Commercial kits for plant nucleic acid purification

2001 ◽  
Vol 19 (1) ◽  
pp. 2-2
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Purification ◽  
Commercial Kits

scholarly journals Filter paper-based spin column for low throughput nucleic acid purification

2018 ◽  
Author(s):  
Rui Shi ◽  
Ramsey S. Lewis ◽  
Dilip R. Panthee
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Filter Paper ◽  
Genomic Dna ◽  
Total Rna ◽  
Binding Material ◽  
Pcr Product ◽  
Spin Column ◽  
Nucleic Acid Purification ◽  
Commercial Kits

AbstractWe describe a method of recharging used spin column or assembling homemade spin column using filter paper as binding material for low throughput nucleic acid purification. We evaluated the efficiency of filter paper based spin columns in the purification of different type of nucleic acids. For instance, by following protocols of respective commercial kits, we found that filter paper to be a useful binding material for purification of many types of nucleic acids, including plant genomic DNA, plant total RNA, PCR product, and DNA from agarose gels. We also found that filter paper has a weak binding affinity to plasmid DNA in tested miniprep protocols. Also, we present the protocols of using filter paper recharged spin column or homemade spin column for low throughput purification of plant genomic DNA and plant total RNA with commercial kit buffer leftover and less expensive homemade buffer.

scholarly journals Simple Synthesis of Functionalized Paramagnetic Beads for Nucleic Acid Purification and Manipulation

BIO-PROTOCOL ◽  
2019 ◽  
Vol 9 (20) ◽  
Author(s):  
Phil Oberacker ◽  
Peter Stepper ◽  
Donna Bond ◽  
Katharina Hipp ◽  
Timothy Hore ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Simple Synthesis ◽  
Nucleic Acid Purification ◽  
Paramagnetic Beads

Recent developments of magnetic beads for use in nucleic acid purification

1998 ◽  
Vol 816 (1) ◽  
pp. 107-111 ◽  
Author(s):  
Peter R Levison ◽  
Stephen E Badger ◽  
Jon Dennis ◽  
Prit Hathi ◽  
Martin J Davies ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Magnetic Beads ◽  
Recent Developments ◽  
Nucleic Acid Purification

scholarly journals Immiscible Phase Nucleic Acid Purification Eliminates PCR Inhibitors with a Single Pass of Paramagnetic Particles through a Hydrophobic Liquid

2010 ◽  
Vol 12 (5) ◽  
pp. 620-628 ◽  
Author(s):  
Kunal Sur ◽  
Sally M. McFall ◽  
Emilie T. Yeh ◽  
Sujit R. Jangam ◽  
Mark A. Hayden ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Pcr Inhibitors ◽  
Single Pass ◽  
Nucleic Acid Purification ◽  
Immiscible Phase

One-step purification and concentration of DNA in porous membranes for point-of-care applications

Lab on a Chip ◽  
2015 ◽  
Vol 15 (12) ◽  
pp. 2647-2659 ◽  
Author(s):  
S. A. Byrnes ◽  
J. D. Bishop ◽  
L. Lafleur ◽  
J. R. Buser ◽  
B. Lutz ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Point Of Care ◽  
Porous Membranes ◽  
Complex Samples ◽  
One Step ◽  
Single User ◽  
Nucleic Acid Purification

Nucleic acid purification in porous membranes at the point-of-care from complex samples including nasal matrix and blood using a single-user step.

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