Amylolytic enzymes ofEndomycopsis capsularis

Fungi is known to produce a wide range of biologically active metabolites and enzymes. Enzymes produced by fungi are utilized in food and pharmaceutical industries because of their rich enzymatic profile. Filamentous fungi are particularly interesting due to their high production of extracellular enzymes which has a large industrial potential. The aim of this study is to isolate potential soil fungi species that are able to produce functional enzymes for industries. Five Aspergillus species were successfully isolated from antibiotic overexposed soil (GPS coordinate of N3.093219 E101.40269) by standard microbiological method. The isolated fungi were identified via morphological observations and molecular tools; polymerase chain reactions, ITS 1 (5’- TCC GTA GGT GAA CCT GCG G3’) forward primer and ITS 4 (5’-TCC TCC GCT TAT TGA TAT GC-3’) reverse primer. The isolated fungi were identified as Aspergillus sydowii strain SCAU066, Aspergillus tamarii isolate TN-7, Aspergillus candidus strain KUFA 0062, Aspergillus versicolor isolate BAB-6580, and Aspergillus protuberus strain KAS 6024. Supernatant obtained via submerged fermentation of the isolated fungi in potato dextrose broth (PDB) and extracted via centrifugation was loaded onto specific media to screen for the production of xylanolytic, cellulolytic and amylolytic enzymes. The present findings indicate that Aspergillus sydowii strain SCAU066 and Aspergillus versicolor isolate BAB-6580 have great potential as an alternative source of xylanolytic, cellulolytic and amylolytic enzymes.

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Linear and cyclic amylooligosaccharides and their relationship to the amylolytic enzymes

10.31274/rtd-180813-14461 ◽

1947 ◽

Author(s):

Melvin Lewis Levine

Keyword(s):

Amylolytic Enzymes

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Effect of Exogenous Amylolytic Enzymes on the Accumulation of Chlorogenic Acid Isomers in Wounded Potato Tubers

Journal of Agricultural and Food Chemistry ◽

10.1021/jf5026983 ◽

2014 ◽

Vol 62 (31) ◽

pp. 7671-7675 ◽

Cited By ~ 19

Author(s):

Ana Mariel Torres-Contreras ◽

Vimal Nair ◽

Luis Cisneros-Zevallos ◽

Daniel A. Jacobo-Velázquez

Keyword(s):

Chlorogenic Acid ◽

Potato Tubers ◽

Amylolytic Enzymes

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Sustainable Second-Generation Bioethanol Production from Enzymatically Hydrolyzed Domestic Food Waste Using Pichia anomala as Biocatalyst

Sustainability ◽

10.3390/su13010259 ◽

2020 ◽

Vol 13 (1) ◽

pp. 259

Author(s):

Ioanna Ntaikou ◽

Georgia Antonopoulou ◽

Gerasimos Lyberatos

Keyword(s):

Food Waste ◽

Second Generation ◽

Scale Up ◽

Pichia Anomala ◽

Small Scale ◽

Amylolytic Enzymes ◽

Second Generation Bioethanol ◽

Saccharification Efficiency ◽

Ethanol Yields ◽

Domestic Food

In the current study, a domestic food waste containing more than 50% of carbohydrates was assessed as feedstock to produce second-generation bioethanol. Aiming to the maximum exploitation of the carbohydrate fraction of the waste, its hydrolysis via cellulolytic and amylolytic enzymatic blends was investigated and the saccharification efficiency was assessed in each case. Fermentation experiments were performed using the non-conventional yeast Pichia anomala (Wickerhamomyces anomalus) under both separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) modes to evaluate the conversion efficiencies and ethanol yields for different enzymatic loadings. It was shown that the fermentation efficiency of the yeast was not affected by the fermentation mode and was high for all handlings, reaching 83%, whereas the enzymatic blend containing the highest amount of both cellulolytic and amylolytic enzymes led to almost complete liquefaction of the waste, resulting also in ethanol yields reaching 141.06 ± 6.81 g ethanol/kg waste (0.40 ± 0.03 g ethanol/g consumed carbohydrates). In the sequel, a scale-up fermentation experiment was performed with the highest loading of enzymes in SHF mode, from which the maximum specific growth rate, μmax, and the biomass yield, Yx/s, of the yeast from the hydrolyzed waste were estimated. The ethanol yields that were achieved were similar to those of the respective small scale experiments reaching 138.67 ± 5.69 g ethanol/kg waste (0.40 ± 0.01 g ethanol/g consumed carbohydrates).

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STUDIES IN MYOCLONUS EPILEPSY III. THE EFFECTS OF AMYLOLYTIC ENZYMES ON THE ULTRASTRUCTURE OF LAFORA BODIES

Journal of Histochemistry & Cytochemistry ◽

10.1177/19.6.382 ◽

1971 ◽

Vol 19 (6) ◽

pp. 382-385 ◽

Cited By ~ 13

Author(s):

TORU NIKAIDO ◽

JAMES AUSTIN ◽

HANS STUKENBROK

Keyword(s):

Staining Intensity ◽

Ultrastructural Changes ◽

Corpora Amylacea ◽

Amylolytic Enzymes ◽

Lafora Bodies ◽

Myoclonus Epilepsy

Sections containing Lafora bodies were exposed to the action of amylolytic enzymes. These enzymes split the glucose linkages of glucose polymers (polyglucosans). The following ultrastructural changes were observed: ( a) the fibrils of Lafora bodies were greatly reduced in number; ( b) the staining intensity of the fibrils was reduced; ( c) the amorphous densities were no longer seen. The evidence suggests that the fibrils and amorphous densities of Lafora bodies are both polyglucosan in nature. Lafora bodies resemble corpora amylacea in this respect.

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Amylolytic Enzymes

Food Enzymes ◽

10.1007/978-1-4757-2349-6_3 ◽

1995 ◽

pp. 37-84 ◽

Cited By ~ 1

Author(s):

Dominic W. S. Wong

Keyword(s):

Amylolytic Enzymes

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Production of Amylolytic Enzymes in Natural and Synthetic Media12

Applied Microbiology ◽

10.1128/am.7.4.212-218.1959 ◽

1959 ◽

Vol 7 (4) ◽

pp. 212-218

Author(s):

C. G. Dunn ◽

G. J. Fuld ◽

K. Yamada ◽

J. Mas Urioste ◽

P. R. Casey

Keyword(s):

Amylolytic Enzymes ◽

Natural And Synthetic

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The role of new stress protectors in the formation of production process of oat culture during cultivation in anthropogenically polluted areas

BIO Web of Conferences ◽

10.1051/bioconf/20202303011 ◽

2020 ◽

Vol 23 ◽

pp. 03011

Author(s):

Yuliya M. Andriyanova ◽

Irina V. Sergeyeva ◽

Nataliya N. Gusakova ◽

Yuliya M. Mokhonko

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Negative Impact ◽

Field Studies ◽

Amylolytic Enzymes ◽

Cereal Products ◽

Oat Seeds ◽

Lead Zinc

Stress protectors (adaptogens) are among the most important factors that regulate growth processes at all stages of plant development. This article presents results of field studies of the effect of new synthetic plant growth regulators of stress protectors (adaptogens) on the elements of productivity and yield of spring oats of the Skakun variety. The obtained results during the research showed that all the studied derivatives of peredazinones are adaptogens and they contribute to an increased productivity and increased yield of spring oats. We studied the effect of pre-sowing treatment of seeds with new synthetic plant growth regulators of stress protectors on the quality indicators of cereal production of Skakun oats (protein, starch and amylolytic enzymes content in the cereal). Pre-sowing treatment of oat seeds increases the amount of protein in the cereal up to 15%, starch – up to 25%, amylase – up to 20%. We proved the ability of stress protectors to minimize the negative impact of heavy metals (lead, zinc) on agrophytocenoses, which will make it possible to obtain environmentally friendly cereal products when cultivating oats in anthropogenically polluted areas of the Saratov Oblast.

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