Construction of the primary physical map of rice chromosome 12

2000 ◽  
Vol 5 (2) ◽  
pp. 238-244
Author(s):  
Fu Bin-Ying ◽  
Zhu Ying-Guo ◽  
Li Zhi-Kang
Keyword(s):  
Physical Map ◽  
Rice Chromosome ◽  
Chromosome 12

High-Resolution Pachytene Chromosome Mapping of Bacterial Artificial Chromosomes Anchored by Genetic Markers Reveals the Centromere Location and the Distribution of Genetic Recombination Along Chromosome 10 of Rice

Genetics ◽  
2001 ◽  
Vol 157 (4) ◽  
pp. 1749-1757 ◽  
Author(s):  
Zhukuan Cheng ◽  
Gernot G Presting ◽  
C Robin Buell ◽  
Rod A Wing ◽  
Jiming Jiang
Keyword(s):  
Large Scale ◽  
Genetic Recombination ◽  
Physical Map ◽  
Rice Chromosome ◽  
Resolving Power ◽  
Pachytene Chromosome ◽  
Chromosome 10 ◽  
Bac Clone ◽  
Fish Mapping ◽  
Artificial Chromosomes

AbstractLarge-scale physical mapping has been a major challenge for plant geneticists due to the lack of techniques that are widely affordable and can be applied to different species. Here we present a physical map of rice chromosome 10 developed by fluorescence in situ hybridization (FISH) mapping of bacterial artificial chromosome (BAC) clones on meiotic pachytene chromosomes. This physical map is fully integrated with a genetic linkage map of rice chromosome 10 because each BAC clone is anchored by a genetically mapped restriction fragment length polymorphism marker. The pachytene chromosome-based FISH mapping shows a superior resolving power compared to the somatic metaphase chromosome-based methods. The telomere-centromere orientation of DNA clones separated by 40 kb can be resolved on early pachytene chromosomes. Genetic recombination is generally evenly distributed along rice chromosome 10. However, the highly heterochromatic short arm shows a lower recombination frequency than the largely euchromatic long arm. Suppression of recombination was found in the centromeric region, but the affected region is far smaller than those reported in wheat and barley. Our FISH mapping effort also revealed the precise genetic position of the centromere on chromosome 10.

A physical map of the chromosome 12 centromere

2003 ◽  
Vol 103 (1-2) ◽  
pp. 63-73 ◽  
Author(s):  
J.R. Vermeesch ◽  
H. Duhamel ◽  
P. Raeymaekers ◽  
K. van Zand ◽  
P. Verhasselt ◽  
...  
Keyword(s):  
Physical Map ◽  
Chromosome 12

Mapping of a brown planthopper (Nilaparvata lugens Stål) resistance gene Bph9 on the long arm of rice chromosome 12

2001 ◽  
Vol 29 (3-4) ◽  
pp. 245-250 ◽  
Author(s):  
Kazumasa Murata ◽  
Manabu Fujiwara ◽  
Hiroshi Murai ◽  
Shigeo Takumi ◽  
Naoki Mori ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Brown Planthopper ◽  
Rice Chromosome ◽  
Nilaparvata Lugens ◽  
Chromosome 12 ◽  
Nilaparvata Lugens Stål

scholarly journals Physical and Genetic Mapping in the GrassesLolium perenneandFestuca pratensis

Genetics ◽  
2002 ◽  
Vol 161 (1) ◽  
pp. 315-324 ◽  
Author(s):  
J King ◽  
I P Armstead ◽  
I S Donnison ◽  
H M Thomas ◽  
R N Jones ◽  
...  
Keyword(s):  
Large Scale ◽  
Physical Map ◽  
Rice Chromosome ◽  
Nucleolar Organizer ◽  
Physical Distance ◽  
Chromosome 1 ◽  
Grass Species ◽  
Nucleolar Organizer Regions ◽  
Single Chromosome ◽  
Large Scale Analysis

AbstractA single chromosome of the grass species Festuca pratensis has been introgressed into Lolium perenne to produce a diploid monosomic substitution line (2n = 2x = 14). In this line recombination occurs throughout the length of the F. pratensis/L. perenne bivalent. The F. pratensis chromosome and recombinants between it and its L. perenne homeologue can be visualized using genomic in situ hybridization (GISH). GISH junctions represent the physical locations of sites of recombination, enabling a range of recombinant chromosomes to be used for physical mapping of the introgressed F. pratensis chromosome. The physical map, in conjunction with a genetic map composed of 104 F. pratensis-specific amplified fragment length polymorphisms (AFLPs), demonstrated: (1) the first large-scale analysis of the physical distribution of AFLPs; (2) variation in the relationship between genetic and physical distance from one part of the F. pratensis chromosome to another (e.g., variation was observed between and within chromosome arms); (3) that nucleolar organizer regions (NORs) and centromeres greatly reduce recombination; (4) that coding sequences are present close to the centromere and NORs in areas of low recombination in plant species with large genomes; and (5) apparent complete synteny between the F. pratensis chromosome and rice chromosome 1.

Genetic and physical mapping of blast resistance gene Pi-42(t) on the short arm of rice chromosome 12

2009 ◽  
Vol 25 (2) ◽  
pp. 217-228 ◽  
Author(s):  
P. Kumar ◽  
S. Pathania ◽  
P. Katoch ◽  
T. R. Sharma ◽  
P. Plaha ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Physical Mapping ◽  
Blast Resistance ◽  
Rice Chromosome ◽  
Chromosome 12 ◽  
Blast Resistance Gene ◽  
Genetic And Physical Mapping

scholarly journals Reconstruction of Linkage Map with Isozyme, Morphological and Physiological Markers in Rice Chromosome 12.

1992 ◽  
Vol 42 (2) ◽  
pp. 235-244 ◽  
Author(s):  
Ryuji ISHIKAWA ◽  
Takeo HARADA ◽  
Minoru NIIZEKI ◽  
Ken-ichi SAITO
Keyword(s):  
Linkage Map ◽  
Rice Chromosome ◽  
Chromosome 12 ◽  
Physiological Markers
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