The enhancing effect of homologous lymphoid cells on maturation of active antibody formation in newborn rabbits

1961 ◽  
Vol 6 (5) ◽  
pp. 299-305
Author(s):  
M. Holub
Keyword(s):  
Antibody Formation ◽  
Lymphoid Cells ◽  
Enhancing Effect

scholarly journals Genetic Control of the Radiosensitivity of Lymphoid Cells for Antibody Formation Ability in Mice.

1994 ◽  
Vol 35 (3) ◽  
pp. 179-185
Author(s):  
MASAAKI OKUMOTO ◽  
NOBUKO MORI ◽  
SHUNSUKE IMAI ◽  
SATOMI HAGA ◽  
JO HILGERS ◽  
...  
Keyword(s):  
Genetic Control ◽  
Antibody Formation ◽  
Lymphoid Cells

scholarly journals THE ENHANCING EFFECT OF URETHANE ON THE SEVERITY OF INFECTION WITH PNEUMONIA VIRUS OF MICE (PVM)

1952 ◽  
Vol 95 (2) ◽  
pp. 147-158 ◽  
Author(s):  
George S. Mirick ◽  
J. MacLean Smith ◽  
Charles I. Leftwich ◽  
William B. Leftwich
Keyword(s):  
Antibody Formation ◽  
Lymphoid Tissues ◽  
Enhancing Effect

Urethane, given parenterally or orally to mice, increased the severity of PVM infection. Not only were the lesions more extensive but mice could be infected with smaller inocula of virus and the multiplication of virus in the lung was enhanced. There was atrophy of lymphoid tissues but no suppression was noted of antibody formation. No relation could be found between PVM infection and the development of pulmonary adenomas in mice.

scholarly journals THE NATURE OF THE IMMUNOLOGIC INADEQUACY OF NEONATAL RABBITS

1959 ◽  
Vol 110 (1) ◽  
pp. 139-146 ◽  
Author(s):  
Frank J. Dixon ◽  
William O. Weigle
Keyword(s):  
Antibody Formation ◽  
Lymphoid Cells

It has been shown that cells from neonatal rabbits are capable of producing considerable amounts of antibody after exposure to antigen in vitro and transfer to x-radiated adult recipients. This suggests that the immunological inadequacy of these neonates is not dependent primarily upon the lack of cells capable of antibody formation. Both in vitro sensitized neonatal lymphoid cells and in vivo sensitized adult lymphoid cells made much more antibody after transfer to x-rayed adult recipients than to neonatal recipients. Possible reasons for and significance of these observations are pointed out in the discussion.

scholarly journals STUDIES ON MEMBRANE-BOUND RECEPTORS FOR ANTIGEN

1973 ◽  
Vol 138 (4) ◽  
pp. 875-886 ◽  
Author(s):  
Robert D. Stout ◽  
Albert H. Coons
Keyword(s):  
Lymphoid Cells ◽  
Cell Populations ◽  
Antigen Binding ◽  
Amino Acid Incorporation ◽  
Enhancing Effect ◽  
Optimum Range ◽  
Acid Incorporation ◽  
Rna And Protein Synthesis ◽  
Primed Cell ◽  
Membrane Bound

The effect of polyadenylic: polyundylic acid complexes (poly A:U) on the amount of antibody on the surface of various populations of mouse lymphoid cells has been investigated by means of a sensitive measure of such activity—the binding by primed cell populations of ß-galactosidase (ßGZ) as an antigen. The sensitivity derives from the liberation of fluorescein from an artificial substrate, fluorescein-di-ß-galactopyranoside (FDßG). After incubation with 100 ng/ml of poly A:U, only 40% of the cells previously showing antigen-binding were still active. The optimum range of activity lay between 0.01–1.0 µg/ml poly A:U. Such cells showed increased RNA and protein synthesis as indicated by [3H]uridine and [14C]amino acid incorporation. The polynucleotide effect was abolished by incubation of the cells with sodium azide or iodoacetate, but not by puromycin. When the proteins on the cell surface were labeled by 125I, poly A:U caused their release into the medium. Reports by others that the enhancing effect of polynucleotides on the immune response involves the adenylcyclase system are consistent with the finding reported here that reduction of binding by dibutryl 5'-cyclic monophosphoric acid (cAMP) and poly A:U were parallel in extent, and that theophylline and poly A:U acted synergistically in suboptimal concentrations of each.

scholarly journals THE EFFECTS OF MERCAPTOETHANOL AND OF PERITONEAL MACROPHAGES ON THE ANTIBODY-FORMING CAPACITY OF NONADHERENT MOUSE SPLEEN CELLS IN VITRO

1972 ◽  
Vol 136 (3) ◽  
pp. 604-617 ◽  
Author(s):  
Chang Chen ◽  
James G. Hirsch
Keyword(s):  
Antibody Formation ◽  
Peritoneal Macrophages ◽  
Lymphoid Cells ◽  
Red Cells ◽  
Mouse Spleen ◽  
Spleen Cells ◽  
Sheep Red Cells ◽  
Low Concentrations ◽  
Nonadherent Cells

Nonadherent mouse spleen cells exhibited poor viability and little or no capacity to form antibodies to sheep red cells in the Mishell-Dutton culture system. Viability and antibody-forming capacity could be restored by addition to these cultures of low concentrations of mercaptoethanol (10–4–10–5 M), or by addition of appropriate numbers of mouse peritoneal macrophages. Macrophage concentrations lower than optimal resulted in lower lymphoid cell viability and correspondingly fewer plaque-forming cells, whereas excess macrophages resulted in marked inhibition of antibody formation despite good viability of the lymphocytes. Restoration of the nonadherent cells with mercaptoethanol was thus much simpler and more reproducible than it was with macrophages; furthermore, the number of plaque-forming cells developed in cultures restored with mercaptoethanol was approximately fourfold higher than it was in cultures restored with optimal numbers of macrophages. In the presence of mercaptoethanol, the plaque-forming capacity of the nonadherent spleen cells was not increased when small numbers of macrophages were added to the system, nor was it decreased when the few macrophages present in the nonadherent cells were further reduced or eliminated. Excess macrophages inhibited antibody formation in the cultures containing mercaptoethanol as they did in control cultures. Optimal restoration of plaque-forming capacity to the nonadherent spleen cells with mercaptoethanol required the reducing agent to be present throughout the 4 or 5 day culture period. Addition of mercaptoethanol 1 or more days after initiation of culture, or transfer of the cells to a medium free of mercaptoethanol before completion of the culture resulted in a reduction in the numbers of plaque-forming cells. The results suggest that mouse lymphoid cells do not require macrophages in order to form antibodies to sheep red cells in vitro, provided mercaptoethanol is present in the culture medium. The mechanism of action of mercaptoethanol under these conditions is not completely clear, but one of its effects is to promote the viability of lymphoid cells in the cultures.

scholarly journals CARRIER FUNCTION IN ANTI-HAPTEN IMMUNE RESPONSES

1970 ◽  
Vol 132 (2) ◽  
pp. 283-299 ◽  
Author(s):  
William E. Paul ◽  
David H. Katz ◽  
Edmond A. Goidl ◽  
Baruj Benacerraf
Keyword(s):  
Immune Responses ◽  
Guinea Pigs ◽  
Preceding Paper ◽  
Lymphoid Cells ◽  
Antibody Responses ◽  
Cooperative Interaction ◽  
Antigenic Determinants ◽  
Cell Transfer ◽  
Enhancing Effect ◽  
Protein Conjugates

Transfer of live lymphoid cells from BGG-immunized strain 2 guinea pigs into syngeneic animals primed with DNP-OVA prepares the recipients for a markedly enhanced secondary anti-DNP antibody response upon challenge with DNP-BGG. This phenomenon has been demonstrated when the recipients were challenged 1 day after cell transfer, but it was considerably more striking when an interval of 6 days was allowed between transfer of cells and challenge with antigen. As demonstrated in the preceding paper (6), BGG preimmunization enhanced anti-DNP antibody responses to challenge with DNP-BGG. An analysis of the characteristics of substances to which preimmunization was effective in enhancing subsequent anti-hapten responses was made. It was shown that preimmunization of strain 13 guinea pigs with a copolymer of glutamic acid and lysine (GL), to which these animals are genetically unable to accord an immune response, failed to prepare them for an enhanced anti-DNP response to DNP-GL. Tolerance to BGG specifically abrogated the capacity of subsequent BGG immunization to prepare DNP-OVA primed rabbits for an enhanced anti-DNP response to DNP-BGG. Sensitization of animals to haptens by preimmunization with hapten-protein conjugates failed to prepare them for enhanced primary or secondary antibody responses to other determinants associated with that hapten on a different carrier. These studies indicate that the enhancing effect of carrier preimmunization reflects a cooperative interaction between lymphoid cells specific for carrier and cells specialized for haptenic determinants. Furthermore, the capacity of a substance to behave as a carrier requires more than its possession of a variety of antigenic determinants.

Lymphoproliferative disorders (LPD) involving lungs: T and B cell subsets within pulmonary infiltrates and in peripheral blood

1984 ◽  
Vol 42 ◽  
pp. 700-701
Author(s):  
Irene Stachura ◽  
Milton H. Dalbow ◽  
Michael J. Niemiec ◽  
Matias Pardo ◽  
Gurmukh Singh ◽  
...  
Keyword(s):  
B Cells ◽  
B Cell ◽  
Lymphoproliferative Disorders ◽  
Mixed Population ◽  
Lymphoid Cells ◽  
Lymphomatoid Granulomatosis ◽  
Cell Type ◽  
Cell Surface Antigens ◽  
Pulmonary Infiltrates ◽  
B Cell Subsets

Lymphoid cells were analyzed within pulmonary infiltrates of six patients with lymphoproliferative disorders involving lungs by immunofluorescence and immunoperoxidase techniques utilizing monoclonal antibodies to cell surface antigens T11 (total T), T4 (inducer/helper T), T8 (cytotoxic/suppressor T) and B1 (B cells) and the antisera against heavy (G,A,M) and light (kappa, lambda) immunoglobulin chains. Three patients had pseudolymphoma, two patients had lymphoma and one patient had lymphomatoid granulomatosis.A mixed population of cells was present in tissue infiltrates from the three patients with pseudolymphoma, IgM-kappa producing cells constituted the main B cell type in one patient. In two patients with lymphoma pattern the infiltrates were composed exclusively of T4+ cells and IgG-lambda B cells predominated slightly in the patient with lymphomatoid granulomatosis.

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