Spacer length variation in rice 5S rRNA genes revealed by polymerase chain reaction

1997 ◽  
Vol 2 (1) ◽  
pp. 124-126 ◽  
Author(s):  
Yi Qingming ◽  
Liu Guoping
Keyword(s):  
Polymerase Chain Reaction ◽  
5S Rrna ◽  
Rrna Genes ◽  
Length Variation ◽  
Chain Reaction ◽  
Spacer Length ◽  
5S Rrna Genes ◽  
Polymerase Chain

scholarly journals Epidemiological Investigation ofSalmonella Tileneby Pulsed-Field Gel Electrophoresis and Polymerase Chain Reaction

1997 ◽  
Vol 8 (6) ◽  
pp. 318-322 ◽  
Author(s):  
Chandar M Anand ◽  
Kevin Fonseca ◽  
Ken Longmore ◽  
Robert Rennie ◽  
Linda Chui ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Gel Electrophoresis ◽  
Washington State ◽  
Pulsed Field Gel Electrophoresis ◽  
Dna Fingerprint ◽  
Rrna Genes ◽  
23S Rrna ◽  
Chain Reaction ◽  
Pulsed Field ◽  
Polymerase Chain

Pulsed-field gel electrophoresis (PFGE) and DNA fingerprinting by the polymerase chain reaction (PCR) were performed on 11 isolates ofSalmonella tilene. Five strains were from a cluster of human patients, six from sugar gliders and pygmy hedgehogs kept as family pets or from local pet retailers, and one isolate from the first North American case ofS tilenedescribed in Washington State in 1994. The PFGE restriction patterns showed all isolates to be similar. However, PCR using primers to the 16S and 23S rRNA genes ofEscherichia colidemonstrated that the Washington State isolate differed from the rest of the other isolates, which were all similar based upon their DNA fingerprint. This study indicates that reliance on one technique alone may be insufficient to show nuances between strains that are, in many respects, closely related.

Decontamination of polymerase chain reaction reagents for detection of low concentrations of 16S rRNA genes

1997 ◽  
Vol 7 (3) ◽  
pp. 207-216 ◽  
Author(s):  
Farida Hilali ◽  
Patrick Saulnier ◽  
Elisabeth Chachaty ◽  
Antoine Andremont
Keyword(s):  
Polymerase Chain Reaction ◽  
16S Rrna ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Chain Reaction ◽  
Low Concentrations ◽  
Polymerase Chain

Identification of Campylobacter jejuni Isolates from Cloacal and Carcass Swabs of Chickens in Thailand by a 5′Nuclease Fluorogenic Polymerase Chain Reaction Assay

2002 ◽  
Vol 65 (11) ◽  
pp. 1712-1716 ◽  
Author(s):  
PAWIN PADUNGTOD ◽  
ROBERT HANSON ◽  
DAVID L. WILSON ◽  
JULIA BELL ◽  
JOHN E. LINZ ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Campylobacter Jejuni ◽  
Rrna Genes ◽  
23S Rrna ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Gyra Gene ◽  
Test Kit ◽  
Polymerase Chain ◽  
Conventional Test

A rapid 5′ nuclease fluorogenic polymerase chain reaction (PCR) assay for identifying Campylobacter jejuni was applied to Campylobacter isolates from chicken cloacal and carcass swabs collected from three chicken farms and a slaughterhouse in Thailand. The primers and the probe were based on the sequence of the gyrA gene in C. jejuni. C. jejuni isolates were identified by fluorogenic PCR assay of bacterial cells directly from Campylobacter-selective agar medium. This assay allowed the identification of C. jejuni within 1 day after colonies appeared on selective media. The fluorogenic PCR assay yielded results comparable to those of the conventional test kit (kappa = 0.76) but required less time. When the two methods disagreed with regard to species identification, results were confirmed by PCR restriction fragment length polymorphism of 23S rRNA genes. In these instances, the fluorogenic PCR assay correctly identified more isolates of C. jejuni than did the conventional test kit (six of seven isolates were unidentifiable by the conventional test kit). The fluorogenic PCR assay is a rapid and specific method that outperforms the conventional test kit in the identification of C. jejuni from environmental samples.

scholarly journals A Polymerase Chain Reaction Protocol for the Detection of Xanthomonas albilineans, the Causal Agent of Sugarcane Leaf Scald Disease

Plant Disease ◽  
1997 ◽  
Vol 81 (2) ◽  
pp. 189-194 ◽  
Author(s):  
Y.-B. Pan ◽  
M. P. Grisham ◽  
D. M. Burner
Keyword(s):  
Polymerase Chain Reaction ◽  
Blot Hybridization ◽  
Causal Agent ◽  
Rrna Genes ◽  
23S Rrna ◽  
Dot Blot ◽  
Chain Reaction ◽  
Leaf Scald ◽  
Polymerase Chain ◽  
Xanthomonas Albilineans

A polymerase chain reaction (PCR) protocol was developed that amplified a 360-bp DNA product unique to Xanthomonas albilineans (Xa), the causal agent of sugarcane leaf scald disease. The assay utilizes previously described PCR primers that target the intergenic transcribed spacer (ITS) region between the 16S and 23S rRNA genes. Primer pair Ala4/L1 allowed amplification of a 360-bp DNA fragment from 71 Xa strains including representatives of serovars I, II, and III. Fragments of different sizes were also amplified from three unidentified saprophytic bacteria from sugarcane. Xa could be detected at a lower bacterial concentration with the PCR protocol than with a serological dot blot assay. With PCR, as little as 1.25 pg of Xa genomic DNA (125 fg if followed by Southern blot hybridization), or as few as 0 to 5 CFU of Xa per reaction were detected from infected sugarcane sap and leaf diffusate. Five CFU of Xa per reaction were detected from suspension culture. The PCR protocol provides a rapid, reliable, and economical tool for routine detection and identification of Xa.

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