Changes in sucrose synthetase and sucrose phosphate synthetase activities during storage of potatoes

1970 ◽  
Vol 47 (7) ◽  
pp. 245-251 ◽  
Author(s):  
Russell Pressey
Keyword(s):  
Sucrose Synthetase ◽  
Sucrose Phosphate Synthetase ◽  
Sucrose Phosphate

scholarly journals The activity of uridine diphosphate glucose–d-fructose 6-phosphate 2-glucosyltransferase in leaves

1967 ◽  
Vol 105 (3) ◽  
pp. 943-946 ◽  
Author(s):  
J. S. Hawker
Keyword(s):  
Sugar Cane ◽  
Sucrose Synthesis ◽  
Uridine Diphosphate ◽  
Leaf Extracts ◽  
Uridine Diphosphate Glucose ◽  
Rate Of Photosynthesis ◽  
Diphosphate Glucose ◽  
Sucrose Phosphate Synthetase ◽  
High Sucrose ◽  
Sucrose Phosphate

1. By using EDTA in reaction mixtures it was possible to determine the activity of sucrose phosphate synthetase in freshly prepared leaf extracts without the complications caused by sucrose phosphatase. 2. EDTA was found also to increase the activity of sucrose phosphate synthetase by as much as 100%. 3. High sucrose phosphate synthetase activities were found in leaf preparations in which sucrose phosphatase was inhibited by EDTA. By contrast with previous reports, the activities were sufficient to allow sucrose synthesis in leaves during photosynthesis to occur via sucrose phosphate. 4. Sugar-cane plants having different rates of photosynthesis also had different activities of sucrose phosphate synthetase in their leaves. 5. It is suggested that the activity of sucrose phosphate synthetase in leaves may play a role in the control of the rate of photosynthesis.

A continuous spectrophotometric assay for sucrose phosphate synthetase

1980 ◽  
Vol 107 (1) ◽  
pp. 56-59 ◽  
Author(s):  
S. Harbron ◽  
I.E. Woodrow ◽  
G.J. Kelly ◽  
S.P. Robinson ◽  
E. Latzko ◽  
...  
Keyword(s):  
Spectrophotometric Assay ◽  
Sucrose Phosphate Synthetase ◽  
Sucrose Phosphate

Curing Influences Sugar Metabolizing Enzyme Activity in Sweetpotato

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 459b-459
Author(s):  
Yuehe Huang ◽  
David H. Picha ◽  
Charles E. Johnson ◽  
Anthony W. Kilili
Keyword(s):  
Enzyme Activity ◽  
Sugar Content ◽  
Invertase Activity ◽  
Breeding Line ◽  
Metabolizing Enzymes ◽  
Free Sugars ◽  
Total Sugars ◽  
Metabolizing Enzyme ◽  
Sucrose Phosphate Synthetase ◽  
Sucrose Phosphate

Sweetpotato roots (`Beauregard', `Hernandez', and LSU breeding line 9496) were cured (30 °C and 90% RH) for 10 days immediately after harvest to determine the effect of curing on several sugar-metabolizing enzymes. Free sugars and activities of invertase, sucrose synthase (SS), and sucrose phosphate synthetase (SPS) were assayed. Total sugars for the three genotypes ranged from 132.5 to 177.9 mg sugar/g DW at harvest, and from 162.3 to 204.3 mg sugar/g DW after curing. Enzyme activity at harvest averaged over the three genotypes ranged from: 154 to 251 mol sucrose DW/h for invertase; 18.6 to 23.1 mol sucrose DW/h for SS, and 7.4 to 15.7 mol sucrose DW/hr for SPS. Enzyme activity after curing averaged over the three genotypes ranged from: 251 to 288 mol sucrose DW/h for invertase; 20.3 to 25.8 mol sucrose DW/h for SS, and 11.3 to 17.3 mol sucrose DW/h for SPS. The increase in invertase activity during curing was highest in `Hernandez' and lowest in the breeding line 9496. The increase in invertase activity correlated to the increase in reducing sugar content for `Hernandez' and `Beauregard'. Curing also shifted the pH optima of invertase in `Hernandez' from pH 5.2 to 7.6.

Sucrose phosphate synthetase

Planta ◽  
1978 ◽  
Vol 142 (1) ◽  
pp. 41-48 ◽  
Author(s):  
G. L. Salerno ◽  
H. G. Pontis
Keyword(s):  
Sucrose Phosphate Synthetase ◽  
Sucrose Phosphate

scholarly journals Studies on sucrose phosphate synthetase

FEBS Letters ◽  
1978 ◽  
Vol 86 (2) ◽  
pp. 263-267 ◽  
Author(s):  
Graciela L. Salerno ◽  
Horacio G. Pontis
Keyword(s):  
Sucrose Phosphate Synthetase ◽  
Sucrose Phosphate
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