  
Ricardo L.G. Bastos ◽  
Raissa S.C. Bret ◽  
Silvio A. Cândido-Sobrinho ◽  
David B. Medeiros ◽  
...  

Author(s):  
Arne J. Aasen ◽  
Sven-Olof Almquist ◽  
Curt R. Enzell

Abstract35: two isomeric 5,6-Epoxy-3-hydroxy-7-megastigmen-9-ones from Nicotiana tabacum L.


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