Functional genomics: Gene identification via T-DNA mediated gene trap tagging in plants

2001 ◽  
Vol 12 (1) ◽  
pp. 1-8
Author(s):  
Tang Wei ◽  
Vanessa Samuels ◽  
Janet Ogbon ◽  
Aquilla McCoy
2007 ◽  
Vol 13 (11) ◽  
pp. 771-779 ◽  
Author(s):  
Terrance Lee ◽  
Chirag Shah ◽  
Eugene Yujun Xu

Author(s):  
B. I. Je ◽  
C. M. Kim ◽  
Su Hyun Park ◽  
Sung Han Park ◽  
Y. J. Na ◽  
...  

2007 ◽  
pp. 711-717 ◽  
Author(s):  
T.N. Zhebentyayeva ◽  
D. Jiwan ◽  
J.H. Jun ◽  
D.A. Lalli ◽  
S. Forrest ◽  
...  

Methods ◽  
2005 ◽  
Vol 37 (3) ◽  
pp. 274-279 ◽  
Author(s):  
B. Bertsch ◽  
C.A. Ogden ◽  
K. Sidhu ◽  
H. Le-Niculescu ◽  
R. Kuczenski ◽  
...  

Author(s):  
Rifat Hamoudi ◽  
Sally Johnston ◽  
George Hutchinson ◽  
John D'Errico

Using a single robotic platform, the GeneTAC™ G3, we have automated most of the processes involved in the cloning and characterisation of novel disease causing genes by addressing the following; firstly, identifying the BACs of interest and making shotgun libraries. Secondly, automating the set up of sequencing reactions using methodology that eliminates the need for DNA preparation of 384 clones. Thirdly, generating sub-libraries using selective re-arraying of library clones to enable the determination of the entire genomic sequence of the gene. Fourthly, determining gene function by combination of differential screening and mini Northerns using microarrays printed using the GeneTAC™ G3 system and hybridised using the GeneTAC™ HybStation (Genomics Solutions, Ann Arbor, USA).


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