Genetic transformation of loblolly pine using mature zygotic embryo expiants byAgrobacterium tumefaciens

2000 ◽  
Vol 11 (4) ◽  
pp. 215-222
Author(s):  
Tang Wei
Keyword(s):  
Genetic Transformation ◽  
Loblolly Pine ◽  
Zygotic Embryo ◽  
Mature Zygotic Embryo

scholarly journals Plant regeneration and transformation of Trachyspermum ammi using Agrobacterium tumefaciens and zygotic embryos

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Masoumeh Nomani ◽  
Masoud Tohidfar
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Plant Regeneration ◽  
Genetic Transformation ◽  
Zygotic Embryo ◽  
Plant Biotechnology ◽  
Hplc Method ◽  
Zygotic Embryos ◽  
Naphthaleneacetic Acid ◽  
Transformed Plants ◽  
Trachyspermum Ammi

Abstract Background Trachyspermum ammi is one of the key medicinal plant species with many beneficial properties. Thymol is the most important substance in the essential oil of this plant. Thymol is a natural monoterpene phenol with high anti-microbial, anti-bacterial, and anti-oxidant properties. Thymol in the latest research has a significant impact on slowing the progression of cancer cells in human. In this research, embryos were employed as convenient explants for the fast and effectual regeneration and transformation of T. ammi. To regenerate this plant, Murashige and Skoog (MS) and Gamborg's B5 (B5) media were supplemented with diverse concentrations of plant growth regulators, such as 6-benzyladenine (BA), 1-naphthaleneacetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), and kinetin (kin). Transgenic Trachyspermum ammi plants were also obtained using Agrobacterium-mediated transformation and zygotic embryos explants. Moreover, two Agrobacterium tumefaciens strains (EHA101 and LBA4404) harboring pBI121-TPS2 were utilized for genetic transformation to Trachyspermum ammi. Results According to the obtained results, the highest plant-regeneration frequency was obtained with B5 medium supplemented with 0.5 mg/l BA and 1 mg/l NAA. The integrated gene was also approved using the PCR reaction and the Southern blot method. Results also showed that the EHA101 strain outperformed another strain in inoculation time (30 s) and co-cultivation period (1 day) (transformation efficiency 19.29%). Furthermore, HPLC method demonstrated that the transformed plants contained a higher thymol level than non-transformed plants. Conclusions In this research, a fast protocol was introduced for the regeneration and transformation of Trachyspermum ammi, using zygotic embryo explants in 25–35 days. Our findings confirmed the increase in the thymol in the aerial part of Trachyspermum ammi. We further presented an efficacious technique for enhancing thymol content in Trachyspermum ammi using Agrobacterium-mediated plant transformation system that can be beneficial in genetic transformation and other plant biotechnology techniques.

scholarly journals In vitro Propagation of Noni (Morinda citrifolia L.) Through Embryo Culture

2020 ◽  
Vol 30 (2) ◽  
pp. 199-207
Author(s):  
JO Afolabi ◽  
EO Oloyede ◽  
AO Akala
Keyword(s):  
Embryo Culture ◽  
In Vitro Propagation ◽  
Zygotic Embryo ◽  
Shoot Length ◽  
Morinda Citrifolia ◽  
Distilled Water ◽  
Number Of Leaves ◽  
And Control ◽  
Mature Zygotic Embryo

The chances of shoot-regeneration from embryo culture of Morinda citrifolia L. seeds was investigated. Germination on different strengths of MS and control (Sterile distilled water) started by two weeks after inoculation (WAI). At 6 WAI, 90% of the embryo had germinated from 25% MS followed by 80% in control, 70% from 50% MS and 40% each from 100 and 75% MS. Similarly, the same MS media strengths with basal application of 2.0/1.0 mg/l BAP/Kn affected the growth of regenerated Noni-plantlets. The longest shoot length (3.46 cm) and the number of nodes (1.75) were obtained from 75% MS while the highest number of leaves (7.25) was obtained in 100% MS between 4 and 12 WAI. The lowest value for these parameters were observed in 25% MS. This showed that mature zygotic embryo is good explant for the establishment of highly viable and re-generable plantlets of Noni. Plant Tissue Cult. & Biotech. 30(2): 199-207, 2020 (December)

scholarly journals Expression of Aintegumenta-like Gene Related to Embryogenic Competence in Coconut Confirmed by 454-pyrosequencing Transcriptome Analysis

CORD ◽  
2015 ◽  
Vol 31 (2) ◽  
pp. 11
Author(s):  
H D D Bandupriya
Keyword(s):  
Elaeis Guineensis ◽  
Zygotic Embryo ◽  
Cocos Nucifera ◽  
Zygotic Embryos ◽  
Maturity Stage ◽  
Expression Data ◽  
Rt Pcr ◽  
Embryogenic Competence ◽  
Transcript Accumulation ◽  
Mature Zygotic Embryo

A member of the Aintegumenta sub-family of Apetala gene family encoding two APETALA2 (AP2) domains was isolated and termed as Cocos nucifera Aintegumenta like gene (CnANT). The deduced amino acid sequence of the conserved domains shared a high similarity with Aintegumenta-Like (ANT like) genes in Arabidopsis thaliana, Elaeis guineensis, Oryza sativa.  Comparison of transcriptomes in different tissues revealed that CnANT transcripts were high in mature zygotic embryo (12 months after pollination; 12ME). Quantitative RT-PCR results confirmed the higher CnANT transcript accumulation in mature zygotic embryos while transcripts were rarely detected in vegetative tissues such as leaf. The expression data and global transcriptome data were therefore consistent across the embryo maturity stage and showed that CnANT could play a role in embryogenesis.

Mature zygotic embryo rescue improves in vitro germination and seedling production in high value oil palm (Elaeis guineensis Jacq.) cultivars

2016 ◽  
Vol 94 ◽  
pp. 445-453 ◽  
Author(s):  
Kingsley Tabi Mbi ◽  
Libert Brice Tonfack ◽  
Godswill Ntsomboh Ntsefong ◽  
Bilal Ahmad Mir ◽  
Georges Frank Ngando Ebongue ◽  
...  
Keyword(s):  
Oil Palm ◽  
Elaeis Guineensis ◽  
Zygotic Embryo ◽  
Embryo Rescue ◽  
In Vitro Germination ◽  
Seedling Production ◽  
Elaeis Guineensis Jacq ◽  
Mature Zygotic Embryo

scholarly journals In vitro Regeneration and Agrobacterium-mediated Genetic Transformation of Local Varieties of Mungbean (Vigna radiata (L). Wilczek)

2019 ◽  
Vol 29 (1) ◽  
pp. 81-97
Author(s):  
Sujay Kumar Bhajan ◽  
Setara Begum ◽  
Mohammad Nurul Islam ◽  
M Imdadul Hoque ◽  
Rakha Hari Sarker
Keyword(s):  
Genetic Transformation ◽  
Vigna Radiata ◽  
Zygotic Embryo ◽  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Direct Organogenesis ◽  
Pcr Analysis ◽  
Root Induction ◽  
Half Strength

An efficient Agrobacterium-mediated transformation compatible in vitro regeneration protocol was developed for two important varieties of mungbean (Vigna radiata (L.) Wilczek) cultivated in Bangladesh, namely Binamoog-5 and BARI Mung-6. Two different zygotic embryo derived explants, such as cotyledonary node (CN) and cotyledon attached decapitated embryo (CADE) were used for direct organogenesis of shoot. MS supplemented with 4.0 μM BAP was found to be the best for the development of highest number of multiple shoots from CADE in both the varieties of mungbean. While in case CN the best shoot formation was achieved on MS containing 4.0 μM BAP and 0.5 μM NAA in both varieties. Half strength of MS with 2.0 μM IBA was found to be most effective for producing healthy root from regenerated shoots. Following root induction, the in vitro raised plantlets were successfully transplanted to soil for their establishment. Considering overall responses, genetic transformation efficiency was found to be better with CADE explant using Agrobacterium tumefaciens strain LBA4404 harboring the binary plasmid pBI121 conferring GUS and nptII genes. Different factors influencing transformation was optimized during this study. Selection of transformed shoots was carried out by gradually increasing the concentration of kanamycin and such transformed shoots were eventually selected using 200 mg/l kanamycin. Stable expression of the GUS gene was detected in various parts of regenerated transformed plantlets. Transformed shoots were rooted on half strength MS containing 2.0 μM IBA and 100 mg/l ticarcillin. Rooted transformed plantlets were successfully transferred to soil. Stable integration of GUS and nptII genes in the putative transformed shoots was confirmed through PCR analysis. Plant Tissue Cult. & Biotech. 29(1): 81-97, 2019 (June)

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