scholarly journals Somatic embryogenesis and plant regeneration from floral explants of cacao (Theobroma cacao L.) using thidiazuron

1998 ◽  
Vol 34 (4) ◽  
pp. 293-299 ◽  
Author(s):  
Zhijian Li ◽  
Abdoulaye Traore ◽  
Siela Maximova ◽  
Mark J. Guiltinan
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Theobroma Cacao ◽  
Theobroma Cacao L ◽  
Floral Explants

scholarly journals Somatic embryogenesis and plant regeneration in elite clones of Theobroma cacao

2008 ◽  
Vol 43 (10) ◽  
pp. 1433-1436 ◽  
Author(s):  
Thiago Édson Ribeiro da Silva ◽  
Luciana Cardoso Cidade ◽  
Fátima Cerqueira Alvim ◽  
Júlio Cézar de Mattos Cascardo ◽  
Marcio Gilberto Cardoso Costa
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Carbon Source ◽  
Embryogenic Callus ◽  
Theobroma Cacao ◽  
Somatic Embryos ◽  
Embryogenic Calli ◽  
Embryogenic Potential ◽  
Petri Dishes ◽  
Theobroma Cacao L

The objective of this work was to evaluated a procedure for somatic embryogenesis and regeneration of cacao (Theobroma cacao L.) elite clones. Petal explants from cacao clones TSH 565 and TSH 1188 were cultured on PCG and SCG-2 media, for calli growth. Somatic embryos were formed on the surface of embryogenic calli after transfer to embryo development (ED) medium. Clone TSH 565 showed a higher embryogenic potential than TSH 1188. The best combination of carbon source for embryo induction in ED medium was genotype-specific. Embryogenic callus formations increased in micropore tape-sealed Petri dishes, irrespective of cacao genotype. Mature somatic embryos were successfully converted into plantlets.

scholarly journals Packaging of Post Acclimatized Somatic Embryogenesis Cocoa Plantlet (Theobroma cacao L.)

2009 ◽  
Vol 25 (1) ◽  
Author(s):  
Soedarsianto Soedarsianto ◽  
Teguh Iman Santoso
Keyword(s):  
Somatic Embryogenesis ◽  
Distribution System ◽  
Theobroma Cacao ◽  
Storage Period ◽  
Storage Condition ◽  
Clonal Plants ◽  
Result Show ◽  
Fallen Leaves ◽  
Planting Materials ◽  
Theobroma Cacao L

Clonal plants that produced by somatic embryogenesis technique is one of the best choice to produce supperior clonal cacao (Theobroma cacao L.) planting materials. The somatic embryogenesis technique is a possible way for massive propagation, the outcome is true to type plants, the architecture similarity that the seedlings but there is not segregation like seedlings plants. At present mass production started of plantlets production until post-acclimatized plantlets of somatic embryogenesis cocoa was done at Indonesian Coffee and Cocoa Research Institute. Distribution system of the planting materials to whole areas in form of as up-rooted post-acclimatized plantlet. Some problems identified to reduce probability of decreasing viability of up-rooted post-acclimatized plantlets and one of them is extreme internal water deficit. This research investigate of the influece storage condition (air tight and non-air tight) and box storage (mica plastic and cardboardbox). The first experiment result show, there is no significant different between mica plastic and cardboard box usage for storage of post-acclimatized cocoa pantlet. Viability of up-rooted post acclimatized cocoa plantlet influenced exactly by air tight and non-air tight storage condition. Air tight storage condition have better viability of up-rooted post acclimatised (81,58%) than non-air tight storage condition (65,00%). Leaf sanasence on air tight storage condition (10,33%) lower than non-air tight storage (32,58%). There is not significantly on volume storage per plantlet between 4.416 cm3 and 12.600 cm3. Relationship between fallen leaves and cocoa planlet viability follow negative linear correlation y = -1,4719x + 104,88 (R2 = 0,9772). The second experiment treatment showed that maximal storage periode of post cclimatized cocoa plantlet just until 6 days stored (97%) and not significant with 3 days one. Viability of post acclimatized cocoa plantlet decreased after 6 days storage period.Key words : Somatic embryogenesis, post acclimatized cacao plantlet, storage condition, box storage, volume storage, storage period and viability.

Field performance of Theobroma cacao L. plants propagated via somatic embryogenesis

2008 ◽  
Vol 44 (6) ◽  
pp. 487-493 ◽  
Author(s):  
Siela N. Maximova ◽  
Ann Young ◽  
Sharon Pishak ◽  
Mark J. Guiltinan
Keyword(s):  
Somatic Embryogenesis ◽  
Theobroma Cacao ◽  
Field Performance ◽  
Theobroma Cacao L

scholarly journals Effect of antioxidants on the callus induction and the development of somatic embryogenesis of cocoa [Theobroma cacao (L.)]

2017 ◽  
Vol 11 (1) ◽  
pp. 25-31 ◽  
Author(s):  
Kouassi Kan Modeste ◽  
◽  
Manlé Tokpapon Eliane ◽  
Koné Daouda ◽  
Soumahoro André Brahima ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Callus Induction ◽  
Theobroma Cacao ◽  
Theobroma Cacao L

Epigenetics of long-term somatic embryogenesis in Theobroma cacao L.: DNA methylation and recovery of embryogenic potential

2017 ◽  
Vol 131 (2) ◽  
pp. 295-305 ◽  
Author(s):  
Liliana Alexandra Pila Quinga ◽  
Hugo Pacheco de Freitas Fraga ◽  
Leila do Nascimento Vieira ◽  
Miguel Pedro Guerra
Keyword(s):  
Dna Methylation ◽  
Somatic Embryogenesis ◽  
Theobroma Cacao ◽  
Embryogenic Potential ◽  
Theobroma Cacao L
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