The use of the luciferase reporter system forin planta gene expression studies

2000 ◽  
Vol 18 (2) ◽  
pp. 143-144 ◽  
Author(s):  
Wessel Van Leeuwen ◽  
Marc J. M. Hagendoorn ◽  
Tom Ruttink ◽  
Remco Van Poecke ◽  
Linus H. W. Van Der Plas ◽  
...  
Keyword(s):  
Gene Expression ◽  
Luciferase Reporter ◽  
Reporter System ◽  
Expression Studies ◽  
Luciferase Reporter System ◽  
Gene Expression Studies

scholarly journals LncRNA FGD5-AS1 Facilitates the Radioresistance of Breast Cancer Cells by Enhancing MACC1 Expression Through Competitively Sponging miR-497-5p

2021 ◽  
Vol 11 ◽  
Author(s):  
Ji Li ◽  
Changjiang Lei ◽  
Bineng Chen ◽  
Qingfang Zhu
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Cell Lines ◽  
Radiation Resistance ◽  
Luciferase Reporter ◽  
Weekly Dose ◽  
Loss Of Function ◽  
Reporter System ◽  
X Ray ◽  
Luciferase Reporter System

BackgroundLncRNA-FGD5-AS1, as an oncogene, participates in the development and progress of various cancers. However, the exact role and the molecular mechanisms by which FGD5-AS1 regulates radiosensitivity in breast cancer (BC) remains largely unknown.MethodsWe used X-Ray weekly-dose-increase method to establish radiation-resistance cell lines. Bioinformatics tools analyze the expression of FGD5-AS1 in breast cancer tissue and evaluated the relationship between FGD5-AS1 and clinic-pathological features. CCK-8 and colony formation were used to analyze cell proliferation. Western blotting and qPCR were applied to detect protein and gene expression, respectively. RNA interference was used to knock down the endogenous gene expression. Luciferase reporter system and immunoprecipitates were applied to verify the target of FGD5-AS1.ResultFGD5-AS1 was overexpressed in BC tissues and radiation-resistance cell lines. Higher levels of FGD5-AS1 predicted poorer clinical characteristics and prognosis. Loss-of-function FGD5-AS1 sensitized BC cells to X-ray, meanwhile, the cell gained radiation-resistance when exogenous FGD5-AS1 was expressed. FGD5-AS1 depletion arrested cells at G0/G1 and triggers cell apoptosis. The starBase database (ENCORI), predicted binding site of miR-497-5p in FGD5-AS1 sequence, and luciferase reporter system and immunoprecipitates verified miR-497-5p was the target of FGD5-AS1. Furthermore, MACC1 was predicted and verified as the target of miR-497-5p. Loss-of-function FGD5-AS1 sensitized ionizing radiation was rescued by the up-regulation of MACC1 and the inhibition of miR-497.ConclusionFGD5-AS1 displays an oncogene profile in CRC; patients with high expression of FGD5-AS1 should benefit less from radiotherapy and need a more frequent follow-up. Besides, FGD5-AS1 may be a potential therapeutic target for CRC.

Evaluation and Comparison of the GUS, LUC and GFP Reporter System for Gene Expression Studies in Plants

Plant Biology ◽  
2003 ◽  
Vol 5 (2) ◽  
pp. 103-115 ◽  
Author(s):  
N. C. A. Ruijter ◽  
J. Verhees ◽  
W. Leeuwen ◽  
A. R. Krol
Keyword(s):  
Gene Expression ◽  
Reporter System ◽  
Expression Studies ◽  
Gfp Reporter ◽  
Gene Expression Studies

scholarly journals Phytochrome gene expression studies: PHYE is required for FR-induced expression of PHYA and PHYD suppresses expression of PHYA

2021 ◽  
Author(s):  
Umidjon Shapulatov ◽  
Mara Meisenburg ◽  
mark van hoogdalem ◽  
Alexander van Hall ◽  
Wim Van Ieperen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Plant Growth ◽  
Luciferase Reporter ◽  
Ambient Light ◽  
Light Responses ◽  
Expression Studies ◽  
Controlled Environments ◽  
R:Fr Ratio ◽  
Gene Expression Studies ◽  
Established Role

Arabidopsis has five phytochrome (PHY) genes for sensing the Red:Far Red (R:FR) ratio in ambient light, of which PHYA has an established role in responses to FR. To study whether and how PHYs may influence each other's transcription, PHY-Luciferase reporter plants (pPHYA:LUC, pPHYB:LUC, pPHYC:LUC, pPHYD:LUC and pPHYE:LUC) were constructed. Subsequently, reporter lines representative for each PHY were crossed into each of the five single phy-mutant backgrounds. Reporter activities in WT and phy mutant was studied under diurnal mixed (R, B, FR), R, FR or B LED light in seedling or rosette plants. Both pPHYA:LUC and pPHYB:LUC show strong induction under FR. Full FR upregulation of both PHYA and PHYB is dependent on PHYE, identifying PHYE as a novel sensor for FR light responses. Results also show that PHYA expression is strongly suppressed by PHYD. Results were confirmed for expression of endogenous PHYA and PHYB, albeit with different dynamics compared to the LUC reporters. Profiling of pPHYA:LUC and pPHYB:LUC reporters suggest gating of FR responses. Manipulation of PHY expression levels by FR may provide a novel basis for manipulating plant growth in controlled environments.

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