Brush border enzyme activities in the small intestine after long-term gliadin feeding in animal models of human cœliac disease

1998 ◽  
Vol 43 (5) ◽  
pp. 497-500 ◽  
Author(s):  
H. Kozáková ◽  
R. Štěpánková ◽  
J. Kolínská ◽  
M. A. Farré ◽  
D. P. Funda ◽  
...  
Keyword(s):  
Small Intestine ◽  
Animal Models ◽  
Coeliac Disease ◽  
Brush Border ◽  
Enzyme Activities ◽  
Brush Border Enzyme

scholarly journals Age related increase of brush border enzyme activities along the small intestine.

Gut ◽  
1988 ◽  
Vol 29 (11) ◽  
pp. 1557-1563 ◽  
Author(s):  
F Raul ◽  
F Gosse ◽  
M Doffoel ◽  
P Darmenton ◽  
J Y Wessely
Keyword(s):  
Small Intestine ◽  
Brush Border ◽  
Enzyme Activities ◽  
Age Related ◽  
Brush Border Enzyme ◽  
Related Increase

scholarly journals Age related increase of brush border enzyme activities along the small intestine.

Gut ◽  
1989 ◽  
Vol 30 (6) ◽  
pp. 887-888 ◽  
Author(s):  
P R Holt ◽  
D P Kotler ◽  
K Y Yeh
Keyword(s):  
Small Intestine ◽  
Brush Border ◽  
Enzyme Activities ◽  
Age Related ◽  
Brush Border Enzyme ◽  
Related Increase

Impact of Probiotic and Zinc on Brush-border Enzyme and Histoenzymatic Profile in the Small Intestine of Pre and Post-weaned Piglets

2020 ◽  
Author(s):  
Arup Kalita ◽  
M. Talukdar ◽  
K. Sarma ◽  
P.C. Kalita ◽  
J.M. Gali ◽  
...  
Keyword(s):  
Small Intestine ◽  
Treatment Group ◽  
Brush Border ◽  
Statistical Tests ◽  
Age Groups ◽  
Treated Group ◽  
Histochemical Staining ◽  
Weaned Piglets ◽  
Standard Management ◽  
Brush Border Enzyme

Background: Probiotics and zinc are commonly used and beneficial in pig production. This work aimed to assess the effect of probiotic and zinc on brush-border enzyme activity and histoenzymatic study of the small intestine in pre and post-weaned piglets. Methods: Eighteen LWY piglets were divided equally into control and treatment groups. The piglets were maintained in standard management conditions and were weaned at 28 days of age. The treatment group of piglets fed a mixture of probiotics orally @ 1.25 x 109 CFU/day and zinc @ 2000 ppm/day from birth to 10 days of age. At three different age-groups viz. day 20 (pre-weaning), day 30 (weaning) and day 60 (post-weaning), the animals were sacrificed. For disaccharidase enzyme estimation, the mucosal brush border of the small intestine was scrapped off and the experiment was conducted. For histoenzymatic assay, the small intestine samples were preserved in liquid nitrogen at -196ºC immediately after sacrifice. They were sectioned at 10µm thickness maintained at -20ºC and stained for different histochemical staining. The statistical analysis of the data using the appropriate statistical tests was also conducted. Result: The activity of different brush-border enzymes such as maltase, sucrase and lactase was more in the treatment group of piglets. The activity of different histochemical enzymes such as alkaline phosphatase, acid phosphatase, adenosine tri-phosphatase and non-specific esterase was increased in the treated group of piglets.

scholarly journals Preterm birth makes the immature intestine sensitive to feeding-induced intestinal atrophy

2005 ◽  
Vol 289 (4) ◽  
pp. R1212-R1222 ◽  
Author(s):  
Charlotte Reinhard Bjornvad ◽  
Mette Schmidt ◽  
Yvette Miata Petersen ◽  
Søren Krogh Jensen ◽  
Hanne Offenberg ◽  
...  
Keyword(s):  
Preterm Birth ◽  
Brush Border ◽  
Enzyme Activities ◽  
Formula Feeding ◽  
Oral Feeding ◽  
Microbial Colonization ◽  
Milk Formula ◽  
Mrna Levels ◽  
Brush Border Enzyme ◽  
And Function

Preterm birth and formula feeding predispose to small intestinal dysfunction, which may lead to necrotizing enterocolitis (NEC). In piglets, we tested whether the physiological and environmental transitions occurring at birth affect the response of the immature intestine to enteral feeding. Pig fetuses (106 days gestation, term = 115 days) were prepared with esophageal feeding tubes and fed either sow's colostrum ( n = 8) or infant formula ( n = 7) in utero. After 24 h of oral feeding, the pig fetuses were delivered by cesarean section and their gastrointestinal morphology and function were compared with those of preterm newborn (NB) littermates that were not fed ( n = 8) or fed colostrum ( n = 7) or formula ( n = 13) for 24 h after birth. Before birth, both colostrum and formula feeding resulted in marked increases in intestinal mass, brush-border enzyme activities, and plasma glucagon-like peptide 2 concentrations, to levels similar to those in NB colostrum-fed piglets. In contrast, NB formula-fed piglets showed reduced intestinal growth, decreased brush-border enzyme activities, and intestinal lesions, reflecting NEC. NB formula-fed pigs also showed impaired enterocyte endocytotic function and decreased antioxidative capacity, whereas brush-border enzyme mRNA levels were unaltered, relative to NB colostrum-fed pigs. Our results indicate that the feeding-induced growth and enzyme maturation of the immature intestine are not birth dependent. However, with a suboptimal diet (milk formula), factors related to preterm birth (e.g., microbial colonization and metabolic and endocrine changes) make the immature intestine sensitive to atrophy and development of NEC.

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